Establishing a novel single-copy primer-internal intron-spanning PCR (spiPCR) procedure for the direct detection of gene doping

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Thomas Beiter
  • Martina Zimmermann
  • Annunziata Fragasso
  • Sorin Armeanu
  • Ulrich M Lauer
  • Michael Bitzer
  • Hua Su
  • William L Young
  • Andreas M Niess
  • Perikles Simon
Sammlungen
  • metadata
ISSN
1077-5552
Zeitschrift
Exercise immunology review
Schlüsselwörter
  • 796 Sport
  • 796 Athletic and outdoor sports and games
Sprache
eng
Paginierung
Seiten: 73 - 85
Datum der Veröffentlichung
2008
Datum der Datenerfassung
2020
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Zugang
Public
Titel
Establishing a novel single-copy primer-internal intron-spanning PCR (spiPCR) procedure for the direct detection of gene doping
Ausgabe der Zeitschrift
14

Datenquelle: METADATA.UB

Andere Metadatenquellen:
Autoren
  • Thomas Beiter
  • Martina Zimmermann
  • Annunziata Fragasso
  • Sorin Armeanu
  • Ulrich M Lauer
  • Michael Bitzer
  • Hua Su
  • William L Young
  • Andreas M Niess
  • Perikles Simon
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000261860800007&DestLinkType=FullRecord&DestApp=WOS_CPL
Externe Identifier
  • Clarivate Analytics Document Solution ID: 386CV
  • PubMed Identifier: 19203085
ISSN
1077-5552
Zeitschrift
EXERCISE IMMUNOLOGY REVIEW
Schlüsselwörter
  • Gene doping
  • gene therapy
  • gene transfer
  • direct detection
  • spiPCR
  • transgenic DNA
Paginierung
73 - 85
Datum der Veröffentlichung
2008
Status
Published
Titel
Establishing a novel single-copy primer-internal intron-spanning PCR (spiPCR) procedure for the direct detection of gene doping
Sub types
  • Review
Ausgabe der Zeitschrift
14

Datenquelle: Web of Science (Lite)

Abstract
So far, the abuse of gene transfer technology in sport, so-called gene doping, is undetectable. However, recent studies in somatic gene therapy indicate that long-term presence of transgenic DNA (tDNA) following various gene transfer protocols can be found in DNA isolated from whole blood using conventional PCR protocols. Application of these protocols for the direct detection of gene doping would require almost complete knowledge about the sequence of the genetic information that has been transferred. Here, we develop and describe the novel single-copy primer-internal intron-spanning PCR (spiPCR) procedure that overcomes this difficulty. Apart from the interesting perspectives that this spiPCR procedure offers in the fight against gene doping, this technology could also be of interest in biodistribution and biosafety studies for gene therapeutic applications.
Addresses
  • Department of Sports Medicine, Medical Clinic, University of Tüebingen, Germany.
Autoren
  • Thomas Beiter
  • Martina Zimmermann
  • Annunziata Fragasso
  • Sorin Armeanu
  • Ulrich M Lauer
  • Michael Bitzer
  • Hua Su
  • William L Young
  • Andreas M Niess
  • Perikles Simon
eISSN
2959-9555
Externe Identifier
  • PubMed Identifier: 19203085
Open access
false
ISSN
1077-5552
Zeitschrift
Exercise immunology review
Schlüsselwörter
  • Humans
  • Vascular Endothelial Growth Factor D
  • Erythropoietin
  • DNA
  • Polymerase Chain Reaction
  • Transgenes
  • Doping in Sports
  • Genetic Therapy
Sprache
eng
Medium
Print
Paginierung
73 - 85
Datum der Veröffentlichung
2008
Status
Published
Datum der Datenerfassung
2009
Titel
Establishing a novel single-copy primer-internal intron-spanning PCR (spiPCR) procedure for the direct detection of gene doping.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
14

Datenquelle: Europe PubMed Central

Abstract
So far, the abuse of gene transfer technology in sport, so-called gene doping, is undetectable. However, recent studies in somatic gene therapy indicate that long-term presence of transgenic DNA (tDNA) following various gene transfer protocols can be found in DNA isolated from whole blood using conventional PCR protocols. Application of these protocols for the direct detection of gene doping would require almost complete knowledge about the sequence of the genetic information that has been transferred. Here, we develop and describe the novel single-copy primer-internal intron-spanning PCR (spiPCR) procedure that overcomes this difficulty. Apart from the interesting perspectives that this spiPCR procedure offers in the fight against gene doping, this technology could also be of interest in biodistribution and biosafety studies for gene therapeutic applications.
Autoren
  • Thomas Beiter
  • Martina Zimmermann
  • Annunziata Fragasso
  • Sorin Armeanu
  • Ulrich M Lauer
  • Michael Bitzer
  • Hua Su
  • William L Young
  • Andreas M Niess
  • Perikles Simon
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/19203085
ISSN
1077-5552
Zeitschrift
Exerc Immunol Rev
Schlüsselwörter
  • DNA
  • Doping in Sports
  • Erythropoietin
  • Genetic Therapy
  • Humans
  • Polymerase Chain Reaction
  • Transgenes
  • Vascular Endothelial Growth Factor D
Sprache
eng
Country
Germany
Paginierung
73 - 85
Datum der Veröffentlichung
2008
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2009
Titel
Establishing a novel single-copy primer-internal intron-spanning PCR (spiPCR) procedure for the direct detection of gene doping.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
14

Datenquelle: PubMed

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