The distinct gene regulatory network of myoglobin in prostate and breast cancer

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Anne Bicker
  • Alexandra Brahmer
  • Sebastian Meller
  • Glen Kristiansen
  • Thomas A Gorr
  • Thomas Hankeln
Sammlungen
  • metadata
ISSN
1932-6203
Ausgabe der Veröffentlichung
11
Zeitschrift
PLoS one
Schlüsselwörter
  • 570 Biowissenschaften
  • 570 Life sciences
Sprache
eng
Paginierung
e0142662
Datum der Veröffentlichung
2015
Herausgeber
PLoS
Herausgeber URL
http://dx.doi.org/10.1371/journal.pone.0142662
Datum der Datenerfassung
2020
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Zugang
Public
Titel
The distinct gene regulatory network of myoglobin in prostate and breast cancer
Ausgabe der Zeitschrift
10

Datenquelle: METADATA.UB

Andere Metadatenquellen:
Autoren
  • Anne Bicker
  • Alexandra M Brahmer
  • Sebastian Meller
  • Glen Kristiansen
  • Thomas A Gorr
  • Thomas Hankeln
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000364433100108&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1371/journal.pone.0142662
Externe Identifier
  • Clarivate Analytics Document Solution ID: CV7DR
  • PubMed Identifier: 26559958
ISSN
1932-6203
Ausgabe der Veröffentlichung
11
Zeitschrift
PLOS ONE
Artikelnummer
ARTN e0142662
Datum der Veröffentlichung
2015
Status
Published
Titel
The Distinct Gene Regulatory Network of Myoglobin in Prostate and Breast Cancer
Sub types
  • Article
Ausgabe der Zeitschrift
10

Datenquelle: Web of Science (Lite)

Autoren
  • Anne Bicker
  • Alexandra M Brahmer
  • Sebastian Meller
  • Glen Kristiansen
  • Thomas A Gorr
  • Thomas Hankeln
DOI
10.1371/journal.pone.0142662
Editoren
  • Craig N Robson
eISSN
1932-6203
Ausgabe der Veröffentlichung
11
Zeitschrift
PLOS ONE
Sprache
en
Online publication date
2015
Paginierung
e0142662 - e0142662
Status
Published online
Herausgeber
Public Library of Science (PLoS)
Herausgeber URL
http://dx.doi.org/10.1371/journal.pone.0142662
Datum der Datenerfassung
2022
Titel
The Distinct Gene Regulatory Network of Myoglobin in Prostate and Breast Cancer
Ausgabe der Zeitschrift
10

Datenquelle: Crossref

Abstract
Myoglobin (MB) is not only strongly expressed in myocytes, but also at much lower levels in different cancer entities. 40% of breast tumors are MB-positive, with the globin being co-expressed with markers of tumor hypoxia in a proportion of cases. In breast cancer, MB expression is associated with a positive hormone receptor status and patient prognosis. In prostate cancer, another hormone-dependent cancer type, 53% of tumors were recently shown to express MB. Especially in more aggressive prostate cancer specimen MB expression also correlates with increased patient survival rates. Both findings might be due to tumor-suppressing properties of MB in cancer cells. In contrast to muscle, MB transcription in breast and prostate cancer mainly depends on a novel, alternative promoter site. We show here that its associated transcripts can be upregulated by hypoxia and downregulated by estrogens and androgens in MCF7 breast and LNCaP prostate cancer cells, respectively. Bioinformatic data mining of epigenetic histone marks and experimental verification reveal a hitherto uncharacterized transcriptional network that drives the regulation of the MB gene in cancer cells. We identified candidate hormone-receptor binding elements that may interact with the cancer-associated MB promoter to decrease its activity in breast and prostate cancer cells. Additionally, a regulatory element, 250 kb downstream of the promoter, acts as a hypoxia-inducible site within the transcriptional machinery. Understanding the distinct regulation of MB in tumors will improve unraveling the respiratory protein's function in the cancer context and may provide new starting points for developing therapeutic strategies.
Addresses
  • Institute of Molecular Genetics, Johannes Gutenberg University, Mainz, Germany.
Autoren
  • Anne Bicker
  • Alexandra M Brahmer
  • Sebastian Meller
  • Glen Kristiansen
  • Thomas A Gorr
  • Thomas Hankeln
DOI
10.1371/journal.pone.0142662
eISSN
1932-6203
Externe Identifier
  • PubMed Identifier: 26559958
  • PubMed Central ID: PMC4641586
Open access
true
ISSN
1932-6203
Ausgabe der Veröffentlichung
11
Zeitschrift
PloS one
Schlüsselwörter
  • Cell Line, Tumor
  • Chromatin
  • Humans
  • Breast Neoplasms
  • Prostatic Neoplasms
  • Myoglobin
  • Prognosis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Computational Biology
  • Cell Hypoxia
  • Epigenesis, Genetic
  • Gene Expression Regulation, Neoplastic
  • Binding Sites
  • Genes, Regulator
  • Genes, Reporter
  • Female
  • Male
  • Gene Regulatory Networks
  • Data Mining
Sprache
eng
Medium
Electronic-eCollection
Online publication date
2015
Open access status
Open Access
Paginierung
e0142662
Datum der Veröffentlichung
2015
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2015
Titel
The Distinct Gene Regulatory Network of Myoglobin in Prostate and Breast Cancer.
Sub types
  • Research Support, Non-U.S. Gov't
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
10

Files

https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0142662&type=printable https://europepmc.org/articles/PMC4641586?pdf=render

Datenquelle: Europe PubMed Central

Abstract
Myoglobin (MB) is not only strongly expressed in myocytes, but also at much lower levels in different cancer entities. 40% of breast tumors are MB-positive, with the globin being co-expressed with markers of tumor hypoxia in a proportion of cases. In breast cancer, MB expression is associated with a positive hormone receptor status and patient prognosis. In prostate cancer, another hormone-dependent cancer type, 53% of tumors were recently shown to express MB. Especially in more aggressive prostate cancer specimen MB expression also correlates with increased patient survival rates. Both findings might be due to tumor-suppressing properties of MB in cancer cells. In contrast to muscle, MB transcription in breast and prostate cancer mainly depends on a novel, alternative promoter site. We show here that its associated transcripts can be upregulated by hypoxia and downregulated by estrogens and androgens in MCF7 breast and LNCaP prostate cancer cells, respectively. Bioinformatic data mining of epigenetic histone marks and experimental verification reveal a hitherto uncharacterized transcriptional network that drives the regulation of the MB gene in cancer cells. We identified candidate hormone-receptor binding elements that may interact with the cancer-associated MB promoter to decrease its activity in breast and prostate cancer cells. Additionally, a regulatory element, 250 kb downstream of the promoter, acts as a hypoxia-inducible site within the transcriptional machinery. Understanding the distinct regulation of MB in tumors will improve unraveling the respiratory protein's function in the cancer context and may provide new starting points for developing therapeutic strategies.
Date of acceptance
2015
Autoren
  • Anne Bicker
  • Alexandra M Brahmer
  • Sebastian Meller
  • Glen Kristiansen
  • Thomas A Gorr
  • Thomas Hankeln
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/26559958
DOI
10.1371/journal.pone.0142662
eISSN
1932-6203
Externe Identifier
  • PubMed Central ID: PMC4641586
Ausgabe der Veröffentlichung
11
Zeitschrift
PLoS One
Schlüsselwörter
  • Binding Sites
  • Breast Neoplasms
  • Cell Hypoxia
  • Cell Line, Tumor
  • Chromatin
  • Computational Biology
  • Data Mining
  • Epigenesis, Genetic
  • Female
  • Gene Expression Regulation, Neoplastic
  • Gene Regulatory Networks
  • Genes, Regulator
  • Genes, Reporter
  • Humans
  • Male
  • Myoglobin
  • Prognosis
  • Prostatic Neoplasms
  • Reverse Transcriptase Polymerase Chain Reaction
Sprache
eng
Country
United States
Paginierung
e0142662
PII
PONE-D-15-30342
Datum der Veröffentlichung
2015
Status
Published online
Datum, an dem der Datensatz öffentlich gemacht wurde
2016
Titel
The Distinct Gene Regulatory Network of Myoglobin in Prostate and Breast Cancer.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
10

Datenquelle: PubMed

Author's licence
CC-BY
Autoren
  • Anne Bicker
  • Alexandra Brahmer
  • Sebastian Meller
  • Glen Kristiansen
  • Thomas A Gorr
  • Thomas Hankeln
Hosting institution
Universitätsbibliothek Mainz
Sammlungen
  • DFG-OA-Publizieren (2012 - 2017)
Resource version
Published version
DOI
10.1371/journal.pone.0142662
Funding acknowledgements
  • DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin
File(s) embargoed
false
Open access
true
ISSN
1932-6203
Ausgabe der Veröffentlichung
11
Zeitschrift
PLoS one
Schlüsselwörter
  • 570 Biowissenschaften
  • 570 Life sciences
Sprache
eng
Open access status
Open Access
Paginierung
e0142662
Datum der Veröffentlichung
2015
Public URL
https://openscience.ub.uni-mainz.de/handle/20.500.12030/7405
Herausgeber
PLoS
Herausgeber URL
http://dx.doi.org/10.1371/journal.pone.0142662
Datum der Datenerfassung
2022
Datum, an dem der Datensatz öffentlich gemacht wurde
2022
Zugang
Public
Titel
The distinct gene regulatory network of myoglobin in prostate and breast cancer
Ausgabe der Zeitschrift
10

Files

the_distinct_gene_regulatory_-20220712203015047.pdf

Datenquelle: OPENSCIENCE.UB

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