Compensatory vasodilator mechanisms in the ophthalmic artery of endothelial nitric oxide synthase gene knockout mice
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Caroline Manicam
- Natalja Ginter
- Huige Li
- Ning Xia
- Evgeny Goloborodko
- Jenia Kouchek Zadeh
- Aytan Musayeva
- Norbert Pfeiffer
- Adrian Gericke
- Sammlungen
- metadata
- ISSN
- 2045-2322
- Zeitschrift
- Scientific reports
- Schlüsselwörter
- 610 Medizin
- 610 Medical sciences
- Sprache
- eng
- Paginierung
- Art. 7111
- Datum der Veröffentlichung
- 2017
- Herausgeber
- Macmillan Publishers Limited, part of Springer Nature
- Herausgeber URL
- http://dx.doi.org/10.1038/s41598-017-07768-7
- Datum der Datenerfassung
- 2020
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Zugang
- Public
- Titel
- Compensatory vasodilator mechanisms in the ophthalmic artery of endothelial nitric oxide synthase gene knockout mice
- Ausgabe der Zeitschrift
- 7
Datenquelle: METADATA.UB
- Andere Metadatenquellen:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Nitric oxide (NO) generated by endothelial nitric oxide synthase (eNOS) plays an important role in the maintenance of ocular vascular homeostasis. Therefore, perturbations in vascular NO synthesis have been implicated in the pathogenesis of several ocular diseases. We recently reported that eNOS contributes significantly to vasodilation of the mouse ophthalmic artery. Interestingly, dilatory responses were also retained in eNOS gene-deficient mice (eNOS−/−), indicating inherent endothelial adaptive mechanism(s) that act as back-up systems in chronic absence of eNOS to preserve vasorelaxation. Thus, this study endeavoured to identify the compensatory mechanism(s) in the ophthalmic artery of eNOS−/− mice employing isolated arterial segments and pharmacological inhibitors <jats:italic>in vitro</jats:italic>. Endothelium removal virtually abolished acetylcholine (ACh)-induced vasodilation, suggesting an obligatory involvement of the endothelium in cholinergic control of vascular tone. However, non-NOS and non-cyclooxygenase components compensate for eNOS deficiency <jats:italic>via</jats:italic> endothelium-derived hyperpolarizing factors (EDHFs). Notably, arachidonic acid-derived metabolites of the 12-lipoxygenase pathway were key mediators in activating the inwardly rectifying potassium channels to compensate for chronic lack of eNOS. Conclusively, endothelium-dependent cholinergic responses of the ophthalmic artery in the eNOS−/− mice are largely preserved and, this vascular bed has the ability to compensate for the loss of normal vasodilator responses solely <jats:italic>via</jats:italic> EDHFs.</jats:p>
- Autoren
- Caroline Manicam
- Natalja Ginter
- Huige Li
- Ning Xia
- Evgeny Goloborodko
- Jenia Kouchek Zadeh
- Aytan Musayeva
- Norbert Pfeiffer
- Adrian Gericke
- DOI
- 10.1038/s41598-017-07768-7
- eISSN
- 2045-2322
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Scientific Reports
- Sprache
- en
- Artikelnummer
- 7111
- Online publication date
- 2017
- Status
- Published online
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1038/s41598-017-07768-7
- Datum der Datenerfassung
- 2022
- Titel
- Compensatory Vasodilator Mechanisms in the Ophthalmic Artery of Endothelial Nitric Oxide Synthase Gene Knockout Mice
- Ausgabe der Zeitschrift
- 7
Datenquelle: Crossref
- Author's licence
- CC-BY
- Autoren
- Caroline Manicam
- Natalja Ginter
- Huige Li
- Ning Xia
- Evgeny Goloborodko
- Jenia Kouchek Zadeh
- Aytan Musayeva
- Norbert Pfeiffer
- Adrian Gericke
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- DFG-OA-Publizieren (2012 - 2017)
- Resource version
- Published version
- DOI
- 10.1038/s41598-017-07768-7
- Funding acknowledgements
- DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 2045-2322
- Zeitschrift
- Scientific reports
- Schlüsselwörter
- 610 Medizin
- 610 Medical sciences
- Sprache
- eng
- Open access status
- Open Access
- Paginierung
- Art. 7111
- Datum der Veröffentlichung
- 2017
- Public URL
- https://openscience.ub.uni-mainz.de/handle/20.500.12030/7167
- Herausgeber
- Macmillan Publishers Limited, part of Springer Nature
- Herausgeber URL
- http://dx.doi.org/10.1038/s41598-017-07768-7
- Datum der Datenerfassung
- 2022
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2022
- Zugang
- Public
- Titel
- Compensatory vasodilator mechanisms in the ophthalmic artery of endothelial nitric oxide synthase gene knockout mice
- Ausgabe der Zeitschrift
- 7
Files
compensatory_vasodilator_mech-20220612163724820.pdf
Datenquelle: OPENSCIENCE.UB