Role of protein kinase C and Nox2-derived reactive oxygen species formation in the activation and maturation of dendritic cells by phorbol ester and lipopolysaccharide
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Judith Stein
- Sebastian Steven
- Matthias Bros
- Stephan Sudowe
- Michael Hausding
- Matthias Oelze
- Thomas Münzel
- Stephan Grabbe
- Angelika B Reske-Kunz
- Andreas Daiber
- Sammlungen
- metadata
- ISSN
- 1942-0994
- Zeitschrift
- Oxidative medicine and cellular longevity
- Schlüsselwörter
- 610 Medizin
- 610 Medical sciences
- Sprache
- eng
- Paginierung
- Art. 4157213
- Datum der Veröffentlichung
- 2017
- Herausgeber
- Landes Bioscience
- Herausgeber URL
- http://dx.doi.org/10.1155/2017/4157213
- Datum der Datenerfassung
- 2020
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Zugang
- Public
- Titel
- Role of protein kinase C and Nox2-derived reactive oxygen species formation in the activation and maturation of dendritic cells by phorbol ester and lipopolysaccharide
- Ausgabe der Zeitschrift
- 2017
Datenquelle: METADATA.UB
- Andere Metadatenquellen:
-
- Abstract
- <jats:p><jats:italic>Aims</jats:italic>. Activation/maturation of dendritic cells (DCs) plays a central role in adaptive immune responses by antigen processing and (cross-) activation of T cells. There is ongoing discussion on the role of reactive oxygen species (ROS) in these processes and with the present study we investigated this enigmatic pathway.<jats:italic>Methods and Results</jats:italic>. DCs were cultured from precursors in the bone marrow of mice (BM-DCs) and analyzed for ROS formation, maturation, and T cell stimulatory capacity upon stimulation with phorbol ester (PDBu) and lipopolysaccharide (LPS). LPS stimulation of BM-DCs caused maturation with moderate intracellular ROS formation, whereas PDBu treatment resulted in maturation with significant ROS formation. The NADPH oxidase inhibitors apocynin/VAS2870 and genetic gp91phox deletion both decreased the ROS signal in PDBu-stimulated BM-DCs without affecting maturation and T cell stimulatory capacity of BM-DCs. In contrast, the protein kinase C inhibitors chelerythrine/Gö6983 decreased PDBu-stimulated ROS formation in BM-DCs as well as maturation.<jats:italic>Conclusion</jats:italic>. Obviously Nox2-dependent ROS formation in BM-DCs is not always required for their maturation or T cell stimulatory potential. PDBu/LPS-triggered BM-DC maturation rather relies on phosphorylation cascades. Our results question the role of oxidative stress as an essential “danger signal” for BM-DC activation, although we cannot exclude contribution by other ROS sources.</jats:p>
- Autoren
- Judith Stein
- Sebastian Steven
- Matthias Bros
- Stephan Sudowe
- Michael Hausding
- Matthias Oelze
- Thomas Münzel
- Stephan Grabbe
- Angelika Reske-Kunz
- Andreas Daiber
- DOI
- 10.1155/2017/4157213
- eISSN
- 1942-0994
- ISSN
- 1942-0900
- Zeitschrift
- Oxidative Medicine and Cellular Longevity
- Sprache
- en
- Paginierung
- 1 - 12
- Datum der Veröffentlichung
- 2017
- Status
- Published
- Herausgeber
- Hindawi Limited
- Herausgeber URL
- http://dx.doi.org/10.1155/2017/4157213
- Datum der Datenerfassung
- 2017
- Titel
- Role of Protein Kinase C and Nox2-Derived Reactive Oxygen Species Formation in the Activation and Maturation of Dendritic Cells by Phorbol Ester and Lipopolysaccharide
- Ausgabe der Zeitschrift
- 2017
Datenquelle: Crossref
- Author's licence
- CC-BY
- Autoren
- Judith Stein
- Sebastian Steven
- Matthias Bros
- Stephan Sudowe
- Michael Hausding
- Matthias Oelze
- Thomas Münzel
- Stephan Grabbe
- Angelika B Reske-Kunz
- Andreas Daiber
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- DFG-OA-Publizieren (2012 - 2017)
- Resource version
- Published version
- DOI
- 10.1155/2017/4157213
- Funding acknowledgements
- DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 1942-0994
- Zeitschrift
- Oxidative medicine and cellular longevity
- Schlüsselwörter
- 610 Medizin
- 610 Medical sciences
- Sprache
- eng
- Open access status
- Open Access
- Paginierung
- Art. 4157213
- Datum der Veröffentlichung
- 2017
- Public URL
- https://openscience.ub.uni-mainz.de/handle/20.500.12030/7858
- Herausgeber
- Landes Bioscience
- Herausgeber URL
- http://dx.doi.org/10.1155/2017/4157213
- Datum der Datenerfassung
- 2022
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2022
- Zugang
- Public
- Titel
- Role of protein kinase C and Nox2-derived reactive oxygen species formation in the activation and maturation of dendritic cells by phorbol ester and lipopolysaccharide
- Ausgabe der Zeitschrift
- 2017
Files
role_of_protein_kinase_c_and_-20221005105350588.pdf
Datenquelle: OPENSCIENCE.UB