Shikonin Reduces Growth of Docetaxel-Resistant Prostate Cancer Cells Mainly through Necroptosis
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Sascha D Markowitsch
- Kira M Juetter
- Patricia Schupp
- Kristine Hauschulte
- Olesya Vakhrusheva
- Kimberly Sue Slade
- Anita Thomas
- Igor Tsaur
- Jindrich Jr Cinatl
- Martin Michaelis
- Thomas Efferth
- Axel Haferkamp
- Eva Juengel
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000623370900001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3390/cancers13040882
- eISSN
- 2072-6694
- Externe Identifier
- Clarivate Analytics Document Solution ID: QO8EK
- PubMed Identifier: 33672520
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- CANCERS
- Schlüsselwörter
- prostate cancer (PCa)
- docetaxel (DX) resistance
- shikonin (SHI)
- Traditional Chinese Medicine (TCM)
- growth inhibition
- apoptosis
- necroptosis
- Artikelnummer
- ARTN 882
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Titel
- Shikonin Reduces Growth of Docetaxel-Resistant Prostate Cancer Cells Mainly through Necroptosis
- Sub types
- Article
- Ausgabe der Zeitschrift
- 13
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:p>The prognosis for advanced prostate carcinoma (PCa) remains poor due to development of therapy resistance, and new treatment options are needed. Shikonin (SHI) from Traditional Chinese Medicine has induced antitumor effects in diverse tumor entities, but data related to PCa are scarce. Therefore, the parental (=sensitive) and docetaxel (DX)-resistant PCa cell lines, PC3, DU145, LNCaP, and 22Rv1 were exposed to SHI [0.1–1.5 μM], and tumor cell growth, proliferation, cell cycling, cell death (apoptosis, necrosis, and necroptosis), and metabolic activity were evaluated. Correspondingly, the expression of regulating proteins was assessed. Exposure to SHI time- and dose-dependently inhibited tumor cell growth and proliferation in parental and DX-resistant PCa cells, accompanied by cell cycle arrest in the G2/M or S phase and modulation of cell cycle regulating proteins. SHI induced apoptosis and more dominantly necroptosis in both parental and DX-resistant PCa cells. This was shown by enhanced pRIP1 and pRIP3 expression and returned growth if applying the necroptosis inhibitor necrostatin-1. No SHI-induced alteration in metabolic activity of the PCa cells was detected. The significant antitumor effects induced by SHI to parental and DX-resistant PCa cells make the addition of SHI to standard therapy a promising treatment strategy for patients with advanced PCa.</jats:p>
- Autoren
- Sascha D Markowitsch
- Kira M Juetter
- Patricia Schupp
- Kristine Hauschulte
- Olesya Vakhrusheva
- Kimberly Sue Slade
- Anita Thomas
- Igor Tsaur
- Jindrich Cinatl
- Martin Michaelis
- Thomas Efferth
- Axel Haferkamp
- Eva Juengel
- DOI
- 10.3390/cancers13040882
- eISSN
- 2072-6694
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Cancers
- Sprache
- en
- Online publication date
- 2021
- Paginierung
- 882 - 882
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/cancers13040882
- Datum der Datenerfassung
- 2021
- Titel
- Shikonin Reduces Growth of Docetaxel-Resistant Prostate Cancer Cells Mainly through Necroptosis
- Ausgabe der Zeitschrift
- 13
Data source: Crossref
- Abstract
- The prognosis for advanced prostate carcinoma (PCa) remains poor due to development of therapy resistance, and new treatment options are needed. Shikonin (SHI) from Traditional Chinese Medicine has induced antitumor effects in diverse tumor entities, but data related to PCa are scarce. Therefore, the parental (=sensitive) and docetaxel (DX)-resistant PCa cell lines, PC3, DU145, LNCaP, and 22Rv1 were exposed to SHI [0.1-1.5 μM], and tumor cell growth, proliferation, cell cycling, cell death (apoptosis, necrosis, and necroptosis), and metabolic activity were evaluated. Correspondingly, the expression of regulating proteins was assessed. Exposure to SHI time- and dose-dependently inhibited tumor cell growth and proliferation in parental and DX-resistant PCa cells, accompanied by cell cycle arrest in the G2/M or S phase and modulation of cell cycle regulating proteins. SHI induced apoptosis and more dominantly necroptosis in both parental and DX-resistant PCa cells. This was shown by enhanced pRIP1 and pRIP3 expression and returned growth if applying the necroptosis inhibitor necrostatin-1. No SHI-induced alteration in metabolic activity of the PCa cells was detected. The significant antitumor effects induced by SHI to parental and DX-resistant PCa cells make the addition of SHI to standard therapy a promising treatment strategy for patients with advanced PCa.
- Addresses
- Department of Urology and Pediatric Urology, University Medical Center Mainz, Langenbeckstraße 1, 55131 Mainz, Germany.
- Autoren
- Sascha D Markowitsch
- Kira M Juetter
- Patricia Schupp
- Kristine Hauschulte
- Olesya Vakhrusheva
- Kimberly Sue Slade
- Anita Thomas
- Igor Tsaur
- Jindrich Cinatl
- Martin Michaelis
- Thomas Efferth
- Axel Haferkamp
- Eva Juengel
- DOI
- 10.3390/cancers13040882
- eISSN
- 2072-6694
- Externe Identifier
- PubMed Identifier: 33672520
- PubMed Central ID: PMC7923752
- Funding acknowledgements
- Friedrich-Spicker-Stiftung: 01-2017
- Frankfurter Stiftung für krebskranke Kinder: 2020
- Friedrich-Spicker-Stiftung: 2020
- Hilfe für krebskranke Kinder Frankfurt e.V.: 2020
- Friedrich-Spicker-Stiftung: 2017
- Kent Cancer Trust: 2017
- Open access
- true
- ISSN
- 2072-6694
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Cancers
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2021
- Open access status
- Open Access
- Paginierung
- 882
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2021
- Titel
- Shikonin Reduces Growth of Docetaxel-Resistant Prostate Cancer Cells Mainly through Necroptosis.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 13
Files
https://www.mdpi.com/2072-6694/13/4/882/pdf?version=1614152051 https://europepmc.org/articles/PMC7923752?pdf=render
Data source: Europe PubMed Central
- Abstract
- The prognosis for advanced prostate carcinoma (PCa) remains poor due to development of therapy resistance, and new treatment options are needed. Shikonin (SHI) from Traditional Chinese Medicine has induced antitumor effects in diverse tumor entities, but data related to PCa are scarce. Therefore, the parental (=sensitive) and docetaxel (DX)-resistant PCa cell lines, PC3, DU145, LNCaP, and 22Rv1 were exposed to SHI [0.1-1.5 μM], and tumor cell growth, proliferation, cell cycling, cell death (apoptosis, necrosis, and necroptosis), and metabolic activity were evaluated. Correspondingly, the expression of regulating proteins was assessed. Exposure to SHI time- and dose-dependently inhibited tumor cell growth and proliferation in parental and DX-resistant PCa cells, accompanied by cell cycle arrest in the G2/M or S phase and modulation of cell cycle regulating proteins. SHI induced apoptosis and more dominantly necroptosis in both parental and DX-resistant PCa cells. This was shown by enhanced pRIP1 and pRIP3 expression and returned growth if applying the necroptosis inhibitor necrostatin-1. No SHI-induced alteration in metabolic activity of the PCa cells was detected. The significant antitumor effects induced by SHI to parental and DX-resistant PCa cells make the addition of SHI to standard therapy a promising treatment strategy for patients with advanced PCa.
- Date of acceptance
- 2021
- Autoren
- Sascha D Markowitsch
- Kira M Juetter
- Patricia Schupp
- Kristine Hauschulte
- Olesya Vakhrusheva
- Kimberly Sue Slade
- Anita Thomas
- Igor Tsaur
- Jindrich Cinatl
- Martin Michaelis
- Thomas Efferth
- Axel Haferkamp
- Eva Juengel
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/33672520
- DOI
- 10.3390/cancers13040882
- Externe Identifier
- PubMed Central ID: PMC7923752
- Funding acknowledgements
- Friedrich-Spicker-Stiftung: 01-2017
- Hilfe für krebskranke Kinder Frankfurt e.V.: 2020
- Frankfurter Stiftung für krebskranke Kinder: 2020
- Kent Cancer Trust: 2017
- ISSN
- 2072-6694
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Cancers (Basel)
- Schlüsselwörter
- Traditional Chinese Medicine (TCM)
- apoptosis
- docetaxel (DX) resistance
- growth inhibition
- necroptosis
- prostate cancer (PCa)
- shikonin (SHI)
- Sprache
- eng
- Country
- Switzerland
- PII
- cancers13040882
- Datum der Veröffentlichung
- 2021
- Status
- Published online
- Titel
- Shikonin Reduces Growth of Docetaxel-Resistant Prostate Cancer Cells Mainly through Necroptosis.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 13
Data source: PubMed
- Author's licence
- CC-BY
- Autoren
- Sascha D Markowitsch
- Kira M Juetter
- Patricia Schupp
- Kristine Hauschulte
- Olesya Vakhrusheva
- Kimberly Sue Slade
- Anita Thomas
- Igor Tsaur
- Jindrich Cinatl
- Martin Michaelis
- Thomas Efferth
- Axel Haferkamp
- Eva Juengel
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- JGU-Publikationen
- Resource version
- Published version
- DOI
- 10.3390/cancers13040882
- Funding acknowledgements
- Open Access-Publizieren Universität Mainz / Universitätsmedizin Mainz
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 2072-6694
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Cancers
- Schlüsselwörter
- 610 Medizin
- 610 Medical sciences
- Sprache
- eng
- Open access status
- Open Access
- Paginierung
- 882
- Datum der Veröffentlichung
- 2021
- Public URL
- https://openscience.ub.uni-mainz.de/handle/20.500.12030/5937
- Herausgeber
- MDPI
- Herausgeber URL
- https://doi.org/10.3390/cancers13040882
- Datum der Datenerfassung
- 2021
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Zugang
- Public
- Titel
- Shikonin reduces growth of docetaxel-resistant prostate cancer cells mainly through necroptosis
- Ausgabe der Zeitschrift
- 13
Files
markowitsch_sascha_d.-shikonin_reduc-20210514144150228.pdf
Data source: OPENSCIENCE.UB
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