The intracellular antibody capture technology (IACT): Towards a consensus sequence for intracellular antibodies

Publication type:
Journal article
Metadata:
Autoren
  • M Visintin
  • G Settanni
  • A Maritan
  • S Graziosi
  • JD Marks
  • A Cattaneo
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000174613300004&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1006/jmbi.2002.5392
eISSN
1089-8638
Externe Identifier
  • Clarivate Analytics Document Solution ID: 534WT
  • PubMed Identifier: 11916379
ISSN
0022-2836
Ausgabe der Veröffentlichung
1
Zeitschrift
JOURNAL OF MOLECULAR BIOLOGY
Schlüsselwörter
  • intrabodies
  • single-chain Fv
  • functional genomics
  • TAU
  • Alzheimer's disease
Paginierung
73 - 83
Datum der Veröffentlichung
2002
Status
Published
Titel
The intracellular antibody capture technology (IACT): Towards a consensus sequence for intracellular antibodies
Sub types
  • Article
Ausgabe der Zeitschrift
317

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Michela Visintin
  • Giovanni Settanni
  • Amos Maritan
  • Sergio Graziosi
  • James D Marks
  • Antonino Cattaneo
DOI
10.1006/jmbi.2002.5392
ISSN
0022-2836
Ausgabe der Veröffentlichung
1
Zeitschrift
Journal of Molecular Biology
Sprache
en
Paginierung
73 - 83
Datum der Veröffentlichung
2002
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1006/jmbi.2002.5392
Datum der Datenerfassung
2019
Titel
The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies
Ausgabe der Zeitschrift
317

Data source: Crossref

Abstract
We describe the application of an intracellular antibody capture technology (IACT) as a generic in vivo selection procedure for isolating intracellular antibodies or ICAbs. IACT was applied to the de novo selection of functional ICAbs against the microtubule-associated protein TAU, found in neurofibrillary lesions of Alzheimer's disease brains. A panel of 17 different ICAbs was created which bind TAU inside cells and the epitopes recognized by the selected ICAbs have been determined by an in vivo epitope mapping procedure. Finally, sequence analysis showed that the IACT-derived ICAbs are characterized by a common signature of conserved amino acid residues, suggesting that the IACT naturally selects a sort of "captured consensus sequence" for intracellular antibodies. The development of IACT, together with the possibility of scaling up in a high throughput and automated format, makes IACT a new enabling tool for target validation in functional genomics and global proteomics.
Addresses
  • International School for Advanced Studies (SISSA) and INFM Unit, 34013 Trieste, Italy.
Autoren
  • Michela Visintin
  • Giovanni Settanni
  • Amos Maritan
  • Sergio Graziosi
  • James D Marks
  • Antonino Cattaneo
DOI
10.1006/jmbi.2002.5392
eISSN
1089-8638
Externe Identifier
  • PubMed Identifier: 11916379
Open access
false
ISSN
0022-2836
Ausgabe der Veröffentlichung
1
Zeitschrift
Journal of molecular biology
Schlüsselwörter
  • COS Cells
  • CHO Cells
  • Intracellular Fluid
  • Fibroblasts
  • Animals
  • Humans
  • Alzheimer Disease
  • Peptide Library
  • tau Proteins
  • Antibodies
  • Microscopy, Fluorescence
  • Fluorescent Antibody Technique
  • Enzyme-Linked Immunosorbent Assay
  • Chromatography, Gel
  • Reproducibility of Results
  • Cloning, Molecular
  • Sequence Alignment
  • Epitope Mapping
  • Antibody Specificity
  • Amino Acid Sequence
  • Consensus Sequence
  • Mutation
  • Automation
  • Molecular Sequence Data
  • Cricetinae
Sprache
eng
Medium
Print
Paginierung
73 - 83
Datum der Veröffentlichung
2002
Status
Published
Datum der Datenerfassung
2002
Titel
The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
317

Data source: Europe PubMed Central

Abstract
We describe the application of an intracellular antibody capture technology (IACT) as a generic in vivo selection procedure for isolating intracellular antibodies or ICAbs. IACT was applied to the de novo selection of functional ICAbs against the microtubule-associated protein TAU, found in neurofibrillary lesions of Alzheimer's disease brains. A panel of 17 different ICAbs was created which bind TAU inside cells and the epitopes recognized by the selected ICAbs have been determined by an in vivo epitope mapping procedure. Finally, sequence analysis showed that the IACT-derived ICAbs are characterized by a common signature of conserved amino acid residues, suggesting that the IACT naturally selects a sort of "captured consensus sequence" for intracellular antibodies. The development of IACT, together with the possibility of scaling up in a high throughput and automated format, makes IACT a new enabling tool for target validation in functional genomics and global proteomics.
Autoren
  • Michela Visintin
  • Giovanni Settanni
  • Amos Maritan
  • Sergio Graziosi
  • James D Marks
  • Antonino Cattaneo
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/11916379
DOI
10.1006/jmbi.2002.5392
ISSN
0022-2836
Ausgabe der Veröffentlichung
1
Zeitschrift
J Mol Biol
Schlüsselwörter
  • Alzheimer Disease
  • Amino Acid Sequence
  • Animals
  • Antibodies
  • Antibody Specificity
  • Automation
  • CHO Cells
  • COS Cells
  • Chromatography, Gel
  • Cloning, Molecular
  • Consensus Sequence
  • Cricetinae
  • Enzyme-Linked Immunosorbent Assay
  • Epitope Mapping
  • Fibroblasts
  • Fluorescent Antibody Technique
  • Humans
  • Intracellular Fluid
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Mutation
  • Peptide Library
  • Reproducibility of Results
  • Sequence Alignment
  • tau Proteins
Sprache
eng
Country
Netherlands
Paginierung
73 - 83
PII
S0022283602953921
Datum der Veröffentlichung
2002
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2002
Titel
The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
317

Data source: PubMed

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