Cytotoxicity, mode of action and antibacterial activities of selected Saudi Arabian medicinal plants
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Victor Kuete
- Benjamin Wiench
- Mansour S Alsaid
- Muhammad A Alyahya
- Aime G Fankam
- Abdelaaty A Shahat
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000328380100001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1186/1472-6882-13-354
- Externe Identifier
- Clarivate Analytics Document Solution ID: 271GP
- PubMed Identifier: 24330397
- ISSN
- 1472-6882
- Zeitschrift
- BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
- Schlüsselwörter
- Antibacterial
- Cytotoxicity
- Mode of action
- Medicinal plants
- Saudi Arabia
- Artikelnummer
- ARTN 354
- Datum der Veröffentlichung
- 2013
- Status
- Published
- Titel
- Cytotoxicity, mode of action and antibacterial activities of selected Saudi Arabian medicinal plants
- Sub types
- Article
- Ausgabe der Zeitschrift
- 13
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>The flora of Saudi Arabia is one of the richest biodiversity areas in the Arabian Peninsula and comprises very important genetic resources of crop and medicinal plants. The present study was designed to investigate the cytotoxicity and the antibacterial activities of the organic extracts from twenty six Saudi Arabian medicinal plants. The study was also extended to the investigation of the effects of the extracts from the four best plants, <jats:italic>Ononis serrata</jats:italic> (SY160), <jats:italic>Haplophyllum tuberculatum</jats:italic> (SY177), <jats:italic>Pulicaria crispa</jats:italic> (SY179), and <jats:italic>Achillea beiberstenii</jats:italic> (SY-200) on cell cycle distribution, apoptosis, caspases activities and mitochondrial function in leukemia CCRF-CEM cell line.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>A resazurin assay was used to assess the cytotoxicity of the extracts on a panel of human cancer cell lines whilst the microbroth dilution was used to determine the minimal inhibitory concentration (MIC) of the samples against twelve bacterial strains belonging to four species, <jats:italic>Escherichia coli</jats:italic>, <jats:italic>Enterobacter aerogenes</jats:italic>, <jats:italic>Klebsiella pneumoniae</jats:italic> and <jats:italic>Pseudomonas aeruginosa</jats:italic>.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>The best activity on leukemia cell lines were recorded with SY177 (IC<jats:sub>50</jats:sub> of 9.94 μg/mL) and SY179 (IC<jats:sub>50</jats:sub> of 1.81 μg/mL) against CCRF-CEM as well as Ach-b (IC<jats:sub>50</jats:sub> of 9.30 μg/mL) and SY160 (IC<jats:sub>50</jats:sub> of 5.06 μg/mL) against HL60 cells. The extracts from SY177 and SY179 were also toxic against the seven solid cancer cell lines studied with the highest IC<jats:sub>50</jats:sub> values of 31.64 μg/mL (SY177 against Hep-G2 cells). SY177 and Ach-b induced cell cycle arrest in G0/G1 and S phases whilst SY160 and SY179 induced arrest in G0/G1 phase. All the four plant extracts induced apoptosis in CCRF-CEM cells with the alteration of the mitochondrial membrane potential. In the antibacterial assays, only Ach-b displayed moderate antibacterial activities against <jats:italic>E. coli</jats:italic> and <jats:italic>E. aerogenes</jats:italic> ATCC strains (MIC of 256 μg/mL), AG100A<jats:sub>TeT</jats:sub> and <jats:italic>K. pneumoniae</jats:italic> ATCC strains (MIC of 128 μg/mL).</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusions</jats:title> <jats:p>Finally, the results of the present investigation provided supportive data for the possible use of some Saudi Arabian plants investigated herein, and mostly <jats:italic>Haplophyllum tuberculatum, Pulicaria crispa</jats:italic>, <jats:italic>Ononis serrata</jats:italic> and <jats:italic>Achillea beiberstenii</jats:italic> in the control of cancer diseases.</jats:p> </jats:sec>
- Autoren
- Victor Kuete
- Benjamin Wiench
- Mansour S Alsaid
- Muhammad A Alyahya
- Aimé G Fankam
- Abdelaaty A Shahat
- Thomas Efferth
- DOI
- 10.1186/1472-6882-13-354
- eISSN
- 1472-6882
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- BMC Complementary and Alternative Medicine
- Sprache
- en
- Artikelnummer
- 354
- Online publication date
- 2013
- Datum der Veröffentlichung
- 2013
- Status
- Published
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1186/1472-6882-13-354
- Datum der Datenerfassung
- 2021
- Titel
- Cytotoxicity, mode of action and antibacterial activities of selected Saudi Arabian medicinal plants
- Ausgabe der Zeitschrift
- 13
Datenquelle: Crossref
- Abstract
- <h4>Background</h4>The flora of Saudi Arabia is one of the richest biodiversity areas in the Arabian Peninsula and comprises very important genetic resources of crop and medicinal plants. The present study was designed to investigate the cytotoxicity and the antibacterial activities of the organic extracts from twenty six Saudi Arabian medicinal plants. The study was also extended to the investigation of the effects of the extracts from the four best plants, Ononis serrata (SY160), Haplophyllum tuberculatum (SY177), Pulicaria crispa (SY179), and Achillea beiberstenii (SY-200) on cell cycle distribution, apoptosis, caspases activities and mitochondrial function in leukemia CCRF-CEM cell line.<h4>Methods</h4>A resazurin assay was used to assess the cytotoxicity of the extracts on a panel of human cancer cell lines whilst the microbroth dilution was used to determine the minimal inhibitory concentration (MIC) of the samples against twelve bacterial strains belonging to four species, Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae and Pseudomonas aeruginosa.<h4>Results</h4>The best activity on leukemia cell lines were recorded with SY177 (IC50 of 9.94 μg/mL) and SY179 (IC50 of 1.81 μg/mL) against CCRF-CEM as well as Ach-b (IC50 of 9.30 μg/mL) and SY160 (IC50 of 5.06 μg/mL) against HL60 cells. The extracts from SY177 and SY179 were also toxic against the seven solid cancer cell lines studied with the highest IC50 values of 31.64 μg/mL (SY177 against Hep-G2 cells). SY177 and Ach-b induced cell cycle arrest in G0/G1 and S phases whilst SY160 and SY179 induced arrest in G0/G1 phase. All the four plant extracts induced apoptosis in CCRF-CEM cells with the alteration of the mitochondrial membrane potential. In the antibacterial assays, only Ach-b displayed moderate antibacterial activities against E. coli and E. aerogenes ATCC strains (MIC of 256 μg/mL), AG100A(TeT) and K. pneumoniae ATCC strains (MIC of 128 μg/mL).<h4>Conclusions</h4>Finally, the results of the present investigation provided supportive data for the possible use of some Saudi Arabian plants investigated herein, and mostly Haplophyllum tuberculatum, Pulicaria crispa, Ononis serrata and Achillea beiberstenii in the control of cancer diseases.
- Autoren
- Victor Kuete
- Benjamin Wiench
- Mansour S Alsaid
- Muhammad A Alyahya
- Aimé G Fankam
- Abdelaaty A Shahat
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1186/1472-6882-13-354
- eISSN
- 1472-6882
- Externe Identifier
- PubMed Identifier: 24330397
- PubMed Central ID: PMC4029389
- Open access
- true
- ISSN
- 1472-6882
- Zeitschrift
- BMC complementary and alternative medicine
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Pseudomonas
- Enterobacteriaceae
- Plants, Medicinal
- Caspases
- Plant Extracts
- Antineoplastic Agents
- Anti-Bacterial Agents
- Microbial Sensitivity Tests
- Cell Cycle
- Apoptosis
- Cell Survival
- Membrane Potential, Mitochondrial
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2013
- Open access status
- Open Access
- Paginierung
- 354
- Datum der Veröffentlichung
- 2013
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2013
- Titel
- Cytotoxicity, mode of action and antibacterial activities of selected Saudi Arabian medicinal plants.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 13
Files
https://bmccomplementmedtherapies.biomedcentral.com/counter/pdf/10.1186/1472-6882-13-354 https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24330397/pdf/?tool=EBI https://europepmc.org/articles/PMC4029389?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- BACKGROUND: The flora of Saudi Arabia is one of the richest biodiversity areas in the Arabian Peninsula and comprises very important genetic resources of crop and medicinal plants. The present study was designed to investigate the cytotoxicity and the antibacterial activities of the organic extracts from twenty six Saudi Arabian medicinal plants. The study was also extended to the investigation of the effects of the extracts from the four best plants, Ononis serrata (SY160), Haplophyllum tuberculatum (SY177), Pulicaria crispa (SY179), and Achillea beiberstenii (SY-200) on cell cycle distribution, apoptosis, caspases activities and mitochondrial function in leukemia CCRF-CEM cell line. METHODS: A resazurin assay was used to assess the cytotoxicity of the extracts on a panel of human cancer cell lines whilst the microbroth dilution was used to determine the minimal inhibitory concentration (MIC) of the samples against twelve bacterial strains belonging to four species, Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae and Pseudomonas aeruginosa. RESULTS: The best activity on leukemia cell lines were recorded with SY177 (IC50 of 9.94 μg/mL) and SY179 (IC50 of 1.81 μg/mL) against CCRF-CEM as well as Ach-b (IC50 of 9.30 μg/mL) and SY160 (IC50 of 5.06 μg/mL) against HL60 cells. The extracts from SY177 and SY179 were also toxic against the seven solid cancer cell lines studied with the highest IC50 values of 31.64 μg/mL (SY177 against Hep-G2 cells). SY177 and Ach-b induced cell cycle arrest in G0/G1 and S phases whilst SY160 and SY179 induced arrest in G0/G1 phase. All the four plant extracts induced apoptosis in CCRF-CEM cells with the alteration of the mitochondrial membrane potential. In the antibacterial assays, only Ach-b displayed moderate antibacterial activities against E. coli and E. aerogenes ATCC strains (MIC of 256 μg/mL), AG100A(TeT) and K. pneumoniae ATCC strains (MIC of 128 μg/mL). CONCLUSIONS: Finally, the results of the present investigation provided supportive data for the possible use of some Saudi Arabian plants investigated herein, and mostly Haplophyllum tuberculatum, Pulicaria crispa, Ononis serrata and Achillea beiberstenii in the control of cancer diseases.
- Date of acceptance
- 2013
- Autoren
- Victor Kuete
- Benjamin Wiench
- Mansour S Alsaid
- Muhammad A Alyahya
- Aimé G Fankam
- Abdelaaty A Shahat
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/24330397
- DOI
- 10.1186/1472-6882-13-354
- eISSN
- 1472-6882
- Externe Identifier
- PubMed Central ID: PMC4029389
- Zeitschrift
- BMC Complement Altern Med
- Schlüsselwörter
- Anti-Bacterial Agents
- Antineoplastic Agents
- Apoptosis
- Caspases
- Cell Cycle
- Cell Line, Tumor
- Cell Survival
- Enterobacteriaceae
- Humans
- Membrane Potential, Mitochondrial
- Microbial Sensitivity Tests
- Plant Extracts
- Plants, Medicinal
- Pseudomonas
- Sprache
- eng
- Country
- England
- Paginierung
- 354
- PII
- 1472-6882-13-354
- Datum der Veröffentlichung
- 2013
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2014
- Titel
- Cytotoxicity, mode of action and antibacterial activities of selected Saudi Arabian medicinal plants.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 13
Datenquelle: PubMed
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