Exercise-induced increases in cell free DNA in human plasma originate predominantly from cells of the haematopoietic lineage

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Suzan Tug
  • Susanne Helmig
  • Eva Ricarda Deichmann
  • Anna Schmeier-Jürchott
  • Eva Wagner
  • Tim Zimmermann
  • Markus Radsak
  • Mauro Giacca
  • Perikles Simon
Sammlungen
  • metadata
ISSN
1077-5552
Zeitschrift
Exercise immunology review
Schlüsselwörter
  • 796 Sport
  • 796 Athletic and outdoor sports and games
Sprache
eng
Paginierung
Seiten: 164 - 173
Datum der Veröffentlichung
2015
Herausgeber
ISEI
Herausgeber URL
http://www.medizin.uni-tuebingen.de/transfusionsmedizin/institut/eir/content/2015/164/article.pdf
Datum der Datenerfassung
2020
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Zugang
Public
Titel
Exercise-induced increases in cell free DNA in human plasma originate predominantly from cells of the haematopoietic lineage
Ausgabe der Zeitschrift
21

Datenquelle: METADATA.UB

Andere Metadatenquellen:
Autoren
  • Suzan Tug
  • Susanne Helmig
  • Eva Ricarda Deichmann
  • Anna Schmeier-Juerchott
  • Eva Wagner
  • Tim Zimmermann
  • Markus Radsak
  • Mauro Giacca
  • Perikles Simon
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000363221100012&DestLinkType=FullRecord&DestApp=WOS_CPL
Externe Identifier
  • Clarivate Analytics Document Solution ID: CU0QA
  • PubMed Identifier: 25826002
ISSN
1077-5552
Zeitschrift
EXERCISE IMMUNOLOGY REVIEW
Schlüsselwörter
  • cell free DNA
  • sex-mismatched transplantation
  • Y-chromosomal PCR
  • exercise
  • graft rejection
Paginierung
164 - 173
Datum der Veröffentlichung
2015
Status
Published
Titel
Exercise-induced increases in cell free DNA in human plasma originate predominantly from cells of the haematopoietic lineage
Sub types
  • Review
Ausgabe der Zeitschrift
21

Datenquelle: Web of Science (Lite)

Abstract
The role of cell free DNA (cfDNA) has been intensively discussed under various pathological conditions and after acute bouts of exercise. To date, there is still no conclusive evidence concerning the cellular origin of cfDNA and the entire mechanism leading to elevated cfDNA concentrations in human plasma and serum. Here, we investigated the cellular origin of cfDNA in sex-mismatched haematopoietic stem cell transplantation (HSCT) and liver transplantation (LT) patients by determining the relative proportion of Y-chromosomal to total nuclear cfDNA. Total nuclear cfDNA and Y-chromosomal cfDNA concentrations were determined in blood plasma before and after an incremental exercise test via quantitative real-time PCR (qPCR). Female HSCT patients showed high proportions of Y-chromosomal cfDNA. Both total nuclear and Y-chromosomal cfDNA increased significantly and in a highly correlated fashion due to exercise. In male HSCT patients with female donors less than 10% of the cfDNA was of Y-chromosomal origin at any point in time and even though the total amount of cfDNA increased during exercise, no increases in Y-chromosomal DNA could be detected. The percentage of Y-chromosomal cfDNA in female LT patients with male donors was very low and levels remained unchanged during exercise. This indicates that cells not derived from the bone marrow, in this case transplanted liver cells, represented only a minor fraction of cfDNA in blood plasma and were not released during acute physical exercise. Even though many physiological conditions may be altered in transplant patients versus healthy people, our results strongly suggest that cells from the haematopoietic lineage are the main source of cfDNA released during acute bouts of exercise.
Addresses
  • Department of Sports Medicine, Disease Prevention and Rehabilitation, Johannes Gutenberg-University Mainz, Mainz, Germany.
Autoren
  • Suzan Tug
  • Susanne Helmig
  • Eva Ricarda Deichmann
  • Anna Schmeier-Jürchott
  • Eva Wagner
  • Tim Zimmermann
  • Markus Radsak
  • Mauro Giacca
  • Perikles Simon
eISSN
2959-9555
Externe Identifier
  • PubMed Identifier: 25826002
Open access
false
ISSN
1077-5552
Zeitschrift
Exercise immunology review
Schlüsselwörter
  • Hematopoietic Stem Cells
  • Chromosomes, Human, Y
  • Cell Nucleus
  • Hepatocytes
  • Plasma
  • Humans
  • DNA
  • Exercise Test
  • Exercise
  • Liver Transplantation
  • Hematopoietic Stem Cell Transplantation
  • Pilot Projects
  • Organ Specificity
  • Cell Lineage
  • Running
  • Adult
  • Middle Aged
  • Tissue Donors
  • Female
  • Male
  • Young Adult
Sprache
eng
Medium
Print
Paginierung
164 - 173
Datum der Veröffentlichung
2015
Status
Published
Datum der Datenerfassung
2015
Titel
Exercise-induced increases in cell free DNA in human plasma originate predominantly from cells of the haematopoietic lineage.
Sub types
  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
21

Datenquelle: Europe PubMed Central

Abstract
The role of cell free DNA (cfDNA) has been intensively discussed under various pathological conditions and after acute bouts of exercise. To date, there is still no conclusive evidence concerning the cellular origin of cfDNA and the entire mechanism leading to elevated cfDNA concentrations in human plasma and serum. Here, we investigated the cellular origin of cfDNA in sex-mismatched haematopoietic stem cell transplantation (HSCT) and liver transplantation (LT) patients by determining the relative proportion of Y-chromosomal to total nuclear cfDNA. Total nuclear cfDNA and Y-chromosomal cfDNA concentrations were determined in blood plasma before and after an incremental exercise test via quantitative real-time PCR (qPCR). Female HSCT patients showed high proportions of Y-chromosomal cfDNA. Both total nuclear and Y-chromosomal cfDNA increased significantly and in a highly correlated fashion due to exercise. In male HSCT patients with female donors less than 10% of the cfDNA was of Y-chromosomal origin at any point in time and even though the total amount of cfDNA increased during exercise, no increases in Y-chromosomal DNA could be detected. The percentage of Y-chromosomal cfDNA in female LT patients with male donors was very low and levels remained unchanged during exercise. This indicates that cells not derived from the bone marrow, in this case transplanted liver cells, represented only a minor fraction of cfDNA in blood plasma and were not released during acute physical exercise. Even though many physiological conditions may be altered in transplant patients versus healthy people, our results strongly suggest that cells from the haematopoietic lineage are the main source of cfDNA released during acute bouts of exercise.
Autoren
  • Suzan Tug
  • Susanne Helmig
  • Eva Ricarda Deichmann
  • Anna Schmeier-Jürchott
  • Eva Wagner
  • Tim Zimmermann
  • Markus Radsak
  • Mauro Giacca
  • Perikles Simon
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/25826002
ISSN
1077-5552
Zeitschrift
Exerc Immunol Rev
Schlüsselwörter
  • Y-chromosomal PCR
  • cell free DNA
  • exercise
  • graft rejection
  • sex-mismatched transplantation
  • Adult
  • Cell Lineage
  • Cell Nucleus
  • Chromosomes, Human, Y
  • DNA
  • Exercise
  • Exercise Test
  • Female
  • Hematopoietic Stem Cell Transplantation
  • Hematopoietic Stem Cells
  • Hepatocytes
  • Humans
  • Liver Transplantation
  • Male
  • Middle Aged
  • Organ Specificity
  • Pilot Projects
  • Plasma
  • Running
  • Tissue Donors
  • Young Adult
Sprache
eng
Country
Germany
Paginierung
164 - 173
Datum der Veröffentlichung
2015
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2015
Titel
Exercise-induced increases in cell free DNA in human plasma originate predominantly from cells of the haematopoietic lineage.
Sub types
  • Comparative Study
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
21

Datenquelle: PubMed

Beziehungen:
Eigentum von

Werkzeuge