Cytotoxicity of Crude Extract and Isolated Constituents of the Dichrostachys cinerea Bark towards Multifactorial Drug-Resistant Cancer Cells
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Armelle T Mbaveng
- Francois Damen
- James D Simo Mpetga
- Maurice D Awouafack
- Pierre Tane
- Victor Kuete
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000476755300001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1155/2019/8450158
- eISSN
- 1741-4288
- Externe Identifier
- Clarivate Analytics Document Solution ID: IK7FU
- PubMed Identifier: 31360210
- ISSN
- 1741-427X
- Zeitschrift
- EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
- Artikelnummer
- ARTN 8450158
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Titel
- Cytotoxicity of Crude Extract and Isolated Constituents of the Dichrostachys cinerea Bark towards Multifactorial Drug-Resistant Cancer Cells
- Sub types
- Article
- Ausgabe der Zeitschrift
- 2019
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:p>The effectiveness of anticancer chemotherapy is greatly impeded by the resistance of malignant cells to cytotoxic drugs. In this study, the cytotoxicity of the crude extract (DCB) and compounds isolated from the bark of<jats:italic>Dichrostachys cinerea,</jats:italic>namely, betulinic acid (<jats:bold>1</jats:bold>), glyceryl-1-hexacosanoate (<jats:bold>2</jats:bold>), 7-hydroxy-2-(4-hydroxyphenyl)-4<jats:italic>H</jats:italic>-chromen-4-one (<jats:bold>3</jats:bold>), and 6-hydroxy-2-(4-hydroxyphenyl)-4<jats:italic>H</jats:italic>-chromen-4-one (<jats:bold>4</jats:bold>), was investigated. The study was extended to the assessment of the mode of induction of apoptosis by DCB and compound<jats:bold>1</jats:bold>. The resazurin reduction assay was used for cytotoxicity studies. Assessments of cell cycle distribution, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) were performed by flow cytometry. Constituents of DCB were isolated by column chromatography. Triterpenoid<jats:bold>1</jats:bold>and flavone<jats:bold>4</jats:bold>had cytotoxic effects towards the 9 tested cancer cell lines with IC<jats:sub>50</jats:sub>values below 50<jats:italic>μ</jats:italic>M. The recorded IC<jats:sub>50</jats:sub>values varied from 7.65<jats:italic>μ</jats:italic>M (towards multidrug-resistant CEM-ADR5000 leukemia cells) to 44.17<jats:italic>μ</jats:italic>M (against HepG2 hepatocarcinoma cells) for<jats:bold>1</jats:bold>, 18.90<jats:italic>μ</jats:italic>M (CCRF-CEM leukemia cells) to 88.86<jats:italic>μ</jats:italic>M (against HCT116p53<jats:sup>+/+</jats:sup>colon adenocarcinoma cells) for<jats:bold>4,</jats:bold>and 0.02<jats:italic>μ</jats:italic>M (against CCRF-CEM cells) to 122.96<jats:italic>μ</jats:italic>M (against CEM/ADR5000 cells) for doxorubicin. DCB induced apoptosis in CCRF-CEM cells mostly mediated by MMP alteration and enhanced ROS production; compound<jats:bold>1</jats:bold>induced apoptosis through caspases activation and MMP alteration and increased ROS production.<jats:italic>Dichrostachys cinerea</jats:italic>is an interesting cytotoxic plant and deserves more studies leading to new antineoplastic agents to fight cancer and mostly leukemia.</jats:p>
- Autoren
- Armelle T Mbaveng
- Francois Damen
- James D Simo Mpetga
- Maurice D Awouafack
- Pierre Tane
- Victor Kuete
- Thomas Efferth
- DOI
- 10.1155/2019/8450158
- eISSN
- 1741-4288
- ISSN
- 1741-427X
- Zeitschrift
- Evidence-Based Complementary and Alternative Medicine
- Sprache
- en
- Paginierung
- 1 - 11
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Herausgeber
- Hindawi Limited
- Herausgeber URL
- http://dx.doi.org/10.1155/2019/8450158
- Datum der Datenerfassung
- 2023
- Titel
- Cytotoxicity of Crude Extract and Isolated Constituents of the<i>Dichrostachys cinerea</i>Bark towards Multifactorial Drug-Resistant Cancer Cells
- Ausgabe der Zeitschrift
- 2019
Data source: Crossref
- Abstract
- The effectiveness of anticancer chemotherapy is greatly impeded by the resistance of malignant cells to cytotoxic drugs. In this study, the cytotoxicity of the crude extract (DCB) and compounds isolated from the bark of <i>Dichrostachys cinerea,</i> namely, betulinic acid (<b>1</b>), glyceryl-1-hexacosanoate (<b>2</b>), 7-hydroxy-2-(4-hydroxyphenyl)-4<i>H</i>-chromen-4-one (<b>3</b>), and 6-hydroxy-2-(4-hydroxyphenyl)-4<i>H</i>-chromen-4-one (<b>4</b>), was investigated. The study was extended to the assessment of the mode of induction of apoptosis by DCB and compound <b>1</b>. The resazurin reduction assay was used for cytotoxicity studies. Assessments of cell cycle distribution, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) were performed by flow cytometry. Constituents of DCB were isolated by column chromatography. Triterpenoid <b>1</b> and flavone <b>4</b> had cytotoxic effects towards the 9 tested cancer cell lines with IC<sub>50</sub> values below 50 <i>μ</i>M. The recorded IC<sub>50</sub> values varied from 7.65 <i>μ</i>M (towards multidrug-resistant CEM-ADR5000 leukemia cells) to 44.17 <i>μ</i>M (against HepG2 hepatocarcinoma cells) for <b>1</b>, 18.90 <i>μ</i>M (CCRF-CEM leukemia cells) to 88.86 <i>μ</i>M (against HCT116p53<sup>+/+</sup> colon adenocarcinoma cells) for <b>4,</b> and 0.02 <i>μ</i>M (against CCRF-CEM cells) to 122.96 <i>μ</i>M (against CEM/ADR5000 cells) for doxorubicin. DCB induced apoptosis in CCRF-CEM cells mostly mediated by MMP alteration and enhanced ROS production; compound <b>1</b> induced apoptosis through caspases activation and MMP alteration and increased ROS production. <i>Dichrostachys cinerea</i> is an interesting cytotoxic plant and deserves more studies leading to new antineoplastic agents to fight cancer and mostly leukemia.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany.
- Autoren
- Armelle T Mbaveng
- Francois Damen
- James D Simo Mpetga
- Maurice D Awouafack
- Pierre Tane
- Victor Kuete
- Thomas Efferth
- DOI
- 10.1155/2019/8450158
- eISSN
- 1741-4288
- Externe Identifier
- PubMed Identifier: 31360210
- PubMed Central ID: PMC6644236
- Funding acknowledgements
- Alexander von Humboldt: CMR 1163890 GF-E
- Open access
- true
- ISSN
- 1741-427X
- Zeitschrift
- Evidence-based complementary and alternative medicine : eCAM
- Sprache
- eng
- Medium
- Electronic-eCollection
- Online publication date
- 2019
- Open access status
- Open Access
- Paginierung
- 8450158
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2019
- Titel
- Cytotoxicity of Crude Extract and Isolated Constituents of the <i>Dichrostachys cinerea</i> Bark towards Multifactorial Drug-Resistant Cancer Cells.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 2019
Files
http://downloads.hindawi.com/journals/ecam/2019/8450158.pdf https://europepmc.org/articles/PMC6644236?pdf=render
Data source: Europe PubMed Central
- Abstract
- The effectiveness of anticancer chemotherapy is greatly impeded by the resistance of malignant cells to cytotoxic drugs. In this study, the cytotoxicity of the crude extract (DCB) and compounds isolated from the bark of Dichrostachys cinerea, namely, betulinic acid (1), glyceryl-1-hexacosanoate (2), 7-hydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one (3), and 6-hydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one (4), was investigated. The study was extended to the assessment of the mode of induction of apoptosis by DCB and compound 1. The resazurin reduction assay was used for cytotoxicity studies. Assessments of cell cycle distribution, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) were performed by flow cytometry. Constituents of DCB were isolated by column chromatography. Triterpenoid 1 and flavone 4 had cytotoxic effects towards the 9 tested cancer cell lines with IC50 values below 50 μM. The recorded IC50 values varied from 7.65 μM (towards multidrug-resistant CEM-ADR5000 leukemia cells) to 44.17 μM (against HepG2 hepatocarcinoma cells) for 1, 18.90 μM (CCRF-CEM leukemia cells) to 88.86 μM (against HCT116p53+/+ colon adenocarcinoma cells) for 4, and 0.02 μM (against CCRF-CEM cells) to 122.96 μM (against CEM/ADR5000 cells) for doxorubicin. DCB induced apoptosis in CCRF-CEM cells mostly mediated by MMP alteration and enhanced ROS production; compound 1 induced apoptosis through caspases activation and MMP alteration and increased ROS production. Dichrostachys cinerea is an interesting cytotoxic plant and deserves more studies leading to new antineoplastic agents to fight cancer and mostly leukemia.
- Date of acceptance
- 2019
- Autoren
- Armelle T Mbaveng
- Francois Damen
- James D Simo Mpetga
- Maurice D Awouafack
- Pierre Tane
- Victor Kuete
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/31360210
- DOI
- 10.1155/2019/8450158
- Externe Identifier
- PubMed Central ID: PMC6644236
- ISSN
- 1741-427X
- Zeitschrift
- Evid Based Complement Alternat Med
- Sprache
- eng
- Country
- United States
- Paginierung
- 8450158
- Datum der Veröffentlichung
- 2019
- Status
- Published online
- Titel
- Cytotoxicity of Crude Extract and Isolated Constituents of the Dichrostachys cinerea Bark towards Multifactorial Drug-Resistant Cancer Cells.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 2019
Data source: PubMed
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