Identification of NF-κB as Determinant of Posttraumatic Stress Disorder and Its Inhibition by the Chinese Herbal Remedy Free and Easy Wanderer
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Chunlan Hong
- Anja Schueffler
- Ulrich Kauhl
- Jingming Cao
- Ching-Fen Wu
- Till Opatz
- Eckhard Thines
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000398654700001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3389/fphar.2017.00181
- eISSN
- 1663-9812
- Externe Identifier
- Clarivate Analytics Document Solution ID: ER2WO
- PubMed Identifier: 28428751
- Zeitschrift
- FRONTIERS IN PHARMACOLOGY
- Schlüsselwörter
- free and easy wanderer
- inflammation
- NF-kappa B
- pharmacognosy
- posttraumatic stress disorder
- Artikelnummer
- ARTN 181
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Titel
- Identification of NF-κB as Determinant of Posttraumatic Stress Disorder and Its Inhibition by the Chinese Herbal Remedy <i>Free</i> and <i>Easy Wanderer</i>
- Sub types
- Article
- Ausgabe der Zeitschrift
- 8
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Chunlan Hong
- Anja Schüffler
- Ulrich Kauhl
- Jingming Cao
- Ching-Fen Wu
- Till Opatz
- Eckhard Thines
- Thomas Efferth
- DOI
- 10.3389/fphar.2017.00181
- eISSN
- 1663-9812
- Zeitschrift
- Frontiers in Pharmacology
- Online publication date
- 2017
- Status
- Published online
- Herausgeber
- Frontiers Media SA
- Herausgeber URL
- http://dx.doi.org/10.3389/fphar.2017.00181
- Datum der Datenerfassung
- 2017
- Titel
- Identification of NF-κB as Determinant of Posttraumatic Stress Disorder and Its Inhibition by the Chinese Herbal Remedy Free and Easy Wanderer
- Ausgabe der Zeitschrift
- 8
Datenquelle: Crossref
- Abstract
- Posttraumatic stress disorder (PTSD) is a mental disorder developing after exposure to traumatic events. Although psychotherapy reveals some therapeutic effectiveness, clinically sustainable cure is still uncertain. Some Chinese herbal formulae are reported to work well clinically against mental diseases in Asian countries, but the safety and their mode of action are still unclear. In this study, we investigated the mechanisms of Chinese remedy <i>free and easy wanderer</i> (FAEW) on PTSD. We used a reverse pharmacology approach combining clinical data to search for mechanisms of PTSD with subsequent <i>in vitro</i> verification and bioinformatics techniques as follows: (1) by analyzing microarray-based transcriptome-wide mRNA expression profiling of PTSD patients; (2) by investigating the effect of FAEW and the antidepressant control drug fluoxetine on the transcription factor NF-κB using reporter cell assays and western blotting; (3) by performing molecular docking and literature data mining based on phytochemical constituents of FAEW. The results suggest an involvement of inflammatory processes mediated through NF-κB in the progression of PTSD. FAEW was non-cytotoxic <i>in vitro</i> and inhibited NF-κB activity and p65 protein expression. FAEW's anti-inflammatory compounds, i.e., paeoniflorin, isoliquiritin, isoliquiritin apioside and ononin were evaluated for binding to IκK and p65-RelA in a molecular docking approach. Paeoniflorin, albiflorin, baicalin, isoliquiritin and liquiritin have been reported to relieve depression <i>in vivo</i> or in clinical trials, which might be the active ingredients for FAEW against PTSD.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg UniversityMainz, Germany.
- Autoren
- Chunlan Hong
- Anja Schüffler
- Ulrich Kauhl
- Jingming Cao
- Ching-Fen Wu
- Till Opatz
- Eckhard Thines
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.3389/fphar.2017.00181
- eISSN
- 1663-9812
- Externe Identifier
- PubMed Identifier: 28428751
- PubMed Central ID: PMC5382210
- Open access
- true
- ISSN
- 1663-9812
- Zeitschrift
- Frontiers in pharmacology
- Sprache
- eng
- Medium
- Electronic-eCollection
- Online publication date
- 2017
- Open access status
- Open Access
- Paginierung
- 181
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2017
- Titel
- Identification of NF-κB as Determinant of Posttraumatic Stress Disorder and Its Inhibition by the Chinese Herbal Remedy <i>Free and Easy Wanderer</i>.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 8
Files
https://www.frontiersin.org/articles/10.3389/fphar.2017.00181/pdf https://europepmc.org/articles/PMC5382210?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Posttraumatic stress disorder (PTSD) is a mental disorder developing after exposure to traumatic events. Although psychotherapy reveals some therapeutic effectiveness, clinically sustainable cure is still uncertain. Some Chinese herbal formulae are reported to work well clinically against mental diseases in Asian countries, but the safety and their mode of action are still unclear. In this study, we investigated the mechanisms of Chinese remedy free and easy wanderer (FAEW) on PTSD. We used a reverse pharmacology approach combining clinical data to search for mechanisms of PTSD with subsequent in vitro verification and bioinformatics techniques as follows: (1) by analyzing microarray-based transcriptome-wide mRNA expression profiling of PTSD patients; (2) by investigating the effect of FAEW and the antidepressant control drug fluoxetine on the transcription factor NF-κB using reporter cell assays and western blotting; (3) by performing molecular docking and literature data mining based on phytochemical constituents of FAEW. The results suggest an involvement of inflammatory processes mediated through NF-κB in the progression of PTSD. FAEW was non-cytotoxic in vitro and inhibited NF-κB activity and p65 protein expression. FAEW's anti-inflammatory compounds, i.e., paeoniflorin, isoliquiritin, isoliquiritin apioside and ononin were evaluated for binding to IκK and p65-RelA in a molecular docking approach. Paeoniflorin, albiflorin, baicalin, isoliquiritin and liquiritin have been reported to relieve depression in vivo or in clinical trials, which might be the active ingredients for FAEW against PTSD.
- Date of acceptance
- 2017
- Autoren
- Chunlan Hong
- Anja Schüffler
- Ulrich Kauhl
- Jingming Cao
- Ching-Fen Wu
- Till Opatz
- Eckhard Thines
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/28428751
- DOI
- 10.3389/fphar.2017.00181
- Externe Identifier
- PubMed Central ID: PMC5382210
- ISSN
- 1663-9812
- Zeitschrift
- Front Pharmacol
- Schlüsselwörter
- NF-κB
- free and easy wanderer
- inflammation
- pharmacognosy
- posttraumatic stress disorder
- Sprache
- eng
- Country
- Switzerland
- Paginierung
- 181
- Datum der Veröffentlichung
- 2017
- Status
- Published online
- Titel
- Identification of NF-κB as Determinant of Posttraumatic Stress Disorder and Its Inhibition by the Chinese Herbal Remedy Free and Easy Wanderer.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 8
Datenquelle: PubMed
- Beziehungen:
- Eigentum von