Acute effects of strength exercises and effects of regular strength training on cell free DNA concentrations in blood plasma

Autoren

Suzan Tug
Anna-Katharina Tross
Patrick Hegen
Elmo Wanja Immanuel Neuberger
Susanne Helmig
Wolfgang Schoellhorn
Perikles Simon

Autoren-URL

https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000410859200075&DestLinkType=FullRecord&DestApp=WOS_CPL

DOI

10.1371/journal.pone.0184668

Externe Identifier

Clarivate Analytics Document Solution ID: FH0VR
PubMed Identifier: 28910365

ISSN

1932-6203

Ausgabe der Veröffentlichung

9

Zeitschrift

PLOS ONE

Artikelnummer

ARTN e0184668

Datum der Veröffentlichung

2017

Status

Published

Titel

Acute effects of strength exercises and effects of regular strength training on cell free DNA concentrations in blood plasma

Sub types

Article

Ausgabe der Zeitschrift

12

Datenquelle: Web of Science (Lite)

Autoren

Suzan Tug
Anna-Katharina Tross
Patrick Hegen
Elmo Wanja Immanuel Neuberger
Susanne Helmig
Wolfgang Schöllhorn
Perikles Simon

DOI

10.1371/journal.pone.0184668

Editoren

Stephen E Alway

eISSN

1932-6203

Ausgabe der Veröffentlichung

9

Zeitschrift

PLOS ONE

Sprache

en

Online publication date

2017

Paginierung

e0184668 - e0184668

Status

Published online

Herausgeber

Public Library of Science (PLoS)

Herausgeber URL

http://dx.doi.org/10.1371/journal.pone.0184668

Datum der Datenerfassung

2018

Titel

Acute effects of strength exercises and effects of regular strength training on cell free DNA concentrations in blood plasma

Ausgabe der Zeitschrift

12

Datenquelle: Crossref

Abstract

Creatine kinase (CK) is a marker for muscle cell damage with limited potential as marker for training load in strength training. Recent exercise studies identified cell free DNA (cfDNA) as a marker for aseptic inflammation and cell damage. Here we overserved in a pilot study the acute effects during strength exercise and chronic effects of regular strength training on cfDNA concentrations over a period of four weeks in three training groups applying conservation training (CT) at 60% of the 1 repetition maximum, high intensity-low repetition training (HT) at 90% of the 1 repetition maximum and differential training (DT) at 60% of the 1 repetition maximum. EDTA-plasma samples were collected before every training session, and on the first and last training day repeatedly after every set of exercises. CfDNA increased significantly by 1.62-fold (mean (±SD) before first exercise: 8.31 (2.84) ng/ml, after last exercise 13.48 (4.12) ng/ml) across all groups within a single training session (p<0.001). The increase was 1.77-fold higher (mean (±SD) before first exercise: 12.23 (6.29) ng/ml, after last exercise 17.73 (11.24) ng/ml) in HT compared to CT (mean (±SD) before first exercise: 6.79 (1.28) ng/ml, after last exercise 10.05 (2.89) ng/ml) (p = 0.01). DNA size analysis suggested predominant release of short, mononucleosomal DNA-fragments in the acute exercise setting, while we detected an increase of mostly longer, polynucleosomal cfDNA-fragments at rest before the training session only at day two with a subsequent return to baseline (p<0.001). In contrast, training procedures did not cause any alterations in CK. Our results suggest that during strength exercise short-fragmented cfDNA is released, reflecting a fast, aseptic inflammatory response, while elevation of longer fragments at baseline on day two seemed to reflect mild cellular damage due to a novel training regime. We critically discuss the implications of our findings for future evaluations of cfDNA as a marker for training load in strength training.

Addresses

Department of Sports Medicine, Disease Prevention and Rehabilitation, Johannes Gutenberg-University Mainz, Mainz, Germany.

Autoren

Suzan Tug
Anna-Katharina Tross
Patrick Hegen
Elmo Wanja Immanuel Neuberger
Susanne Helmig
Wolfgang Schöllhorn
Perikles Simon

DOI

10.1371/journal.pone.0184668

eISSN

1932-6203

Externe Identifier

PubMed Identifier: 28910365
PubMed Central ID: PMC5599009

Open access

true

ISSN

1932-6203

Ausgabe der Veröffentlichung

9

Zeitschrift

PloS one

Schlüsselwörter

Cell-Free System
Humans
DNA Damage
Creatine Kinase
DNA
Exercise
Pilot Projects
Adaptation, Physiological
Physical Endurance
Adult
Male
Resistance Training
Young Adult

Sprache

eng

Medium

Electronic-eCollection

Online publication date

2017

Open access status

Open Access

Paginierung

e0184668

Datum der Veröffentlichung

2017

Status

Published

Publisher licence

CC BY

Datum der Datenerfassung

2017

Titel

Acute effects of strength exercises and effects of regular strength training on cell free DNA concentrations in blood plasma.

Sub types

research-article
Journal Article

Ausgabe der Zeitschrift

12

Files

https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0184668&type=printable https://europepmc.org/articles/PMC5599009?pdf=render

Datenquelle: Europe PubMed Central

Abstract

Creatine kinase (CK) is a marker for muscle cell damage with limited potential as marker for training load in strength training. Recent exercise studies identified cell free DNA (cfDNA) as a marker for aseptic inflammation and cell damage. Here we overserved in a pilot study the acute effects during strength exercise and chronic effects of regular strength training on cfDNA concentrations over a period of four weeks in three training groups applying conservation training (CT) at 60% of the 1 repetition maximum, high intensity-low repetition training (HT) at 90% of the 1 repetition maximum and differential training (DT) at 60% of the 1 repetition maximum. EDTA-plasma samples were collected before every training session, and on the first and last training day repeatedly after every set of exercises. CfDNA increased significantly by 1.62-fold (mean (±SD) before first exercise: 8.31 (2.84) ng/ml, after last exercise 13.48 (4.12) ng/ml) across all groups within a single training session (p<0.001). The increase was 1.77-fold higher (mean (±SD) before first exercise: 12.23 (6.29) ng/ml, after last exercise 17.73 (11.24) ng/ml) in HT compared to CT (mean (±SD) before first exercise: 6.79 (1.28) ng/ml, after last exercise 10.05 (2.89) ng/ml) (p = 0.01). DNA size analysis suggested predominant release of short, mononucleosomal DNA-fragments in the acute exercise setting, while we detected an increase of mostly longer, polynucleosomal cfDNA-fragments at rest before the training session only at day two with a subsequent return to baseline (p<0.001). In contrast, training procedures did not cause any alterations in CK. Our results suggest that during strength exercise short-fragmented cfDNA is released, reflecting a fast, aseptic inflammatory response, while elevation of longer fragments at baseline on day two seemed to reflect mild cellular damage due to a novel training regime. We critically discuss the implications of our findings for future evaluations of cfDNA as a marker for training load in strength training.

Date of acceptance

2017

Autoren

Suzan Tug
Anna-Katharina Tross
Patrick Hegen
Elmo Wanja Immanuel Neuberger
Susanne Helmig
Wolfgang Schöllhorn
Perikles Simon

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/28910365

DOI

10.1371/journal.pone.0184668

eISSN

1932-6203

Externe Identifier

PubMed Central ID: PMC5599009

Ausgabe der Veröffentlichung

9

Zeitschrift

PLoS One

Schlüsselwörter

Adaptation, Physiological
Adult
Cell-Free System
Creatine Kinase
DNA
DNA Damage
Exercise
Humans
Male
Physical Endurance
Pilot Projects
Resistance Training
Young Adult

Sprache

eng

Country

United States

Paginierung

e0184668

PII

PONE-D-17-11237

Datum der Veröffentlichung

2017

Status

Published online

Datum, an dem der Datensatz öffentlich gemacht wurde

2017

Titel

Acute effects of strength exercises and effects of regular strength training on cell free DNA concentrations in blood plasma.

Sub types

Journal Article

Ausgabe der Zeitschrift

12

Datenquelle: PubMed

Author's licence

CC-BY

Autoren

Suzan Tug
Anna-Katharina Tross
Patrick Hegen
Elmo Neuberger
Susanne Helmig
Wolfgang Schöllhorn
Perikles Simon

Hosting institution

Universitätsbibliothek Mainz

Sammlungen

DFG-OA-Publizieren (2012 - 2017)

Resource version

Published version

DOI

10.1371/journal.pone.0184668

Funding acknowledgements

DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin

File(s) embargoed

false

Open access

true

ISSN

1932-6203

Ausgabe der Veröffentlichung

9

Zeitschrift

PLoS one

Schlüsselwörter

796 Sport
796 Athletic and outdoor sports and games

Sprache

eng

Open access status

Open Access

Paginierung

e0184668

Datum der Veröffentlichung

2017

Public URL

https://openscience.ub.uni-mainz.de/handle/20.500.12030/7397

Herausgeber

PLoS

Herausgeber URL

http://dx.doi.org/10.1371/journal.pone.0184668

Datum der Datenerfassung

2022

Datum, an dem der Datensatz öffentlich gemacht wurde

2022

Zugang

Public

Titel

Acute effects of strength exercises and effects of regular strength training on cell free DNA concentrations in blood plasma

Ausgabe der Zeitschrift

12

Files

acute_effects_of_strength_exe-20220710235013206.pdf

Datenquelle: OPENSCIENCE.UB

Acute effects of strength exercises and effects of regular strength training on cell free DNA concentrations in blood plasma

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