Medium chain acylcarnitines dominate the metabolite pattern in humans under moderate intensity exercise and support lipid oxidation
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Rainer Lehmann
- Xinjie Zhao
- Cora Weigert
- Perikles Simon
- Elvira Fehrenbach
- Jens Fritsche
- Jurgen Machann
- Fritz Schick
- Jiangshan Wang
- Miriam Hoene
- Erwin D Schleicher
- Hans-Ulrich Haring
- Guowang Xu
- Andreas M Niess
- Sammlungen
- metadata
- ISSN
- 1932-6203
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- PLoS one
- Schlüsselwörter
- 796 Sport
- 796 Athletic and outdoor sports and games
- Sprache
- eng
- Datum der Veröffentlichung
- 2010
- Herausgeber
- PLoS
- Herausgeber URL
- http://dx.doi.org/10.1371/journal.pone.0011519
- Datum der Datenerfassung
- 2020
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Zugang
- Public
- Titel
- Medium chain acylcarnitines dominate the metabolite pattern in humans under moderate intensity exercise and support lipid oxidation
- Ausgabe der Zeitschrift
- 5
Datenquelle: METADATA.UB
- Andere Metadatenquellen:
-
- Autoren
- Rainer Lehmann
- Xinjie Zhao
- Cora Weigert
- Perikles Simon
- Elvira Fehrenbach
- Jens Fritsche
- Juergen Machann
- Fritz Schick
- Jiangshan Wang
- Miriam Hoene
- Erwin D Schleicher
- Hans-Ulrich Haering
- Guowang Xu
- Andreas M Niess
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000279781600007&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1371/journal.pone.0011519
- Externe Identifier
- Clarivate Analytics Document Solution ID: 623XV
- PubMed Identifier: 20634953
- ISSN
- 1932-6203
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- PLOS ONE
- Artikelnummer
- ARTN e11519
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Titel
- Medium Chain Acylcarnitines Dominate the Metabolite Pattern in Humans under Moderate Intensity Exercise and Support Lipid Oxidation
- Sub types
- Article
- Ausgabe der Zeitschrift
- 5
Datenquelle: Web of Science (Lite)
- Autoren
- Rainer Lehmann
- Xinjie Zhao
- Cora Weigert
- Perikles Simon
- Elvira Fehrenbach
- Jens Fritsche
- Jürgen Machann
- Fritz Schick
- Jiangshan Wang
- Miriam Hoene
- Erwin D Schleicher
- Hans-Ulrich Häring
- Guowang Xu
- Andreas M Niess
- DOI
- 10.1371/journal.pone.0011519
- Editoren
- Mark A Tarnopolsky
- eISSN
- 1932-6203
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- PLoS ONE
- Sprache
- en
- Online publication date
- 2010
- Paginierung
- e11519 - e11519
- Status
- Published online
- Herausgeber
- Public Library of Science (PLoS)
- Herausgeber URL
- http://dx.doi.org/10.1371/journal.pone.0011519
- Datum der Datenerfassung
- 2021
- Titel
- Medium Chain Acylcarnitines Dominate the Metabolite Pattern in Humans under Moderate Intensity Exercise and Support Lipid Oxidation
- Ausgabe der Zeitschrift
- 5
Datenquelle: Crossref
- Abstract
- <h4>Background</h4>Exercise is an extreme physiological challenge for skeletal muscle energy metabolism and has notable health benefits. We aimed to identify and characterize metabolites, which are components of the regulatory network mediating the beneficial metabolic adaptation to exercise.<h4>Methodology and principal findings</h4>First, we investigated plasma from healthy human subjects who completed two independent running studies under moderate, predominantly aerobic conditions. Samples obtained prior to and immediately after running and then 3 and 24 h into the recovery phase were analyzed by a non-targeted (NT-) metabolomics approach applying liquid chromatography-qTOF-mass spectrometry. Under these conditions medium and long chain acylcarnitines were found to be the most discriminant plasma biomarkers of moderately intense exercise. Immediately after a 60 min (at 93% V(IAT)) or a 120 min run (at 70% V(IAT)) a pronounced, transient increase dominated by octanoyl-, decanoyl-, and dodecanoyl-carnitine was observed. The release of acylcarnitines as intermediates of partial beta-oxidation was verified in skeletal muscle cell culture experiments by probing (13)C-palmitate metabolism. Further investigations in primary human myotubes and mouse muscle tissue revealed that octanoyl-, decanoyl-, and dodecanoyl-carnitine were able to support the oxidation of palmitate, proving more effective than L-carnitine.<h4>Conclusions</h4>Medium chain acylcarnitines were identified and characterized by a functional metabolomics approach as the dominating biomarkers during a moderately intense exercise bout possessing the power to support fat oxidation. This physiological production and efflux of acylcarnitines might exert beneficial biological functions in muscle tissue.
- Addresses
- Central Laboratory, Division of Clinical Chemistry and Pathobiochemistry, University Hospital Tuebingen, Tuebingen, Germany. Rainer.Lehmann@med.uni-tuebingen.de
- Autoren
- Rainer Lehmann
- Xinjie Zhao
- Cora Weigert
- Perikles Simon
- Elvira Fehrenbach
- Jens Fritsche
- Jürgen Machann
- Fritz Schick
- Jiangshan Wang
- Miriam Hoene
- Erwin D Schleicher
- Hans-Ulrich Häring
- Guowang Xu
- Andreas M Niess
- DOI
- 10.1371/journal.pone.0011519
- eISSN
- 1932-6203
- Externe Identifier
- PubMed Identifier: 20634953
- PubMed Central ID: PMC2902514
- Open access
- true
- ISSN
- 1932-6203
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- PloS one
- Schlüsselwörter
- Muscle, Skeletal
- Cells, Cultured
- Animals
- Humans
- Mice
- Carnitine
- Exercise
- Chromatography, High Pressure Liquid
- Magnetic Resonance Spectroscopy
- Reverse Transcriptase Polymerase Chain Reaction
- Oxidation-Reduction
- Adult
- Male
- Lipid Metabolism
- Mass Spectrometry
- Muscle Fibers, Skeletal
- In Vitro Techniques
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2010
- Open access status
- Open Access
- Paginierung
- e11519
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2010
- Titel
- Medium chain acylcarnitines dominate the metabolite pattern in humans under moderate intensity exercise and support lipid oxidation.
- Sub types
- Clinical Trial
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 5
Files
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0011519&type=printable https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20634953/pdf/?tool=EBI https://europepmc.org/articles/PMC2902514?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- BACKGROUND: Exercise is an extreme physiological challenge for skeletal muscle energy metabolism and has notable health benefits. We aimed to identify and characterize metabolites, which are components of the regulatory network mediating the beneficial metabolic adaptation to exercise. METHODOLOGY AND PRINCIPAL FINDINGS: First, we investigated plasma from healthy human subjects who completed two independent running studies under moderate, predominantly aerobic conditions. Samples obtained prior to and immediately after running and then 3 and 24 h into the recovery phase were analyzed by a non-targeted (NT-) metabolomics approach applying liquid chromatography-qTOF-mass spectrometry. Under these conditions medium and long chain acylcarnitines were found to be the most discriminant plasma biomarkers of moderately intense exercise. Immediately after a 60 min (at 93% V(IAT)) or a 120 min run (at 70% V(IAT)) a pronounced, transient increase dominated by octanoyl-, decanoyl-, and dodecanoyl-carnitine was observed. The release of acylcarnitines as intermediates of partial beta-oxidation was verified in skeletal muscle cell culture experiments by probing (13)C-palmitate metabolism. Further investigations in primary human myotubes and mouse muscle tissue revealed that octanoyl-, decanoyl-, and dodecanoyl-carnitine were able to support the oxidation of palmitate, proving more effective than L-carnitine. CONCLUSIONS: Medium chain acylcarnitines were identified and characterized by a functional metabolomics approach as the dominating biomarkers during a moderately intense exercise bout possessing the power to support fat oxidation. This physiological production and efflux of acylcarnitines might exert beneficial biological functions in muscle tissue.
- Date of acceptance
- 2010
- Autoren
- Rainer Lehmann
- Xinjie Zhao
- Cora Weigert
- Perikles Simon
- Elvira Fehrenbach
- Jens Fritsche
- Jürgen Machann
- Fritz Schick
- Jiangshan Wang
- Miriam Hoene
- Erwin D Schleicher
- Hans-Ulrich Häring
- Guowang Xu
- Andreas M Niess
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/20634953
- DOI
- 10.1371/journal.pone.0011519
- eISSN
- 1932-6203
- Externe Identifier
- PubMed Central ID: PMC2902514
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- PLoS One
- Schlüsselwörter
- Adult
- Animals
- Carnitine
- Cells, Cultured
- Chromatography, High Pressure Liquid
- Exercise
- Humans
- In Vitro Techniques
- Lipid Metabolism
- Magnetic Resonance Spectroscopy
- Male
- Mass Spectrometry
- Mice
- Muscle Fibers, Skeletal
- Muscle, Skeletal
- Oxidation-Reduction
- Reverse Transcriptase Polymerase Chain Reaction
- Sprache
- eng
- Country
- United States
- Paginierung
- e11519
- Datum der Veröffentlichung
- 2010
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2010
- Titel
- Medium chain acylcarnitines dominate the metabolite pattern in humans under moderate intensity exercise and support lipid oxidation.
- Sub types
- Clinical Trial
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 5
Datenquelle: PubMed
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