Applicability of a single-use bioreactor compared to a glass bioreactor for the fermentation of filamentous fungi and evaluation of the reproducibility of growth in pellet form
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Winda Soerjawinata
- Konstantin Schlegel
- Natalie Fuchs
- Anja Schueffler
- Tanja Schirmeister
- Roland Ulber
- Percy Kampeis
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000621428200001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1002/elsc.202000069
- eISSN
- 1618-2863
- Externe Identifier
- Clarivate Analytics Document Solution ID: RW2EP
- PubMed Identifier: 33976605
- ISSN
- 1618-0240
- Ausgabe der Veröffentlichung
- 5
- Zeitschrift
- ENGINEERING IN LIFE SCIENCES
- Schlüsselwörter
- fermentation
- pellets
- Penicillium sp
- protease inhibitor
- single‐
- use bioreactor
- Paginierung
- 324 - 339
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Titel
- Applicability of a single-use bioreactor compared to a glass bioreactor for the fermentation of filamentous fungi and evaluation of the reproducibility of growth in pellet form
- Sub types
- Article
- Ausgabe der Zeitschrift
- 21
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>The implementation of single‐use technologies offers several major advantages, e.g. prevention of cross‐contamination, especially when spore‐forming microorganisms are present. This study investigated the application of a single‐use bioreactor in batch fermentation of filamentous fungus <jats:italic>Penicillium</jats:italic> sp. (IBWF 040‐09) from the Institute of Biotechnology and Drug Research (IBWF), which is capable of intracellular production of a protease inhibitor against parasitic proteases as a secondary metabolite. Several modifications to the SU bioreactor were suggested in this study to allow the fermentation in which the fungus forms pellets. Simultaneously, fermentations in conventional glass bioreactor were also conducted as reference. Although there are significant differences in the construction material and gassing system, the similarity of the two types of bioreactors in terms of fungal metabolic activity and the reproducibility of fermentations could be demonstrated using statistic methods. Under the selected cultivation conditions, growth rate, yield coefficient, substrate uptake rate, and formation of intracellular protease‐inhibiting substance in the single‐use bioreactor were similar to those in the glass bioreactor.</jats:p>
- Autoren
- Winda Soerjawinata
- Konstantin Schlegel
- Natalie Fuchs
- Anja Schüffler
- Tanja Schirmeister
- Roland Ulber
- Percy Kampeis
- DOI
- 10.1002/elsc.202000069
- eISSN
- 1618-2863
- ISSN
- 1618-0240
- Ausgabe der Veröffentlichung
- 5
- Zeitschrift
- Engineering in Life Sciences
- Sprache
- en
- Online publication date
- 2021
- Paginierung
- 324 - 339
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1002/elsc.202000069
- Datum der Datenerfassung
- 2023
- Titel
- Applicability of a single‐use bioreactor compared to a glass bioreactor for the fermentation of filamentous fungi and evaluation of the reproducibility of growth in pellet form
- Ausgabe der Zeitschrift
- 21
Data source: Crossref
- Abstract
- The implementation of single-use technologies offers several major advantages, e.g. prevention of cross-contamination, especially when spore-forming microorganisms are present. This study investigated the application of a single-use bioreactor in batch fermentation of filamentous fungus <i>Penicillium</i> sp. (IBWF 040-09) from the Institute of Biotechnology and Drug Research (IBWF), which is capable of intracellular production of a protease inhibitor against parasitic proteases as a secondary metabolite. Several modifications to the SU bioreactor were suggested in this study to allow the fermentation in which the fungus forms pellets. Simultaneously, fermentations in conventional glass bioreactor were also conducted as reference. Although there are significant differences in the construction material and gassing system, the similarity of the two types of bioreactors in terms of fungal metabolic activity and the reproducibility of fermentations could be demonstrated using statistic methods. Under the selected cultivation conditions, growth rate, yield coefficient, substrate uptake rate, and formation of intracellular protease-inhibiting substance in the single-use bioreactor were similar to those in the glass bioreactor.
- Addresses
- Institute for Biotechnical Process Design Trier University of Applied Sciences, Environmental Campus Birkenfeld Hoppstädten-Weiersbach Germany.
- Autoren
- Winda Soerjawinata
- Konstantin Schlegel
- Natalie Fuchs
- Anja Schüffler
- Tanja Schirmeister
- Roland Ulber
- Percy Kampeis
- DOI
- 10.1002/elsc.202000069
- eISSN
- 1618-2863
- Externe Identifier
- PubMed Identifier: 33976605
- PubMed Central ID: PMC8092982
- Open access
- true
- ISSN
- 1618-0240
- Ausgabe der Veröffentlichung
- 5
- Zeitschrift
- Engineering in life sciences
- Sprache
- eng
- Medium
- Electronic-eCollection
- Online publication date
- 2021
- Open access status
- Open Access
- Paginierung
- 324 - 339
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2021
- Titel
- Applicability of a single-use bioreactor compared to a glass bioreactor for the fermentation of filamentous fungi and evaluation of the reproducibility of growth in pellet form.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 21
Files
https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/elsc.202000069 https://europepmc.org/articles/PMC8092982?pdf=render
Data source: Europe PubMed Central
- Abstract
- The implementation of single-use technologies offers several major advantages, e.g. prevention of cross-contamination, especially when spore-forming microorganisms are present. This study investigated the application of a single-use bioreactor in batch fermentation of filamentous fungus Penicillium sp. (IBWF 040-09) from the Institute of Biotechnology and Drug Research (IBWF), which is capable of intracellular production of a protease inhibitor against parasitic proteases as a secondary metabolite. Several modifications to the SU bioreactor were suggested in this study to allow the fermentation in which the fungus forms pellets. Simultaneously, fermentations in conventional glass bioreactor were also conducted as reference. Although there are significant differences in the construction material and gassing system, the similarity of the two types of bioreactors in terms of fungal metabolic activity and the reproducibility of fermentations could be demonstrated using statistic methods. Under the selected cultivation conditions, growth rate, yield coefficient, substrate uptake rate, and formation of intracellular protease-inhibiting substance in the single-use bioreactor were similar to those in the glass bioreactor.
- Date of acceptance
- 2021
- Autoren
- Winda Soerjawinata
- Konstantin Schlegel
- Natalie Fuchs
- Anja Schüffler
- Tanja Schirmeister
- Roland Ulber
- Percy Kampeis
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/33976605
- DOI
- 10.1002/elsc.202000069
- Externe Identifier
- PubMed Central ID: PMC8092982
- ISSN
- 1618-0240
- Ausgabe der Veröffentlichung
- 5
- Zeitschrift
- Eng Life Sci
- Schlüsselwörter
- Penicillium sp
- fermentation
- pellets
- protease inhibitor
- single‐use bioreactor
- Sprache
- eng
- Country
- Germany
- Paginierung
- 324 - 339
- PII
- ELSC1373
- Datum der Veröffentlichung
- 2021
- Status
- Published online
- Titel
- Applicability of a single-use bioreactor compared to a glass bioreactor for the fermentation of filamentous fungi and evaluation of the reproducibility of growth in pellet form.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 21
Data source: PubMed
- Autoren
- Winda Soerjawinata
- Konstantin Schlegel
- Natalie Fuchs
- Anja Schüffler
- Tanja Schirmeister
- Roland Ulber
- Percy Kampeis
- Zeitschrift
- Engineering in life sciences
- Artikelnummer
- 5
- Paginierung
- 324 - 339
- Datum der Veröffentlichung
- 2021
- Datum der Datenerfassung
- 2021
- Titel
- Applicability of a single-use bioreactor compared to a glass bioreactor for the fermentation of filamentous fungi and evaluation of the reproducibility of growth in pellet form
- Sub types
- article
- Ausgabe der Zeitschrift
- 21
Data source: Manual
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- Property of