Short-term treadmill running as a model for studying cell-free DNA kinetics in vivo

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Thomas Beiter
  • Annunziata Fragasso
  • Jens Hudemann
  • Andreas M Nieß
  • Perikles Simon
Sammlungen
  • metadata
ISSN
1530-8561
Ausgabe der Veröffentlichung
4
Zeitschrift
Clinical chemistry
Schlüsselwörter
  • 610 Medizin
  • 610 Medical sciences
Sprache
eng
Paginierung
Seiten: 633 - 636
Datum der Veröffentlichung
2011
Herausgeber URL
http://dx.doi.org/10.1373/clinchem.2010.158030
Datum der Datenerfassung
2020
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Zugang
Public
Titel
Short-term treadmill running as a model for studying cell-free DNA kinetics in vivo
Ausgabe der Zeitschrift
57

Datenquelle: METADATA.UB

Andere Metadatenquellen:
Autoren
  • Thomas Beiter
  • Annunziata Fragasso
  • Jens Hudemann
  • Andreas M Niess
  • Perikles Simon
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000288854000018&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1373/clinchem.2010.158030
Externe Identifier
  • Clarivate Analytics Document Solution ID: 741HE
  • PubMed Identifier: 21296972
ISSN
0009-9147
Ausgabe der Veröffentlichung
4
Zeitschrift
CLINICAL CHEMISTRY
Paginierung
633 - 636
Datum der Veröffentlichung
2011
Status
Published
Titel
Short-Term Treadmill Running as a Model for Studying Cell-Free DNA Kinetics In Vivo
Sub types
  • Article
Ausgabe der Zeitschrift
57

Datenquelle: Web of Science (Lite)

Abstract
<jats:sec> <jats:title>BACKGROUND</jats:title> <jats:p>Increased plasma concentrations of cell-free DNA (cf-DNA) are considered a hallmark of various clinical conditions. Despite intensive research in this field, limited data are available concerning the time course of release and clearance of cf-DNA in vivo.</jats:p> </jats:sec> <jats:sec> <jats:title>METHODS</jats:title> <jats:p>We extracted cf-DNA from plasma samples taken before and immediately after a 10-km cross-country run, and from samples taken before, immediately after, and 30 min after exhaustive short-term treadmill exercise. The contribution of nuclear (nDNA) and mitochondrial DNA (mtDNA) was measured by quantitative real-time PCR. The incremental treadmill exercise setup was exploited to delineate the precise sequencing and timing of cf-nDNA, lactate, and high-mobility group box 1 protein (HMGB1) release during the exercise and recovery phases.</jats:p> </jats:sec> <jats:sec> <jats:title>RESULTS</jats:title> <jats:p>Postexercise plasma cf-nDNA concentrations in cross-country and treadmill runners were significantly increased, by 7.6-fold and 9.9-fold, respectively (P &amp;lt; 0.001). cf-nDNA concentrations were not correlated with age, sex, or body mass index. Plasma concentrations of cf-nDNA and HMGB1 in postexercise samples of treadmill runners were significantly correlated (r = 0.84; P = 0.004). cf-mtDNA concentrations were not affected by treadmill exercise. Time-course analyses demonstrated that cf-nDNA is released within minutes after the onset of exercise and is rapidly cleared from the circulation after the cessation of exercise. Nearly congruent kinetics for cf-nDNA, lactate, and HMGB1 were observed during the exercise phase.</jats:p> </jats:sec> <jats:sec> <jats:title>CONCLUSIONS</jats:title> <jats:p>A single bout of exhaustive short-term treadmill exercise constitutes a versatile model system suitable for addressing basic questions about cf-DNA biology.</jats:p> </jats:sec>
Autoren
  • Thomas Beiter
  • Annunziata Fragasso
  • Jens Hudemann
  • Andreas M Nieß
  • Perikles Simon
DOI
10.1373/clinchem.2010.158030
eISSN
1530-8561
ISSN
0009-9147
Ausgabe der Veröffentlichung
4
Zeitschrift
Clinical Chemistry
Sprache
en
Online publication date
2011
Paginierung
633 - 636
Datum der Veröffentlichung
2011
Status
Published
Herausgeber
Oxford University Press (OUP)
Herausgeber URL
http://dx.doi.org/10.1373/clinchem.2010.158030
Datum der Datenerfassung
2020
Titel
Short-Term Treadmill Running as a Model for Studying Cell-Free DNA Kinetics In Vivo
Ausgabe der Zeitschrift
57

Datenquelle: Crossref

Abstract
<h4>Background</h4>Increased plasma concentrations of cell-free DNA (cf-DNA) are considered a hallmark of various clinical conditions. Despite intensive research in this field, limited data are available concerning the time course of release and clearance of cf-DNA in vivo.<h4>Methods</h4>We extracted cf-DNA from plasma samples taken before and immediately after a 10-km cross-country run, and from samples taken before, immediately after, and 30 min after exhaustive short-term treadmill exercise. The contribution of nuclear (nDNA) and mitochondrial DNA (mtDNA) was measured by quantitative real-time PCR. The incremental treadmill exercise setup was exploited to delineate the precise sequencing and timing of cf-nDNA, lactate, and high-mobility group box 1 protein (HMGB1) release during the exercise and recovery phases.<h4>Results</h4>Postexercise plasma cf-nDNA concentrations in cross-country and treadmill runners were significantly increased, by 7.6-fold and 9.9-fold, respectively (P < 0.001). cf-nDNA concentrations were not correlated with age, sex, or body mass index. Plasma concentrations of cf-nDNA and HMGB1 in postexercise samples of treadmill runners were significantly correlated (r = 0.84; P = 0.004). cf-mtDNA concentrations were not affected by treadmill exercise. Time-course analyses demonstrated that cf-nDNA is released within minutes after the onset of exercise and is rapidly cleared from the circulation after the cessation of exercise. Nearly congruent kinetics for cf-nDNA, lactate, and HMGB1 were observed during the exercise phase.<h4>Conclusions</h4>A single bout of exhaustive short-term treadmill exercise constitutes a versatile model system suitable for addressing basic questions about cf-DNA biology.
Addresses
  • Department of Sports Medicine, Eberhard-Karls-University of Tuebingen, Tuebingen, Germany. thomas.beiter@med.uni-tuebingen.de
Autoren
  • Thomas Beiter
  • Annunziata Fragasso
  • Jens Hudemann
  • Andreas M Niess
  • Perikles Simon
DOI
10.1373/clinchem.2010.158030
eISSN
1530-8561
Externe Identifier
  • PubMed Identifier: 21296972
Funding acknowledgements
  • Bundesinstitut für Sportwissenschaft: IIA1-080308/08
Open access
false
ISSN
0009-9147
Ausgabe der Veröffentlichung
4
Zeitschrift
Clinical chemistry
Schlüsselwörter
  • Cell-Free System
  • Humans
  • DNA
  • Running
  • Kinetics
  • Adolescent
  • Adult
  • Middle Aged
  • Female
  • Male
  • Young Adult
Sprache
eng
Medium
Print-Electronic
Online publication date
2011
Paginierung
633 - 636
Datum der Veröffentlichung
2011
Status
Published
Datum der Datenerfassung
2011
Titel
Short-term treadmill running as a model for studying cell-free DNA kinetics in vivo.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
57

Datenquelle: Europe PubMed Central

Abstract
BACKGROUND: Increased plasma concentrations of cell-free DNA (cf-DNA) are considered a hallmark of various clinical conditions. Despite intensive research in this field, limited data are available concerning the time course of release and clearance of cf-DNA in vivo. METHODS: We extracted cf-DNA from plasma samples taken before and immediately after a 10-km cross-country run, and from samples taken before, immediately after, and 30 min after exhaustive short-term treadmill exercise. The contribution of nuclear (nDNA) and mitochondrial DNA (mtDNA) was measured by quantitative real-time PCR. The incremental treadmill exercise setup was exploited to delineate the precise sequencing and timing of cf-nDNA, lactate, and high-mobility group box 1 protein (HMGB1) release during the exercise and recovery phases. RESULTS: Postexercise plasma cf-nDNA concentrations in cross-country and treadmill runners were significantly increased, by 7.6-fold and 9.9-fold, respectively (P < 0.001). cf-nDNA concentrations were not correlated with age, sex, or body mass index. Plasma concentrations of cf-nDNA and HMGB1 in postexercise samples of treadmill runners were significantly correlated (r = 0.84; P = 0.004). cf-mtDNA concentrations were not affected by treadmill exercise. Time-course analyses demonstrated that cf-nDNA is released within minutes after the onset of exercise and is rapidly cleared from the circulation after the cessation of exercise. Nearly congruent kinetics for cf-nDNA, lactate, and HMGB1 were observed during the exercise phase. CONCLUSIONS: A single bout of exhaustive short-term treadmill exercise constitutes a versatile model system suitable for addressing basic questions about cf-DNA biology.
Autoren
  • Thomas Beiter
  • Annunziata Fragasso
  • Jens Hudemann
  • Andreas M Niess
  • Perikles Simon
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/21296972
DOI
10.1373/clinchem.2010.158030
eISSN
1530-8561
Ausgabe der Veröffentlichung
4
Zeitschrift
Clin Chem
Schlüsselwörter
  • Adolescent
  • Adult
  • Cell-Free System
  • DNA
  • Female
  • Humans
  • Kinetics
  • Male
  • Middle Aged
  • Running
  • Young Adult
Sprache
eng
Country
England
Paginierung
633 - 636
PII
clinchem.2010.158030
Datum der Veröffentlichung
2011
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2011
Titel
Short-term treadmill running as a model for studying cell-free DNA kinetics in vivo.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
57

Datenquelle: PubMed

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