Seed oil of Rosa roxburghii Tratt against non-alcoholic fatty liver disease in vivo and in vitro through PPARα/PGC-1α-mediated mitochondrial oxidative metabolism

Publication type:
Journal article
Metadata:
Autoren
  • Hai-Yan Ni
  • Liang Yu
  • Xue-Lian Zhao
  • Li-Tao Wang
  • Chun-Jian Zhao
  • Han Huang
  • Han-Lin Zhu
  • Thomas Efferth
  • Cheng-Bo Gu
  • Yu-Jie Fu
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000913999300001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.phymed.2021.153919
eISSN
1618-095X
Externe Identifier
  • Clarivate Analytics Document Solution ID: 7X1YE
  • PubMed Identifier: 35104757
ISSN
0944-7113
Zeitschrift
PHYTOMEDICINE
Schlüsselwörter
  • Hepatology
  • Mitochondrial dysfunction
  • NALFD
  • Phytotherapy
  • Oxidative stress
  • PPAR alpha/PGC-1 alpha signaling
  • PBS, phosphate buffer saline
  • PGC-1 alpha, peroxisome proliferator-activated receptor gamma coactivator 1 alpha
  • p38 MAPK, p38 mitogen-activated protein kinase
  • PPAR alpha, peroxisome proliferator activated receptor alpha
  • TFAM, Mitochondrial transcription factor A
  • TGF-beta, transforming growth factor-beta
  • TNF-alpha, Tumor Necrosis Factor alpha
  • TNF-alpha, Tumor necrosis factor alpha
  • UCP1, uncoupling protein 1
Artikelnummer
ARTN 153919
Datum der Veröffentlichung
2022
Status
Published
Titel
Seed oil of <i>Rosa roxburghii</i> Tratt against non-alcoholic fatty liver disease <i>in vivo</i> and <i>in vitro</i> through PPARα/PGC-1α-mediated mitochondrial oxidative metabolism
Sub types
  • Article
Ausgabe der Zeitschrift
98

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Hai-Yan Ni
  • Liang Yu
  • Xue-Lian Zhao
  • Li–Tao Wang
  • Chun–Jian Zhao
  • Han Huang
  • Han-Lin Zhu
  • Thomas Efferth
  • Cheng-Bo Gu
  • Yu-Jie Fu
DOI
10.1016/j.phymed.2021.153919
ISSN
0944-7113
Zeitschrift
Phytomedicine
Sprache
en
Artikelnummer
153919
Paginierung
153919 - 153919
Datum der Veröffentlichung
2022
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.phymed.2021.153919
Datum der Datenerfassung
2023
Titel
Seed oil of Rosa roxburghii Tratt against non-alcoholic fatty liver disease in vivo and in vitro through PPARα/PGC-1α-mediated mitochondrial oxidative metabolism
Ausgabe der Zeitschrift
98

Data source: Crossref

Abstract
<h4>Background</h4>Non-alcoholic fatty liver disease (NAFLD), characterized by hepatic steatosis and hepatocyte injury, is an obesity-induced metabolic dysregulation with few available therapeutic options. Enhancement of the mitochondrial function was considered as an effective treatment for NALFD. Unsaturated fatty acids (UFAs) have been shown to have beneficial effects on metabolic syndrome disease such as hyperlipidemia, coronary artery disease and cardiovascular diseases. The seed oil of Rosa roxburghii Tratt (ORRT) was of high quality in terms of its high amount of unsaturated fatty acids. However, the effects of ORRT on NALFD have not been reported so far.<h4>Purpose</h4>The study aimed to evaluate the protective effects and molecular mechanism of ORRT for the treatment of NAFLD in vivo and in vitro.<h4>Methods</h4>The beneficial effects, especially improving the mitochondrial function, and the potential mechanism of ORRT on NAFLD were studied both in vivo and in vitro. Lipid levels were determined by triglyceride (TG), total cholesterol (TC), and Oil Red O staining. Oxidative stress and inflammation were assessed by detecting antioxidant enzyme activity, MDA content, and ELISA assay. Blood TG, TC, HDL-c and LDL-c levels were measured in HFD mice. Western blot analyses were used to determine the levels of the protein involved in fatty acid oxidation, oxidative metabolism, and mitochondria biogenesis and function. The mitochondrial membrane potential level was measured by JC-1 staining to teste the effect of ORRT on mitochondrial function in vitro. GW6471 (inhibitor of PPARα) was used to confirm the relationship between PPARα and PGC-1α.<h4>Results</h4>ORRT significantly restrained NAFLD progression by attenuating lipid accumulation, oxidative stress and inflammatory response. Furthermore, ORRT upregulated thermogenesis-related gene expressions, such as uncoupling protein 1 (UCP1) and p38 mitogen-activated protein kinase (p38 MAPK). The results showed that the expression of key genes involved in fatty acid oxidation (e.g., CPT-1α, ACADL, PPARα) and in mitochondrial biogenesis and function (e.g., TFAM, NRF1, PGC-1α, and COX IV) was significantly increased. Together with the observed MMP improvement, these findings suggested that ORRT activated the mitochondrial oxidative pathway. Additionally, GW6471 inhibited the ORRT on promoting the expression of PGC-1α, CPT-1α, and ACADL. In conclusion, ORRT possessed the potential to prevent lipid accumulation via the PPARα/PGC-1α signaling pathway, which could be developed as a natural health-promoting oil against NAFLD.
Addresses
  • College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
Autoren
  • Hai-Yan Ni
  • Liang Yu
  • Xue-Lian Zhao
  • Li-Tao Wang
  • Chun-Jian Zhao
  • Han Huang
  • Han-Lin Zhu
  • Thomas Efferth
  • Cheng-Bo Gu
  • Yu-Jie Fu
DOI
10.1016/j.phymed.2021.153919
eISSN
1618-095X
Externe Identifier
  • PubMed Identifier: 35104757
Open access
false
ISSN
0944-7113
Zeitschrift
Phytomedicine : international journal of phytotherapy and phytopharmacology
Sprache
eng
Medium
Print-Electronic
Online publication date
2022
Paginierung
153919
Datum der Veröffentlichung
2022
Status
Published
Datum der Datenerfassung
2022
Titel
Seed oil of Rosa roxburghii Tratt against non-alcoholic fatty liver disease in vivo and in vitro through PPARα/PGC-1α-mediated mitochondrial oxidative metabolism.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
98

Data source: Europe PubMed Central

Abstract
BACKGROUND: Non-alcoholic fatty liver disease (NAFLD), characterized by hepatic steatosis and hepatocyte injury, is an obesity-induced metabolic dysregulation with few available therapeutic options. Enhancement of the mitochondrial function was considered as an effective treatment for NALFD. Unsaturated fatty acids (UFAs) have been shown to have beneficial effects on metabolic syndrome disease such as hyperlipidemia, coronary artery disease and cardiovascular diseases. The seed oil of Rosa roxburghii Tratt (ORRT) was of high quality in terms of its high amount of unsaturated fatty acids. However, the effects of ORRT on NALFD have not been reported so far. PURPOSE: The study aimed to evaluate the protective effects and molecular mechanism of ORRT for the treatment of NAFLD in vivo and in vitro. METHODS: The beneficial effects, especially improving the mitochondrial function, and the potential mechanism of ORRT on NAFLD were studied both in vivo and in vitro. Lipid levels were determined by triglyceride (TG), total cholesterol (TC), and Oil Red O staining. Oxidative stress and inflammation were assessed by detecting antioxidant enzyme activity, MDA content, and ELISA assay. Blood TG, TC, HDL-c and LDL-c levels were measured in HFD mice. Western blot analyses were used to determine the levels of the protein involved in fatty acid oxidation, oxidative metabolism, and mitochondria biogenesis and function. The mitochondrial membrane potential level was measured by JC-1 staining to teste the effect of ORRT on mitochondrial function in vitro. GW6471 (inhibitor of PPARα) was used to confirm the relationship between PPARα and PGC-1α. RESULTS: ORRT significantly restrained NAFLD progression by attenuating lipid accumulation, oxidative stress and inflammatory response. Furthermore, ORRT upregulated thermogenesis-related gene expressions, such as uncoupling protein 1 (UCP1) and p38 mitogen-activated protein kinase (p38 MAPK). The results showed that the expression of key genes involved in fatty acid oxidation (e.g., CPT-1α, ACADL, PPARα) and in mitochondrial biogenesis and function (e.g., TFAM, NRF1, PGC-1α, and COX IV) was significantly increased. Together with the observed MMP improvement, these findings suggested that ORRT activated the mitochondrial oxidative pathway. Additionally, GW6471 inhibited the ORRT on promoting the expression of PGC-1α, CPT-1α, and ACADL. In conclusion, ORRT possessed the potential to prevent lipid accumulation via the PPARα/PGC-1α signaling pathway, which could be developed as a natural health-promoting oil against NAFLD.
Date of acceptance
2021
Autoren
  • Hai-Yan Ni
  • Liang Yu
  • Xue-Lian Zhao
  • Li-Tao Wang
  • Chun-Jian Zhao
  • Han Huang
  • Han-Lin Zhu
  • Thomas Efferth
  • Cheng-Bo Gu
  • Yu-Jie Fu
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/35104757
DOI
10.1016/j.phymed.2021.153919
eISSN
1618-095X
Zeitschrift
Phytomedicine
Schlüsselwörter
  • Hepatology
  • Mitochondrial dysfunction
  • NALFD
  • Oxidative stress
  • PBS, phosphate buffer saline
  • PGC-1α, peroxisome proliferator-activated receptor gamma coactivator 1α
  • PPARα, peroxisome proliferator activated receptor α
  • PPARα/PGC-1α signaling
  • Phytotherapy
  • TFAM, Mitochondrial transcription factor A
  • TGF-β, transforming growth factor-beta
  • TNF-α, Tumor Necrosis Factor alpha
  • TNF-α, Tumor necrosis factor-α
  • UCP1, uncoupling protein 1
  • p38 MAPK, p38 mitogen-activated protein kinase
Sprache
eng
Country
Germany
Paginierung
153919
PII
S0944-7113(21)00459-1
Datum der Veröffentlichung
2022
Status
Published
Titel
Seed oil of Rosa roxburghii Tratt against non-alcoholic fatty liver disease in vivo and in vitro through PPARα/PGC-1α-mediated mitochondrial oxidative metabolism.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
98

Data source: PubMed

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