Circulating cell-free DNA as a biomarker for injury prediction?

Publikationstyp:

Konferenzbeitrag

Metadaten:

Abstract

INTRODUCTION: Circulating cell-free DNA (cfDNA) is considered an emerging biomarker in exercise physiology. Increases during exercise depend on exercise duration and intensity [1]. Changes in cfDNA concentrations could be associated with the onset of the overtraining syndrome [2]. This case study (n=2) investigates a possible association between cfDNA levels and the incidence of overload, which prevented one participant from completing the Salzburg 10/7 HIIT study (S10/7, NCT05067426) compared to one subject who completed the study. METHODS: S10/7 investigates the effects of a 7-day (d) high-intensity interval training (HIIT) shock microcycle. The study consists of a baseline period (b) of 9 d with time points T0 (d 1 b) and T1 (d 8 b), an intervention period of 7 d (10 HIITs: 5x4 min at 90-95% HRmax with 2.5 min rest in between, followed by a 30-min run at low intensity below the lactate threshold (LT) of 1.5 mmol/l) with time points T2 (d 2) and T3 (d 7) and a post-intervention phase of 14 d with physiological exercise testing (PET) on three time points. Case 1 (C1; m, 23 yrs., 190 cm, 87.2 kg, LT 12.5 km/h) and -2 (C2; m, 30 yrs., 166 cm, 63.5 kg, LT 14 km/h) were analyzed. Participants complete baseline measurements (blood parameters, ECG, questionnaires) at T0 and a PET at T1. At T2 and T3, participants complete two supervised HIITs in the lab, one in the morning (AM) and the second one in the afternoon (PM). Venous cfDNA levels before and immediately after exercise and chronic changes of cytokine levels at rest were analyzed in C1 and C2. RESULTS: C1 completed the study, whereas C2 discontinued the study on d 4 of the intervention due to iliotibial band syndrome during the intervention phase. Mean cfDNA levels (in ng/ml) were 6.8 (C1) and 7.4 (C2) before and 60.4 (C1) and 220.2 (C2) after PET at T1, with foldchanges (FC) of 8.9 (C1) and 29.6 (C2), respectively. Mean cfDNA levels were 6.7 (C1) and 5.8 (C2) before and 82.5 (C1) and 300.1 (C2) after HIITs at T2, with FC of 12.6 (C1) and 51.7 (C2). IL2 levels (in pg/ml) were 2.1 (C1) and 9.6 (C2) at T0, 2.7 (C1) and 10.7 (C2) at T1 2.4 (C1) and 11.7 (C2) at T2 AM. IL6 levels were 21.6 (C1) and 75.2 (C2) at T0, 26.1 (C1) and 87.7 (C2) at T1, 23.6 (C1) and 91.5 (C2) at T2 AM. CONCLUSION: Absolute concentrations and changes in both cfDNA and inflammatory cytokines were more pronounced in C2, who discontinued the study because of an injury. In another study with 13 participants of almost similar aerobic fitness, PET provoked on average FC of 7.4, only [1]. Future studies need to systematically analyze larger injury-specific sample to clarify if cfDNA could be a biomarker for predicting overload and injury.

Date of acceptance

2022

Addresses

Aleksandar Tomaskovic

Autoren

Aleksandar Tomaskovic
Thomas Stoeggl
Julia Blumkaitis
Tilmann Strepp
Lorenz Aglas
Mario Wenger
Elmo Neuberger
Perikles Simon
Nils Haller

Autoren-URL

https://www.sportmedizin.uni-mainz.de/ueber-uns/team/profil-aleksandar-tomaskovic/

Editoren

F Dela
MF Piacentini
JW Helge
Á Calvo LIuch
E Sáez
F Pareja Blanco
E Tsolakidis

Conference finish date

2022

Funding acknowledgements

The study is a cooperation project between the University of Salzburg, the University of Mainz and the Red Bull Athlete Performance Center. The study receives funding from the Red Bull Athlete Performance Center for the scientifc accompaniment of the monitoring concept in the context of which data for the current study were collected.

ISBN-13

978-3-9818414-5-9

Ausgabe der Veröffentlichung

Zeitschrift

27th Annual Congress of the European College of Sport Science Book of Abstracts

Schlüsselwörter

cfDNA, biomarker, sports medicine, diagnostics, injury prevention

Conference place

Sevilla, España

Medium

Digital and printed book

Name of conference

27th Annual Congress of the European College of Sport Science

Online publication date

2022

Open access status

Open Access

Paginierung

158 - 158, 1

Ort der Veröffentlichung

Cologne, Germany

Datum der Veröffentlichung

2022

Status

Published

Herausgeber

Conception, DTP: SporTools GmbH – Data management in sports

Publisher licence

Unknown

Herausgeber URL

https://www.sport-science.org/index.php/sevilla-2022-home

Datum der Datenerfassung