Fetuin-b, a liver-derived plasma protein is essential for fertilization

Autoren

Eileen Dietzel
Jennifer Wessling
Julia Floehr
Cora Schäfer
Silke Ensslen
Bernd Denecke
Benjamin Rösing
Joseph Neulen
Thomas Veitinger
Marc Spehr
Tanja Tropartz
René Tolba
Thomas Renné
Angela Egert
Hubert Schorle
Yuliya Gottenbusch
André Hildebrand
Irene Yiallouros
Walter Stöcker
Ralf Weiskirchen
Willi Jahnen-Dechent

DOI

10.1016/j.devcel.2013.03.001

ISSN

1534-5807

Ausgabe der Veröffentlichung

1

Zeitschrift

Developmental Cell

Sprache

en

Paginierung

106 - 112

Datum der Veröffentlichung

2013

Status

Published

Herausgeber

Elsevier BV

Herausgeber URL

http://dx.doi.org/10.1016/j.devcel.2013.03.001

Datum der Datenerfassung

2022

Titel

Fetuin-B, a Liver-Derived Plasma Protein Is Essential for Fertilization

Ausgabe der Zeitschrift

25

Datenquelle: Crossref

Abstract

The zona pellucida (ZP) is a glycoprotein matrix surrounding mammalian oocytes. Upon fertilization, ZP hardening prevents sperm from binding to and penetrating the ZP. Here, we report that targeted gene deletion of the liver-derived plasma protein fetuin-B causes premature ZP hardening and, consequently, female infertility. Transplanting fetuin-B-deficient ovaries into wild-type recipients restores fertility, indicating that plasma fetuin-B is necessary and sufficient for fertilization. In vitro fertilization of oocytes from fetuin-B-deficient mice only worked after rendering the ZP penetrable by laser perforation. Mechanistically, fetuin-B sustains fertility by inhibiting ovastacin, a cortical granula protease known to trigger ZP hardening. Thus, plasma fetuin-B is necessary to restrain protease activity and thereby maintain ZP permeability until after gamete fusion. These results also show that premature ZP hardening can cause infertility in mice.

Addresses

Helmholtz Institute for Biomedical Engineering, Biointerface Laboratory, 52074 Aachen, Germany.

Autoren

Eileen Dietzel
Jennifer Wessling
Julia Floehr
Cora Schäfer
Silke Ensslen
Bernd Denecke
Benjamin Rösing
Joseph Neulen
Thomas Veitinger
Marc Spehr
Tanja Tropartz
René Tolba
Thomas Renné
Angela Egert
Hubert Schorle
Yuliya Gottenbusch
André Hildebrand
Irene Yiallouros
Walter Stöcker
Ralf Weiskirchen
Willi Jahnen-Dechent

DOI

10.1016/j.devcel.2013.03.001

eISSN

1878-1551

Externe Identifier

PubMed Identifier: 23562279

Open access

false

ISSN

1534-5807

Ausgabe der Veröffentlichung

1

Zeitschrift

Developmental cell

Schlüsselwörter

Ovary
Oocytes
Zona Pellucida
Spermatozoa
Animals
Mice, Inbred C57BL
Mice
Infertility, Female
Metalloproteases
Recombinant Proteins
Embryo Transfer
Fertilization in Vitro
Cell Membrane Permeability
Gene Expression Regulation, Developmental
Enzyme Activation
Fertilization
Pregnancy
Female
Male
Embryo, Mammalian
Fetuin-B

Sprache

eng

Medium

Print-Electronic

Online publication date

2013

Paginierung

106 - 112

Datum der Veröffentlichung

2013

Status

Published

Datum der Datenerfassung

2013

Titel

Fetuin-B, a liver-derived plasma protein is essential for fertilization.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

25

Datenquelle: Europe PubMed Central

Abstract

The zona pellucida (ZP) is a glycoprotein matrix surrounding mammalian oocytes. Upon fertilization, ZP hardening prevents sperm from binding to and penetrating the ZP. Here, we report that targeted gene deletion of the liver-derived plasma protein fetuin-B causes premature ZP hardening and, consequently, female infertility. Transplanting fetuin-B-deficient ovaries into wild-type recipients restores fertility, indicating that plasma fetuin-B is necessary and sufficient for fertilization. In vitro fertilization of oocytes from fetuin-B-deficient mice only worked after rendering the ZP penetrable by laser perforation. Mechanistically, fetuin-B sustains fertility by inhibiting ovastacin, a cortical granula protease known to trigger ZP hardening. Thus, plasma fetuin-B is necessary to restrain protease activity and thereby maintain ZP permeability until after gamete fusion. These results also show that premature ZP hardening can cause infertility in mice.

Date of acceptance

2013

Autoren

Eileen Dietzel
Jennifer Wessling
Julia Floehr
Cora Schäfer
Silke Ensslen
Bernd Denecke
Benjamin Rösing
Joseph Neulen
Thomas Veitinger
Marc Spehr
Tanja Tropartz
René Tolba
Thomas Renné
Angela Egert
Hubert Schorle
Yuliya Gottenbusch
André Hildebrand
Irene Yiallouros
Walter Stöcker
Ralf Weiskirchen
Willi Jahnen-Dechent

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/23562279

DOI

10.1016/j.devcel.2013.03.001

eISSN

1878-1551

Ausgabe der Veröffentlichung

1

Zeitschrift

Dev Cell

Schlüsselwörter

Animals
Cell Membrane Permeability
Embryo Transfer
Embryo, Mammalian
Enzyme Activation
Female
Fertilization
Fertilization in Vitro
Fetuin-B
Gene Expression Regulation, Developmental
Infertility, Female
Male
Metalloproteases
Mice
Mice, Inbred C57BL
Oocytes
Ovary
Pregnancy
Recombinant Proteins
Spermatozoa
Zona Pellucida

Sprache

eng

Country

United States

Paginierung

106 - 112

PII

S1534-5807(13)00132-9

Datum der Veröffentlichung

2013

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2013

Titel

Fetuin-B, a liver-derived plasma protein is essential for fertilization.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

25

Datenquelle: PubMed

Fetuin-b, a liver-derived plasma protein is essential for fertilization

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