Circulating, cell-free DNA as a marker for exercise load in intermittent sports
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Nils Haller
- Susanne Helmig
- Pascal Taenny
- Julian Petry
- Sebastian Schmidt
- Perikles Simon
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000423416600117&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1371/journal.pone.0191915
- Externe Identifier
- Clarivate Analytics Document Solution ID: FT8QE
- PubMed Identifier: 29370268
- ISSN
- 1932-6203
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- PLOS ONE
- Artikelnummer
- ARTN e0191915
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Titel
- Circulating, cell-free DNA as a marker for exercise load in intermittent sports
- Sub types
- Article
- Ausgabe der Zeitschrift
- 13
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Nils Haller
- Susanne Helmig
- Pascal Taenny
- Julian Petry
- Sebastian Schmidt
- Perikles Simon
- DOI
- 10.1371/journal.pone.0191915
- Editoren
- Alessandro Zagatto
- eISSN
- 1932-6203
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- PLOS ONE
- Sprache
- en
- Online publication date
- 2018
- Paginierung
- e0191915 - e0191915
- Status
- Published online
- Herausgeber
- Public Library of Science (PLoS)
- Herausgeber URL
- http://dx.doi.org/10.1371/journal.pone.0191915
- Datum der Datenerfassung
- 2020
- Titel
- Circulating, cell-free DNA as a marker for exercise load in intermittent sports
- Ausgabe der Zeitschrift
- 13
Data source: Crossref
- Abstract
- <h4>Background</h4>Attempts to establish a biomarker reflecting individual player load in intermittent sports such as football have failed so far. Increases in circulating DNA (cfDNA) have been demonstrated in various endurance sports settings. While it has been proposed that cfDNA could be a suitable marker for player load in intermittent sports, the effects on cfDNA of repeated sprinting as an essential feature in intermittent sports are unknown. For the first time, we assessed both alterations of cfDNA due to repeated maximal sprints and due to a professional football game.<h4>Methods</h4>Nine participants were subjected to a standardised sprint training session with cross-over design of five maximal sprints of 40 meters with either "short" (1 minute) or "long" pauses (5 minutes). Capillary cfDNA and lactate were measured after every sprint and venous cfDNA before and after each series of sprints. Moreover, capillary cfDNA and lactate values were taken in 23 professional football players before and after incremental exercise testing, during the course of a training week at rest (baseline) and in all 17 enrolled players following a season game.<h4>Results</h4>Lactate and venous cfDNA increased more pronounced during "short" compared to "long" (1.4-fold, p = 0.032 and 1.7-fold, p = 0.016) and cfDNA correlated significantly with lactate (r = 0.69; p<0.001). Incremental exercise testing increased cfDNA 7.0-fold (p<0.001). The season game increased cfDNA 22.7-fold (p<0.0001), while lactate showed a 2.0-fold (p = 0.09) increase compared to baseline. Fold-changes in cfDNA correlated with distance covered during game (spearman's r = 0.87, p = 0.0012), while no correlation between lactate and the tracking data could be found.<h4>Discussion</h4>We show for the first time that cfDNA could be an objective marker for distance covered in elite intermittent sports. In contrast to the potential of more established blood-based markers like IL-6, CK, or CRP, cfDNA shows by far the strongest fold-change and a high correlation with a particular load related aspect in professional football.
- Addresses
- Department of Sports Medicine, Rehabilitation and Prevention, Johannes Gutenberg-University of Mainz, Mainz, Germany.
- Autoren
- Nils Haller
- Susanne Helmig
- Pascal Taenny
- Julian Petry
- Sebastian Schmidt
- Perikles Simon
- DOI
- 10.1371/journal.pone.0191915
- eISSN
- 1932-6203
- Externe Identifier
- PubMed Identifier: 29370268
- PubMed Central ID: PMC5784997
- Open access
- true
- ISSN
- 1932-6203
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- PloS one
- Schlüsselwörter
- Humans
- Exercise
- Sports
- Female
- Male
- Biomarkers
- Cell-Free Nucleic Acids
- Sprache
- eng
- Medium
- Electronic-eCollection
- Online publication date
- 2018
- Open access status
- Open Access
- Paginierung
- e0191915
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2018
- Titel
- Circulating, cell-free DNA as a marker for exercise load in intermittent sports.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 13
Files
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0191915&type=printable https://europepmc.org/articles/PMC5784997?pdf=render
Data source: Europe PubMed Central
- Abstract
- BACKGROUND: Attempts to establish a biomarker reflecting individual player load in intermittent sports such as football have failed so far. Increases in circulating DNA (cfDNA) have been demonstrated in various endurance sports settings. While it has been proposed that cfDNA could be a suitable marker for player load in intermittent sports, the effects on cfDNA of repeated sprinting as an essential feature in intermittent sports are unknown. For the first time, we assessed both alterations of cfDNA due to repeated maximal sprints and due to a professional football game. METHODS: Nine participants were subjected to a standardised sprint training session with cross-over design of five maximal sprints of 40 meters with either "short" (1 minute) or "long" pauses (5 minutes). Capillary cfDNA and lactate were measured after every sprint and venous cfDNA before and after each series of sprints. Moreover, capillary cfDNA and lactate values were taken in 23 professional football players before and after incremental exercise testing, during the course of a training week at rest (baseline) and in all 17 enrolled players following a season game. RESULTS: Lactate and venous cfDNA increased more pronounced during "short" compared to "long" (1.4-fold, p = 0.032 and 1.7-fold, p = 0.016) and cfDNA correlated significantly with lactate (r = 0.69; p<0.001). Incremental exercise testing increased cfDNA 7.0-fold (p<0.001). The season game increased cfDNA 22.7-fold (p<0.0001), while lactate showed a 2.0-fold (p = 0.09) increase compared to baseline. Fold-changes in cfDNA correlated with distance covered during game (spearman's r = 0.87, p = 0.0012), while no correlation between lactate and the tracking data could be found. DISCUSSION: We show for the first time that cfDNA could be an objective marker for distance covered in elite intermittent sports. In contrast to the potential of more established blood-based markers like IL-6, CK, or CRP, cfDNA shows by far the strongest fold-change and a high correlation with a particular load related aspect in professional football.
- Date of acceptance
- 2017
- Autoren
- Nils Haller
- Susanne Helmig
- Pascal Taenny
- Julian Petry
- Sebastian Schmidt
- Perikles Simon
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/29370268
- DOI
- 10.1371/journal.pone.0191915
- eISSN
- 1932-6203
- Externe Identifier
- PubMed Central ID: PMC5784997
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- PLoS One
- Schlüsselwörter
- Biomarkers
- Cell-Free Nucleic Acids
- Exercise
- Female
- Humans
- Male
- Sports
- Sprache
- eng
- Country
- United States
- Paginierung
- e0191915
- PII
- PONE-D-17-18381
- Datum der Veröffentlichung
- 2018
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2018
- Titel
- Circulating, cell-free DNA as a marker for exercise load in intermittent sports.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 13
Data source: PubMed
- Author's licence
- CC-BY
- Autoren
- Nils Haller
- Susanne Helmig
- Pascal Taenny
- Julian Petry
- Sebastian Schmidt
- Perikles Simon
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- JGU-Publikationen
- Resource version
- Published version
- DOI
- 10.1371/journal.pone.0191915
- Funding acknowledgements
- DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 1932-6203
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- PLOS ONE
- Schlüsselwörter
- 796 Sport
- 796 Athletic and outdoor sports and games
- Sprache
- eng
- Open access status
- Open Access
- Paginierung
- e0191915
- Datum der Veröffentlichung
- 2018
- Public URL
- https://openscience.ub.uni-mainz.de/handle/20.500.12030/381
- Herausgeber
- PLOS
- Herausgeber URL
- http://dx.doi.org/10.1371/journal.pone.0191915
- Datum der Datenerfassung
- 2018
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2018
- Zugang
- Public
- Titel
- Circulating, cell-free DNA as a marker for exercise load in intermittent sports
- Ausgabe der Zeitschrift
- 13
Files
58192.pdf
Data source: OPENSCIENCE.UB
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