Oxacyclododecindione, a novel inhibitor of il-4 signaling from exserohilum rostratum

Autoren

Gerhard Erkel
Hanane Belahmer
Annegret Serwe
Timm Anke
Horst Kunz
Heinz Kolshorn
Johannes Liermann
Till Opatz

Autoren-URL

https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000256946900002&DestLinkType=FullRecord&DestApp=WOS_CPL

DOI

10.1038/ja.2008.40

Externe Identifier

Clarivate Analytics Document Solution ID: 316JY
PubMed Identifier: 18653993

ISSN

0021-8820

Ausgabe der Veröffentlichung

5

Zeitschrift

JOURNAL OF ANTIBIOTICS

Schlüsselwörter

exserohilum
interleukin-4 signaling
inhibitor
new metabolite

Paginierung

285 - 290

Datum der Veröffentlichung

2008

Status

Published

Titel

Oxacyclododecindione, a novel inhibitor of IL-4 signaling from <i>Exserohilum rostratum</i>

Sub types

Article

Ausgabe der Zeitschrift

61

Datenquelle: Web of Science (Lite)

Autoren

Gerhard Erkel
Hanane Belahmer
Annegret Serwe
Timm Anke
Horst Kunz
Heinz Kolshorn
Johannes Liermann
Till Opatz

DOI

10.1038/ja.2008.40

eISSN

1881-1469

ISSN

0021-8820

Ausgabe der Veröffentlichung

5

Zeitschrift

The Journal of Antibiotics

Sprache

en

Paginierung

285 - 290

Datum der Veröffentlichung

2008

Status

Published

Herausgeber

Springer Science and Business Media LLC

Herausgeber URL

http://dx.doi.org/10.1038/ja.2008.40

Datum der Datenerfassung

2023

Titel

Oxacyclododecindione, a Novel Inhibitor of IL-4 Signaling from Exserohilum rostratum

Ausgabe der Zeitschrift

61

Datenquelle: Crossref

Abstract

In a screening program for new metabolites from fungi inhibiting the IL-4 mediated signal transduction, a novel chlorinated macrocyclic lactone, designated as oxacyclododecindione, was isolated from fermentations of the imperfect fungus Exserohilum rostratum. The structure was determined by a combination of spectroscopic techniques. Oxacyclododecindione inhibits the IL-4 induced expression of the reporter gene secreted alkaline phosphatase (SEAP) in transiently transfected HepG2 cells with IC50 values of 20-25 ng/ml (54-67.5 nM). Studies on the mode of action of the compound revealed that the inhibition of the IL-4 dependent signaling pathway is caused by blocking the binding of the activated STAT6 transcription factors to the DNA binding site without inhibiting tyrosine phosphorylation. The compound has no antibacterial or antifungal activity.

Addresses

Department of Biotechnology, University of Kaiserslautern, Kaiserslautern, Germany. erkel@ibwf.de

Autoren

Gerhard Erkel
Hanane Belahmer
Annegret Serwe
Timm Anke
Horst Kunz
Heinz Kolshorn
Johannes Liermann
Till Opatz

DOI

10.1038/ja.2008.40

eISSN

1881-1469

Externe Identifier

PubMed Identifier: 18653993

Open access

false

ISSN

0021-8820

Ausgabe der Veröffentlichung

5

Zeitschrift

The Journal of antibiotics

Schlüsselwörter

Cell Line, Tumor
Humans
Mitosporic Fungi
Macrocyclic Compounds
Interleukin-4
Blotting, Western
Magnetic Resonance Spectroscopy
Spectrometry, Mass, Electrospray Ionization
Transfection
Signal Transduction
Gene Expression
Fermentation
Structure-Activity Relationship
STAT6 Transcription Factor

Sprache

eng

Medium

Print

Paginierung

285 - 290

Datum der Veröffentlichung

2008

Status

Published

Datum der Datenerfassung

2008

Titel

Oxacyclododecindione, a novel inhibitor of IL-4 signaling from Exserohilum rostratum.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

61

Datenquelle: Europe PubMed Central

Abstract

In a screening program for new metabolites from fungi inhibiting the IL-4 mediated signal transduction, a novel chlorinated macrocyclic lactone, designated as oxacyclododecindione, was isolated from fermentations of the imperfect fungus Exserohilum rostratum. The structure was determined by a combination of spectroscopic techniques. Oxacyclododecindione inhibits the IL-4 induced expression of the reporter gene secreted alkaline phosphatase (SEAP) in transiently transfected HepG2 cells with IC50 values of 20-25 ng/ml (54-67.5 nM). Studies on the mode of action of the compound revealed that the inhibition of the IL-4 dependent signaling pathway is caused by blocking the binding of the activated STAT6 transcription factors to the DNA binding site without inhibiting tyrosine phosphorylation. The compound has no antibacterial or antifungal activity.

Autoren

Gerhard Erkel
Hanane Belahmer
Annegret Serwe
Timm Anke
Horst Kunz
Heinz Kolshorn
Johannes Liermann
Till Opatz

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/18653993

DOI

10.1038/ja.2008.40

ISSN

0021-8820

Ausgabe der Veröffentlichung

5

Zeitschrift

J Antibiot (Tokyo)

Schlüsselwörter

Blotting, Western
Cell Line, Tumor
Fermentation
Gene Expression
Humans
Interleukin-4
Macrocyclic Compounds
Magnetic Resonance Spectroscopy
Mitosporic Fungi
STAT6 Transcription Factor
Signal Transduction
Spectrometry, Mass, Electrospray Ionization
Structure-Activity Relationship
Transfection

Sprache

eng

Country

England

Paginierung

285 - 290

PII

JST.JSTAGE/antibiotics/61.285

Datum der Veröffentlichung

2008

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2008

Titel

Oxacyclododecindione, a novel inhibitor of IL-4 signaling from Exserohilum rostratum.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

61

Datenquelle: PubMed

Oxacyclododecindione, a novel inhibitor of il-4 signaling from exserohilum rostratum

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