Circulating cell-free DNA as a biomarker for injury prediction?
- Publikationstyp:
- Konferenzbeitrag
- Metadaten:
-
- Abstract
- INTRODUCTION: Circulating cell-free DNA (cfDNA) is considered an emerging biomarker in exercise physiology. Increases during exercise depend on exercise duration and intensity [1]. Changes in cfDNA concentrations could be associated with the onset of the overtraining syndrome [2]. This case study (n=2) investigates a possible association between cfDNA levels and the incidence of overload, which prevented one participant from completing the Salzburg 10/7 HIIT study (S10/7, NCT05067426) compared to one subject who completed the study. METHODS: S10/7 investigates the effects of a 7-day (d) high-intensity interval training (HIIT) shock microcycle. The study consists of a baseline period (b) of 9 d with time points T0 (d 1 b) and T1 (d 8 b), an intervention period of 7 d (10 HIITs: 5x4 min at 90-95% HRmax with 2.5 min rest in between, followed by a 30-min run at low intensity below the lactate threshold (LT) of 1.5 mmol/l) with time points T2 (d 2) and T3 (d 7) and a post-intervention phase of 14 d with physiological exercise testing (PET) on three time points. Case 1 (C1; m, 23 yrs., 190 cm, 87.2 kg, LT 12.5 km/h) and -2 (C2; m, 30 yrs., 166 cm, 63.5 kg, LT 14 km/h) were analyzed. Participants complete baseline measurements (blood parameters, ECG, questionnaires) at T0 and a PET at T1. At T2 and T3, participants complete two supervised HIITs in the lab, one in the morning (AM) and the second one in the afternoon (PM). Venous cfDNA levels before and immediately after exercise and chronic changes of cytokine levels at rest were analyzed in C1 and C2. RESULTS: C1 completed the study, whereas C2 discontinued the study on d 4 of the intervention due to iliotibial band syndrome during the intervention phase. Mean cfDNA levels (in ng/ml) were 6.8 (C1) and 7.4 (C2) before and 60.4 (C1) and 220.2 (C2) after PET at T1, with foldchanges (FC) of 8.9 (C1) and 29.6 (C2), respectively. Mean cfDNA levels were 6.7 (C1) and 5.8 (C2) before and 82.5 (C1) and 300.1 (C2) after HIITs at T2, with FC of 12.6 (C1) and 51.7 (C2). IL2 levels (in pg/ml) were 2.1 (C1) and 9.6 (C2) at T0, 2.7 (C1) and 10.7 (C2) at T1 2.4 (C1) and 11.7 (C2) at T2 AM. IL6 levels were 21.6 (C1) and 75.2 (C2) at T0, 26.1 (C1) and 87.7 (C2) at T1, 23.6 (C1) and 91.5 (C2) at T2 AM. CONCLUSION: Absolute concentrations and changes in both cfDNA and inflammatory cytokines were more pronounced in C2, who discontinued the study because of an injury. In another study with 13 participants of almost similar aerobic fitness, PET provoked on average FC of 7.4, only [1]. Future studies need to systematically analyze larger injury-specific sample to clarify if cfDNA could be a biomarker for predicting overload and injury.
- Date of acceptance
- 2022
- Addresses
- Aleksandar Tomaskovic
- Autoren
- Aleksandar Tomaskovic
- Thomas Stoeggl
- Julia Blumkaitis
- Tilmann Strepp
- Lorenz Aglas
- Mario Wenger
- Elmo Neuberger
- Perikles Simon
- Nils Haller
- Autoren-URL
- https://www.sportmedizin.uni-mainz.de/ueber-uns/team/profil-aleksandar-tomaskovic/
- Editoren
- F Dela
- MF Piacentini
- JW Helge
- Á Calvo LIuch
- E Sáez
- F Pareja Blanco
- E Tsolakidis
- Conference finish date
- 2022
- Funding acknowledgements
- The study is a cooperation project between the University of Salzburg, the University of Mainz and the Red Bull Athlete Performance Center. The study receives funding from the Red Bull Athlete Performance Center for the scientifc accompaniment of the monitoring concept in the context of which data for the current study were collected.
- ISBN-13
- 978-3-9818414-5-9
- Ausgabe der Veröffentlichung
- 27
- Zeitschrift
- 27th Annual Congress of the European College of Sport Science Book of Abstracts
- Schlüsselwörter
- cfDNA, biomarker, sports medicine, diagnostics, injury prevention
- Conference place
- Sevilla, España
- Medium
- Digital and printed book
- Name of conference
- 27th Annual Congress of the European College of Sport Science
- Online publication date
- 2022
- Open access status
- Open Access
- Paginierung
- 158 - 158, 1
- Ort der Veröffentlichung
- Cologne, Germany
- Datum der Veröffentlichung
- 2022
- Status
- Published
- Herausgeber
- Conception, DTP: SporTools GmbH – Data management in sports
- Publisher licence
- Unknown
- Herausgeber URL
- https://www.sport-science.org/index.php/sevilla-2022-home
- Datum der Datenerfassung
- 2022
- Conference start date
- 2022
- Titel
- Circulating cell-free DNA as a biomarker for injury prediction?
- Ausgabe der Zeitschrift
- 27
Datenquelle: Manual
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