EGR-1 is regulated by N-methyl-D-aspartate-receptor stimulation and associated with patient survival in human high grade astrocytomas
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Michel Mittelbronn
- Patrick Harter
- Arne Warth
- Adrian Lupescu
- Karin Schilbach
- Henning Vollmann
- David Capper
- Benjamin Goeppert
- Karl Frei
- Helmut Bertalanffy
- Michael Weller
- Richard Meyermann
- Florian Lang
- Perikles Simon
- Sammlungen
- metadata
- ISSN
- 1015-6305
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Brain pathology
- Schlüsselwörter
- 796 Sport
- 796 Athletic and outdoor sports and games
- Sprache
- eng
- Paginierung
- Seiten: 195 - 204
- Datum der Veröffentlichung
- 2009
- Herausgeber URL
- http://dx.doi.org/10.1111/j.1750-3639.2008.00175.x
- Datum der Datenerfassung
- 2020
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Zugang
- Public
- Titel
- EGR-1 is regulated by N-methyl-D-aspartate-receptor stimulation and associated with patient survival in human high grade astrocytomas
- Ausgabe der Zeitschrift
- 19
Datenquelle: METADATA.UB
- Andere Metadatenquellen:
-
- Autoren
- Michel Mittelbronn
- Patrick Harter
- Arne Warth
- Adrian Lupescu
- Karin Schilbach
- Henning Vollmann
- David Capper
- Benjamin Goeppert
- Karl Frei
- Helmut Bertalanffy
- Michael Weller
- Richard Meyermann
- Florian Lang
- Perikles Simon
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000263909100003&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1111/j.1750-3639.2008.00175.x
- Externe Identifier
- Clarivate Analytics Document Solution ID: 415BK
- PubMed Identifier: 18489490
- ISSN
- 1015-6305
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- BRAIN PATHOLOGY
- Schlüsselwörter
- Astrocytomas
- EGR-1
- NMDA-R
- Paginierung
- 195 - 204
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Titel
- EGR-1 is Regulated by N-Methyl-D-Aspartate-Receptor Stimulation and Associated with Patient Survival in Human High Grade Astrocytomas
- Sub types
- Article
- Ausgabe der Zeitschrift
- 19
Datenquelle: Web of Science (Lite)
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Early growth response‐1 (EGR‐1) is considered a central regulator in tumor cell proliferation, migration and angiogenesis and a promising candidate for gene therapy in human astrocytomas. However, conflicting data have been reported suggesting that both tumor promoting and anti‐tumor activity of EGR‐1 and its regulation, expression and prognostic significance still remain enigmatic. Our study explored EGR‐1 expression and regulation in astrocytomas and its association with patient survival. As we detected two EGR‐1 mRNA variants, one containing a N‐methyl‐D‐aspartate‐receptor (NMDA‐R) responsive cytoplasmic polyadenylation element (CPE), further experiments were performed to determine the functional role of this pathway. After NMDA stimulation of SV‐FHAS and neoplastic astrocytes, real‐time polymerase chain reaction showed an increase of the CPE, containing EGR‐1 splice variant only in astrocytoma cells. The surface expression and functionality of NMDA‐R were demonstrated by flow cytometric analysis and measurement of increased intracellular Ca<jats:sup>2+</jats:sup>. EGR‐1 was mainly restricted to tumor cells expressing NMDA‐R and significantly up‐regulated in astrocytic tumors compared with normal brain. Further, EGR‐1 expression was significantly (<jats:italic>P</jats:italic> < 0.007) associated with enhanced patient survival and was an independent prognostic factor in multivariate analysis in high grade astrocytomas. The NMDA‐R‐mediated EGR‐1 expression, therefore, seems to be a promising target for novel clinical approaches to astrocytoma treatment.</jats:p>
- Autoren
- Michel Mittelbronn
- Patrick Harter
- Arne Warth
- Adrian Lupescu
- Karin Schilbach
- Henning Vollmann
- David Capper
- Benjamin Goeppert
- Karl Frei
- Helmut Bertalanffy
- Michael Weller
- Richard Meyermann
- Florian Lang
- Perikles Simon
- DOI
- 10.1111/j.1750-3639.2008.00175.x
- eISSN
- 1750-3639
- ISSN
- 1015-6305
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Brain Pathology
- Sprache
- en
- Online publication date
- 2009
- Paginierung
- 195 - 204
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1111/j.1750-3639.2008.00175.x
- Datum der Datenerfassung
- 2023
- Titel
- EGR‐1 is Regulated by N‐Methyl‐D‐Aspartate‐Receptor Stimulation and Associated with Patient Survival in Human High Grade Astrocytomas
- Ausgabe der Zeitschrift
- 19
Datenquelle: Crossref
- Abstract
- Early growth response-1 (EGR-1) is considered a central regulator in tumor cell proliferation, migration and angiogenesis and a promising candidate for gene therapy in human astrocytomas. However, conflicting data have been reported suggesting that both tumor promoting and anti-tumor activity of EGR-1 and its regulation, expression and prognostic significance still remain enigmatic. Our study explored EGR-1 expression and regulation in astrocytomas and its association with patient survival. As we detected two EGR-1 mRNA variants, one containing a N-methyl-D-aspartate-receptor (NMDA-R) responsive cytoplasmic polyadenylation element (CPE), further experiments were performed to determine the functional role of this pathway. After NMDA stimulation of SV-FHAS and neoplastic astrocytes, real-time polymerase chain reaction showed an increase of the CPE, containing EGR-1 splice variant only in astrocytoma cells. The surface expression and functionality of NMDA-R were demonstrated by flow cytometric analysis and measurement of increased intracellular Ca(2+). EGR-1 was mainly restricted to tumor cells expressing NMDA-R and significantly up-regulated in astrocytic tumors compared with normal brain. Further, EGR-1 expression was significantly (P < 0.007) associated with enhanced patient survival and was an independent prognostic factor in multivariate analysis in high grade astrocytomas. The NMDA-R-mediated EGR-1 expression, therefore, seems to be a promising target for novel clinical approaches to astrocytoma treatment.
- Addresses
- Institute of Brain Research, University of Tübingen, Tübingen, Germany. mittelbronn@usz.ch
- Autoren
- Michel Mittelbronn
- Patrick Harter
- Arne Warth
- Adrian Lupescu
- Karin Schilbach
- Henning Vollmann
- David Capper
- Benjamin Goeppert
- Karl Frei
- Helmut Bertalanffy
- Michael Weller
- Richard Meyermann
- Florian Lang
- Perikles Simon
- DOI
- 10.1111/j.1750-3639.2008.00175.x
- eISSN
- 1750-3639
- Externe Identifier
- PubMed Identifier: 18489490
- PubMed Central ID: PMC8094652
- Open access
- false
- ISSN
- 1015-6305
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Brain pathology (Zurich, Switzerland)
- Schlüsselwörter
- Brain
- Cell Line, Tumor
- Tumor Cells, Cultured
- Humans
- Astrocytoma
- Glioblastoma
- Brain Neoplasms
- Calcium
- N-Methylaspartate
- Receptors, N-Methyl-D-Aspartate
- Protein Isoforms
- RNA, Messenger
- Fluorescent Antibody Technique
- Flow Cytometry
- Immunohistochemistry
- Multivariate Analysis
- Polymerase Chain Reaction
- Gene Expression Regulation, Neoplastic
- Early Growth Response Protein 1
- Kaplan-Meier Estimate
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2008
- Paginierung
- 195 - 204
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Datum der Datenerfassung
- 2008
- Titel
- EGR-1 is regulated by N-methyl-D-aspartate-receptor stimulation and associated with patient survival in human high grade astrocytomas.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 19
Files
https://europepmc.org/articles/PMC8094652?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Early growth response-1 (EGR-1) is considered a central regulator in tumor cell proliferation, migration and angiogenesis and a promising candidate for gene therapy in human astrocytomas. However, conflicting data have been reported suggesting that both tumor promoting and anti-tumor activity of EGR-1 and its regulation, expression and prognostic significance still remain enigmatic. Our study explored EGR-1 expression and regulation in astrocytomas and its association with patient survival. As we detected two EGR-1 mRNA variants, one containing a N-methyl-D-aspartate-receptor (NMDA-R) responsive cytoplasmic polyadenylation element (CPE), further experiments were performed to determine the functional role of this pathway. After NMDA stimulation of SV-FHAS and neoplastic astrocytes, real-time polymerase chain reaction showed an increase of the CPE, containing EGR-1 splice variant only in astrocytoma cells. The surface expression and functionality of NMDA-R were demonstrated by flow cytometric analysis and measurement of increased intracellular Ca(2+). EGR-1 was mainly restricted to tumor cells expressing NMDA-R and significantly up-regulated in astrocytic tumors compared with normal brain. Further, EGR-1 expression was significantly (P < 0.007) associated with enhanced patient survival and was an independent prognostic factor in multivariate analysis in high grade astrocytomas. The NMDA-R-mediated EGR-1 expression, therefore, seems to be a promising target for novel clinical approaches to astrocytoma treatment.
- Autoren
- Michel Mittelbronn
- Patrick Harter
- Arne Warth
- Adrian Lupescu
- Karin Schilbach
- Henning Vollmann
- David Capper
- Benjamin Goeppert
- Karl Frei
- Helmut Bertalanffy
- Michael Weller
- Richard Meyermann
- Florian Lang
- Perikles Simon
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/18489490
- DOI
- 10.1111/j.1750-3639.2008.00175.x
- eISSN
- 1750-3639
- Externe Identifier
- PubMed Central ID: PMC8094652
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Brain Pathol
- Schlüsselwörter
- Astrocytoma
- Brain
- Brain Neoplasms
- Calcium
- Cell Line, Tumor
- Early Growth Response Protein 1
- Flow Cytometry
- Fluorescent Antibody Technique
- Gene Expression Regulation, Neoplastic
- Glioblastoma
- Humans
- Immunohistochemistry
- Kaplan-Meier Estimate
- Multivariate Analysis
- N-Methylaspartate
- Polymerase Chain Reaction
- Protein Isoforms
- RNA, Messenger
- Receptors, N-Methyl-D-Aspartate
- Tumor Cells, Cultured
- Sprache
- eng
- Country
- Switzerland
- Paginierung
- 195 - 204
- PII
- BPA175
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2009
- Titel
- EGR-1 is regulated by N-methyl-D-aspartate-receptor stimulation and associated with patient survival in human high grade astrocytomas.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 19
Datenquelle: PubMed
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