Chemometric and Transcriptomic Profiling, Microtubule Disruption and Cell Death Induction by Secalonic Acid in Tumor Cells

Publication type:
Journal article
Metadata:
Autoren
  • Nadire Ozenver
  • Mona Dawood
  • Edmond Fleischer
  • Anette Klinger
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000556521700001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.3390/molecules25143224
eISSN
1420-3049
Externe Identifier
  • Clarivate Analytics Document Solution ID: MV7GO
  • PubMed Identifier: 32679716
Ausgabe der Veröffentlichung
14
Zeitschrift
MOLECULES
Schlüsselwörter
  • cancer
  • drug resistance
  • microarray analysis
  • multiple myeloma
  • mycotoxins
Artikelnummer
ARTN 3224
Datum der Veröffentlichung
2020
Status
Published
Titel
Chemometric and Transcriptomic Profiling, Microtubule Disruption and Cell Death Induction by Secalonic Acid in Tumor Cells
Sub types
  • Article
Ausgabe der Zeitschrift
25

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:p>Nature is an indispensable source of new drugs, providing unique bioactive lead structures for drug discovery. In the present study, secalonic acid F (SAF), a naturally occurring ergochrome pigment, was studied for its cytotoxicity against various leukemia and multiple myeloma cells by the resazurin assay. SAF exhibited cytotoxic activity on both leukemia and multiple myeloma cells. Generally, multiple myeloma cells were more sensitive to SAF than leukemia cells. NCI-H929 cells were the most affected cells among the tested panel of multiple myeloma cell lines and were taken for further studies to assess the mode of action of SAF on those cells. Cell cycle analysis revealed that SAF induced S and G2/M arrest in NCI-H929 cells. SAF-associated apoptosis and necrosis resulted in cytotoxicity. SAF further inclined the disassembly of the tubulin network, which may also account for its cytotoxicity. COMPARE and hierarchical cluster analyses of transcriptome-wide expression profiles of the NCI tumor cell line panel identified genes involved in numerous cellular processes (e.g., cell differentiation, cell migration, and other numerous signaling pathways) notably correlated with log10IC50 values for secalonic acid. In conclusion, the present study supports the therapeutic potential of SAF to treat multiple myeloma.</jats:p>
Autoren
  • Nadire Özenver
  • Mona Dawood
  • Edmond Fleischer
  • Anette Klinger
  • Thomas Efferth
DOI
10.3390/molecules25143224
eISSN
1420-3049
Ausgabe der Veröffentlichung
14
Zeitschrift
Molecules
Sprache
en
Online publication date
2020
Paginierung
3224 - 3224
Status
Published online
Herausgeber
MDPI AG
Herausgeber URL
http://dx.doi.org/10.3390/molecules25143224
Datum der Datenerfassung
2020
Titel
Chemometric and Transcriptomic Profiling, Microtubule Disruption and Cell Death Induction by Secalonic Acid in Tumor Cells
Ausgabe der Zeitschrift
25

Data source: Crossref

Abstract
Nature is an indispensable source of new drugs, providing unique bioactive lead structures for drug discovery. In the present study, secalonic acid F (SAF), a naturally occurring ergochrome pigment, was studied for its cytotoxicity against various leukemia and multiple myeloma cells by the resazurin assay. SAF exhibited cytotoxic activity on both leukemia and multiple myeloma cells. Generally, multiple myeloma cells were more sensitive to SAF than leukemia cells. NCI-H929 cells were the most affected cells among the tested panel of multiple myeloma cell lines and were taken for further studies to assess the mode of action of SAF on those cells. Cell cycle analysis revealed that SAF induced S and G2/M arrest in NCI-H929 cells. SAF-associated apoptosis and necrosis resulted in cytotoxicity. SAF further inclined the disassembly of the tubulin network, which may also account for its cytotoxicity. COMPARE and hierarchical cluster analyses of transcriptome-wide expression profiles of the NCI tumor cell line panel identified genes involved in numerous cellular processes (e.g., cell differentiation, cell migration, and other numerous signaling pathways) notably correlated with log<sub>10</sub>IC<sub>50</sub> values for secalonic acid. In conclusion, the present study supports the therapeutic potential of SAF to treat multiple myeloma.
Addresses
  • Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, 06100 Ankara, Turkey.
Autoren
  • Nadire Özenver
  • Mona Dawood
  • Edmond Fleischer
  • Anette Klinger
  • Thomas Efferth
DOI
10.3390/molecules25143224
eISSN
1420-3049
Externe Identifier
  • PubMed Identifier: 32679716
  • PubMed Central ID: PMC7397039
Funding acknowledgements
  • Deutscher Akademischer Austauschdienst: 57440917
Open access
true
ISSN
1420-3049
Ausgabe der Veröffentlichung
14
Zeitschrift
Molecules (Basel, Switzerland)
Schlüsselwörter
  • Cell Line, Tumor
  • Microtubules
  • Humans
  • Leukemia
  • Multiple Myeloma
  • Xanthones
  • Antineoplastic Agents
  • Gene Expression Profiling
  • Cell Cycle
  • Cell Death
  • Apoptosis
  • Molecular Structure
  • Metabolomics
  • Transcriptome
Sprache
eng
Medium
Electronic
Online publication date
2020
Open access status
Open Access
Paginierung
E3224
Datum der Veröffentlichung
2020
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2020
Titel
Chemometric and Transcriptomic Profiling, Microtubule Disruption and Cell Death Induction by Secalonic Acid in Tumor Cells.
Sub types
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
25

Files

https://www.mdpi.com/1420-3049/25/14/3224/pdf?version=1594804813 https://europepmc.org/articles/PMC7397039?pdf=render

Data source: Europe PubMed Central

Abstract
Nature is an indispensable source of new drugs, providing unique bioactive lead structures for drug discovery. In the present study, secalonic acid F (SAF), a naturally occurring ergochrome pigment, was studied for its cytotoxicity against various leukemia and multiple myeloma cells by the resazurin assay. SAF exhibited cytotoxic activity on both leukemia and multiple myeloma cells. Generally, multiple myeloma cells were more sensitive to SAF than leukemia cells. NCI-H929 cells were the most affected cells among the tested panel of multiple myeloma cell lines and were taken for further studies to assess the mode of action of SAF on those cells. Cell cycle analysis revealed that SAF induced S and G2/M arrest in NCI-H929 cells. SAF-associated apoptosis and necrosis resulted in cytotoxicity. SAF further inclined the disassembly of the tubulin network, which may also account for its cytotoxicity. COMPARE and hierarchical cluster analyses of transcriptome-wide expression profiles of the NCI tumor cell line panel identified genes involved in numerous cellular processes (e.g., cell differentiation, cell migration, and other numerous signaling pathways) notably correlated with log10IC50 values for secalonic acid. In conclusion, the present study supports the therapeutic potential of SAF to treat multiple myeloma.
Date of acceptance
2020
Autoren
  • Nadire Özenver
  • Mona Dawood
  • Edmond Fleischer
  • Anette Klinger
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/32679716
DOI
10.3390/molecules25143224
eISSN
1420-3049
Externe Identifier
  • PubMed Central ID: PMC7397039
Funding acknowledgements
  • Deutscher Akademischer Austauschdienst: 57440917
Ausgabe der Veröffentlichung
14
Zeitschrift
Molecules
Schlüsselwörter
  • cancer
  • drug resistance
  • microarray analysis
  • multiple myeloma
  • mycotoxins
  • Antineoplastic Agents
  • Apoptosis
  • Cell Cycle
  • Cell Death
  • Cell Line, Tumor
  • Gene Expression Profiling
  • Humans
  • Leukemia
  • Metabolomics
  • Microtubules
  • Molecular Structure
  • Multiple Myeloma
  • Transcriptome
  • Xanthones
Sprache
eng
Country
Switzerland
PII
molecules25143224
Datum der Veröffentlichung
2020
Status
Published online
Datum, an dem der Datensatz öffentlich gemacht wurde
2021
Titel
Chemometric and Transcriptomic Profiling, Microtubule Disruption and Cell Death Induction by Secalonic Acid in Tumor Cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
25

Data source: PubMed

Author's licence
CC-BY
Autoren
  • Nadire Özenver
  • Mona Dawood
  • Edmond Fleischer
  • Anette Klinger
  • Thomas Efferth
Hosting institution
Universitätsbibliothek Mainz
Sammlungen
  • JGU-Publikationen
Resource version
Published version
DOI
10.3390/molecules25143224
Funding acknowledgements
  • DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin Mainz
File(s) embargoed
false
Open access
true
ISSN
1420-3049
Ausgabe der Veröffentlichung
14
Zeitschrift
Molecules
Schlüsselwörter
  • 570 Biowissenschaften
  • 570 Life sciences
Sprache
eng
Open access status
Open Access
Paginierung
3224
Datum der Veröffentlichung
2020
Public URL
https://openscience.ub.uni-mainz.de/handle/20.500.12030/5647
Herausgeber
MDPI
Herausgeber URL
https://doi.org/10.3390/molecules25143224
Datum der Datenerfassung
2021
Datum, an dem der Datensatz öffentlich gemacht wurde
2021
Zugang
Public
Titel
Chemometric and transcriptomic profiling, microtubule disruption and cell death induction by secalonic acid in tumor cells
Ausgabe der Zeitschrift
25

Files

özenver_nadire-chemometric_an-20210209112718237.pdf

Data source: OPENSCIENCE.UB

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