Characterization of the epithelial permeation enhancing effect of basic Butylated methacrylate copolymer - in vitro studies

Autoren

Stefan Grube
Uwe Wolfrurn
Peter Langguth

Autoren-URL

https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000255766700007&DestLinkType=FullRecord&DestApp=WOS_CPL

DOI

10.1021/bm701337q

eISSN

1526-4602

Externe Identifier

Clarivate Analytics Document Solution ID: 299PE
PubMed Identifier: 18407686

ISSN

1525-7797

Ausgabe der Veröffentlichung

5

Zeitschrift

BIOMACROMOLECULES

Paginierung

1398 - 1405

Datum der Veröffentlichung

2008

Status

Published

Titel

Characterization of the epithelial permeation enhancing effect of basic butylated methacrylate copolymer - In vitro studies

Sub types

Article

Ausgabe der Zeitschrift

9

Datenquelle: Web of Science (Lite)

Autoren

Stefan Grube
Uwe Wolfrum
Peter Langguth

DOI

10.1021/bm701337q

eISSN

1526-4602

ISSN

1525-7797

Ausgabe der Veröffentlichung

5

Zeitschrift

Biomacromolecules

Sprache

en

Online publication date

2008

Paginierung

1398 - 1405

Datum der Veröffentlichung

2008

Status

Published

Herausgeber

American Chemical Society (ACS)

Herausgeber URL

http://dx.doi.org/10.1021/bm701337q

Datum der Datenerfassung

2023

Titel

Characterization of the Epithelial Permeation Enhancing Effect of Basic Butylated Methacrylate Copolymer—In Vitro Studies

Ausgabe der Zeitschrift

9

Datenquelle: Crossref

Abstract

Membrane destabilizing properties and increased efflux of doxorubicin from liposomes caused by basic butylated methacrylate copolymer (BBMC), better known under its commercial trade name EUDRAGIT E, have been described in the scientific literature. Here, we investigated the effect of BBMC on suspended and filter-grown Caco2 cells with respect to apical-to-basal transport and membrane permeabilization using transport assays, trypan blue exclusion assay, measurements of transepithelial electrical resistance (TEER), confocal laser scanning microscopy, and transmission electron microscopy. The effect of inhibiting protein phosphatase 2A (PP2A) by okadaic acid was investigated by measuring TEER, but a link between PP2A and the observed effects could not be established. Overall, membrane permeabilization of Caco2 cells by BBMC was demonstrated, which went along with increased apical-to-basal transport of the model compounds mannitol, talinolol, and trospium. The effect was concentration- and time-dependent, and reversible. Enhancement occurred at polymer concentrations as low as 20 microg/mL.

Addresses

Department of Biopharmaceutics and Pharmaceutical Technology, Johannes Gutenberg University, Saudinger Weg 5, Mainz, Germany.

Autoren

Stefan Grube
Uwe Wolfrum
Peter Langguth

DOI

10.1021/bm701337q

eISSN

1526-4602

Externe Identifier

PubMed Identifier: 18407686

Open access

false

ISSN

1525-7797

Ausgabe der Veröffentlichung

5

Zeitschrift

Biomacromolecules

Schlüsselwörter

Caco-2 Cells
Epithelial Cells
Humans
Methacrylates
Methylmethacrylates
Cell Membrane Permeability
Cell Polarity
Biological Transport
Dose-Response Relationship, Drug
Kinetics
Protein Phosphatase 2

Sprache

eng

Medium

Print-Electronic

Online publication date

2008

Paginierung

1398 - 1405

Datum der Veröffentlichung

2008

Status

Published

Datum der Datenerfassung

2008

Titel

Characterization of the epithelial permeation enhancing effect of basic butylated methacrylate copolymer--in vitro studies.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

9

Datenquelle: Europe PubMed Central

Abstract

Membrane destabilizing properties and increased efflux of doxorubicin from liposomes caused by basic butylated methacrylate copolymer (BBMC), better known under its commercial trade name EUDRAGIT E, have been described in the scientific literature. Here, we investigated the effect of BBMC on suspended and filter-grown Caco2 cells with respect to apical-to-basal transport and membrane permeabilization using transport assays, trypan blue exclusion assay, measurements of transepithelial electrical resistance (TEER), confocal laser scanning microscopy, and transmission electron microscopy. The effect of inhibiting protein phosphatase 2A (PP2A) by okadaic acid was investigated by measuring TEER, but a link between PP2A and the observed effects could not be established. Overall, membrane permeabilization of Caco2 cells by BBMC was demonstrated, which went along with increased apical-to-basal transport of the model compounds mannitol, talinolol, and trospium. The effect was concentration- and time-dependent, and reversible. Enhancement occurred at polymer concentrations as low as 20 microg/mL.

Autoren

Stefan Grube
Uwe Wolfrum
Peter Langguth

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/18407686

DOI

10.1021/bm701337q

eISSN

1526-4602

Ausgabe der Veröffentlichung

5

Zeitschrift

Biomacromolecules

Schlüsselwörter

Biological Transport
Caco-2 Cells
Cell Membrane Permeability
Cell Polarity
Dose-Response Relationship, Drug
Epithelial Cells
Humans
Kinetics
Methacrylates
Methylmethacrylates
Protein Phosphatase 2

Sprache

eng

Country

United States

Paginierung

1398 - 1405

Datum der Veröffentlichung

2008

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2008

Titel

Characterization of the epithelial permeation enhancing effect of basic butylated methacrylate copolymer--in vitro studies.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

9

Datenquelle: PubMed

Characterization of the epithelial permeation enhancing effect of basic Butylated methacrylate copolymer - in vitro studies

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