The Chinese herbal formula Free and Easy Wanderer ameliorates oxidative stress through KEAP1-NRF2/HO-1 pathway

Publication type:
Journal article
Metadata:
Autoren
  • Chunlan Hong
  • Jingming Cao
  • Ching-Fen Wu
  • Onat Kadioglu
  • Anja Schueffler
  • Ulrich Kauhl
  • Sabine M Klauck
  • Till Opatz
  • Eckhard Thines
  • Norbert W Paul
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000410739000049&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1038/s41598-017-10443-6
Externe Identifier
  • Clarivate Analytics Document Solution ID: FG9DM
  • PubMed Identifier: 28912423
ISSN
2045-2322
Zeitschrift
SCIENTIFIC REPORTS
Artikelnummer
ARTN 11551
Datum der Veröffentlichung
2017
Status
Published
Titel
The Chinese herbal formula <i>Free and Easy Wanderer</i> ameliorates oxidative stress through KEAP1-NRF2/HO-1 pathway
Sub types
  • Article
Ausgabe der Zeitschrift
7

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:p>Posttraumatic stress disorder (PTSD) gains a lot of attention due to high prevalence and strong psychological upset, but the etiology remains undefined and effective treatment is quite limited. Growing studies demonstrated the involvement of oxidative stress in various psychiatry diseases, suggesting anti-oxidation therapy might be a strategy for PTSD treatment.<jats:italic>Free and Easy Wanderer</jats:italic>(FAEW) is a poly-herbal drug clinically used in China for hundreds of years in the treatment of psychiatric disorder. We hypothesized that FAEW exerts clinical effects through the activity against oxidative stress with fluoxetine as antidepressant control drug. Our results revealed that FAEW significantly reduced both endogenous and H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>-induced exogenous ROS levels in the human glioblastoma T98G and neuroblastoma SH-SY5Y cell lines. Transcriptome-wide microarray analysis indicated NRF2/HO-1 as the common target of FAEW and fluoxetine. Western blotting assay proved that the two drugs promoted NRF2 release from KEAP1 in the cytoplasm and translocation to the nuclei in a KEAP1-dependent manner, the expression of the protein HO-1 increased accordingly, suggesting the participation of KEAP1-NRF2/HO-1 pathway. The chemical constituents of FAEW (<jats:italic>i.e</jats:italic>. paeoniflorin, baicalin) bound to KEAP1<jats:italic>in silico</jats:italic>, which hence might be the effective substances of FAEW. In conclusion, FAEW counteracted H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>-induced oxidative stress through KEAP1-NRF2/HO-1 pathway.</jats:p>
Autoren
  • Chunlan Hong
  • Jingming Cao
  • Ching-Fen Wu
  • Onat Kadioglu
  • Anja Schüffler
  • Ulrich Kauhl
  • Sabine M Klauck
  • Till Opatz
  • Eckhard Thines
  • Norbert W Paul
  • Thomas Efferth
DOI
10.1038/s41598-017-10443-6
eISSN
2045-2322
Ausgabe der Veröffentlichung
1
Zeitschrift
Scientific Reports
Sprache
en
Artikelnummer
11551
Online publication date
2017
Status
Published online
Herausgeber
Springer Science and Business Media LLC
Herausgeber URL
http://dx.doi.org/10.1038/s41598-017-10443-6
Datum der Datenerfassung
2023
Titel
The Chinese herbal formula Free and Easy Wanderer ameliorates oxidative stress through KEAP1-NRF2/HO-1 pathway
Ausgabe der Zeitschrift
7

Data source: Crossref

Abstract
Posttraumatic stress disorder (PTSD) gains a lot of attention due to high prevalence and strong psychological upset, but the etiology remains undefined and effective treatment is quite limited. Growing studies demonstrated the involvement of oxidative stress in various psychiatry diseases, suggesting anti-oxidation therapy might be a strategy for PTSD treatment. Free and Easy Wanderer (FAEW) is a poly-herbal drug clinically used in China for hundreds of years in the treatment of psychiatric disorder. We hypothesized that FAEW exerts clinical effects through the activity against oxidative stress with fluoxetine as antidepressant control drug. Our results revealed that FAEW significantly reduced both endogenous and H<sub>2</sub>O<sub>2</sub>-induced exogenous ROS levels in the human glioblastoma T98G and neuroblastoma SH-SY5Y cell lines. Transcriptome-wide microarray analysis indicated NRF2/HO-1 as the common target of FAEW and fluoxetine. Western blotting assay proved that the two drugs promoted NRF2 release from KEAP1 in the cytoplasm and translocation to the nuclei in a KEAP1-dependent manner, the expression of the protein HO-1 increased accordingly, suggesting the participation of KEAP1-NRF2/HO-1 pathway. The chemical constituents of FAEW (i.e. paeoniflorin, baicalin) bound to KEAP1 in silico, which hence might be the effective substances of FAEW. In conclusion, FAEW counteracted H<sub>2</sub>O<sub>2</sub>-induced oxidative stress through KEAP1-NRF2/HO-1 pathway.
Addresses
  • Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, 55128, Mainz, Germany.
Autoren
  • Chunlan Hong
  • Jingming Cao
  • Ching-Fen Wu
  • Onat Kadioglu
  • Anja Schüffler
  • Ulrich Kauhl
  • Sabine M Klauck
  • Till Opatz
  • Eckhard Thines
  • Norbert W Paul
  • Thomas Efferth
  • Thomas Efferth
DOI
10.1038/s41598-017-10443-6
eISSN
2045-2322
Externe Identifier
  • PubMed Identifier: 28912423
  • PubMed Central ID: PMC5599498
Open access
true
ISSN
2045-2322
Ausgabe der Veröffentlichung
1
Zeitschrift
Scientific reports
Schlüsselwörter
  • Neurons
  • Cell Line
  • Humans
  • Plants, Medicinal
  • Plant Extracts
  • Antioxidants
  • Microarray Analysis
  • Gene Expression Profiling
  • Oxidative Stress
  • NF-E2-Related Factor 2
  • Heme Oxygenase-1
  • Kelch-Like ECH-Associated Protein 1
Sprache
eng
Medium
Electronic
Online publication date
2017
Open access status
Open Access
Paginierung
11551
Datum der Veröffentlichung
2017
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2017
Titel
The Chinese herbal formula Free and Easy Wanderer ameliorates oxidative stress through KEAP1-NRF2/HO-1 pathway.
Sub types
  • Research Support, Non-U.S. Gov't
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
7

Files

https://www.nature.com/articles/s41598-017-10443-6.pdf https://europepmc.org/articles/PMC5599498?pdf=render

Data source: Europe PubMed Central

Abstract
Posttraumatic stress disorder (PTSD) gains a lot of attention due to high prevalence and strong psychological upset, but the etiology remains undefined and effective treatment is quite limited. Growing studies demonstrated the involvement of oxidative stress in various psychiatry diseases, suggesting anti-oxidation therapy might be a strategy for PTSD treatment. Free and Easy Wanderer (FAEW) is a poly-herbal drug clinically used in China for hundreds of years in the treatment of psychiatric disorder. We hypothesized that FAEW exerts clinical effects through the activity against oxidative stress with fluoxetine as antidepressant control drug. Our results revealed that FAEW significantly reduced both endogenous and H2O2-induced exogenous ROS levels in the human glioblastoma T98G and neuroblastoma SH-SY5Y cell lines. Transcriptome-wide microarray analysis indicated NRF2/HO-1 as the common target of FAEW and fluoxetine. Western blotting assay proved that the two drugs promoted NRF2 release from KEAP1 in the cytoplasm and translocation to the nuclei in a KEAP1-dependent manner, the expression of the protein HO-1 increased accordingly, suggesting the participation of KEAP1-NRF2/HO-1 pathway. The chemical constituents of FAEW (i.e. paeoniflorin, baicalin) bound to KEAP1 in silico, which hence might be the effective substances of FAEW. In conclusion, FAEW counteracted H2O2-induced oxidative stress through KEAP1-NRF2/HO-1 pathway.
Date of acceptance
2017
Autoren
  • Chunlan Hong
  • Jingming Cao
  • Ching-Fen Wu
  • Onat Kadioglu
  • Anja Schüffler
  • Ulrich Kauhl
  • Sabine M Klauck
  • Till Opatz
  • Eckhard Thines
  • Norbert W Paul
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/28912423
DOI
10.1038/s41598-017-10443-6
eISSN
2045-2322
Externe Identifier
  • PubMed Central ID: PMC5599498
Ausgabe der Veröffentlichung
1
Zeitschrift
Sci Rep
Schlüsselwörter
  • Antioxidants
  • Cell Line
  • Gene Expression Profiling
  • Heme Oxygenase-1
  • Humans
  • Kelch-Like ECH-Associated Protein 1
  • Microarray Analysis
  • NF-E2-Related Factor 2
  • Neurons
  • Oxidative Stress
  • Plant Extracts
  • Plants, Medicinal
Sprache
eng
Country
England
Paginierung
11551
PII
10.1038/s41598-017-10443-6
Datum der Veröffentlichung
2017
Status
Published online
Datum, an dem der Datensatz öffentlich gemacht wurde
2019
Titel
The Chinese herbal formula Free and Easy Wanderer ameliorates oxidative stress through KEAP1-NRF2/HO-1 pathway.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
7

Data source: PubMed

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