Caki-1 cells represent an in vitro model system for studying the human proximal tubule epithelium
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Natalie Glube
- Andreas Giessl
- Uwe Wolfrum
- Peter Langguth
- Sammlungen
- metadata
- ISSN
- 1660-2129
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Nephron. Experimental nephrology
- Schlüsselwörter
- 610 Medizin
- 610 Medical sciences
- Sprache
- eng
- Paginierung
- Seiten: e47 - e56
- Datum der Veröffentlichung
- 2007
- Herausgeber
- Karger
- Herausgeber URL
- http://dx.doi.org/10.1159/000107804
- Datum der Datenerfassung
- 2020
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Zugang
- Public
- Titel
- Caki-1 cells represent an in vitro model system for studying the human proximal tubule epithelium
- Ausgabe der Zeitschrift
- 107
Datenquelle: METADATA.UB
- Andere Metadatenquellen:
-
- Autoren
- Natalie Glube
- Andreas Giessl
- Uwe Wolfrum
- Peter Langguth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000249722200002&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1159/000107804
- Externe Identifier
- Clarivate Analytics Document Solution ID: 214FU
- PubMed Identifier: 17804913
- ISSN
- 1660-2129
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- NEPHRON EXPERIMENTAL NEPHROLOGY
- Schlüsselwörter
- Caki-1
- proximal tubule
- electron microscopy
- differentiation
- human kidney
- Paginierung
- E47 - E56
- Datum der Veröffentlichung
- 2007
- Status
- Published
- Titel
- Caki-1 cells represent an in vitro model system for studying the human proximal tubule epithelium
- Sub types
- Article
- Ausgabe der Zeitschrift
- 107
Datenquelle: Web of Science (Lite)
- Abstract
- <jats:p><i>Background/Aims:</i> The human proximal tubule (PT) epithelium is distinguished from other nephron segments via several unique characteristics. Studies assessing PT epithelium increasingly employ cell lines, bypassing the complexity of primary cell cultures. However, few human model systems exist for studying PT cells in vitro. The current work involves an intensive characterization of Caki-1 cells, a commercially available human renal cell line. <i>Methods:</i> Caki-1 cells were validated as a representative model system for PT cell research via morphological, physiological and biochemical investigations including light and transmission electron microscopy, transepithelial electrical resistance (TER) measurements and the detection of PT markers. <i>Results:</i> Morphologically, these cells form a polarized monolayer with apical located microvilli and multiple mitochondria per cell. Low TER ranging from 2 to 28 Ω cm<sup>2</sup> was determined for Caki-1 cells, characteristic of the ‘leaky’ PT epithelium in vivo. Expression of the PT markers: NHE3, GGT, DPP IV, APM and AP were present in Caki-1 cells. Two epithelial markers, E-cadherin and Na<sup>+</sup>/K<sup>+</sup>-ATPase, were additionally observed. <i>Conclusion:</i> The current work is a concise summary which confirms that Caki-1 cells represent well-differentiated polarized PT cells in vitro, regardless of its cancerous origin and multiple passaging. They prove to be a significant contribution to the field of PT research.</jats:p>
- Autoren
- Natalie Glube
- Andreas Giessl
- Uwe Wolfrum
- Peter Langguth
- DOI
- 10.1159/000107804
- eISSN
- 1660-2129
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Nephron Experimental Nephrology
- Sprache
- en
- Online publication date
- 2007
- Paginierung
- e47 - e56
- Status
- Published online
- Herausgeber
- S. Karger AG
- Herausgeber URL
- http://dx.doi.org/10.1159/000107804
- Datum der Datenerfassung
- 2022
- Titel
- Caki-1 Cells Represent an in vitro Model System for Studying the Human Proximal Tubule Epithelium
- Ausgabe der Zeitschrift
- 107
Datenquelle: Crossref
- Abstract
- <h4>Background/aims</h4>The human proximal tubule (PT) epithelium is distinguished from other nephron segments via several unique characteristics. Studies assessing PT epithelium increasingly employ cell lines, bypassing the complexity of primary cell cultures. However, few human model systems exist for studying PT cells in vitro. The current work involves an intensive characterization of Caki-1 cells, a commercially available human renal cell line.<h4>Methods</h4>Caki-1 cells were validated as a representative model system for PT cell research via morphological, physiological and biochemical investigations including light and transmission electron microscopy, transepithelial electrical resistance (TER) measurements and the detection of PT markers.<h4>Results</h4>Morphologically, these cells form a polarized monolayer with apical located microvilli and multiple mitochondria per cell. Low TER ranging from 2 to 28 Omega cm(2) was determined for Caki-1 cells, characteristic of the 'leaky' PT epithelium in vivo. Expression of the PT markers: NHE3, GGT, DPP IV, APM and AP were present in Caki-1 cells. Two epithelial markers, E-cadherin and Na(+)/K(+)-ATPase, were additionally observed.<h4>Conclusion</h4>The current work is a concise summary which confirms that Caki-1 cells represent well-differentiated polarized PT cells in vitro, regardless of its cancerous origin and multiple passaging. They prove to be a significant contribution to the field of PT research.
- Addresses
- Institute of Pharmacy, Johannes Gutenberg University, Department of Pharmaceutical Technology, Mainz, Germany.
- Autoren
- Natalie Glube
- Andreas Giessl
- Uwe Wolfrum
- Peter Langguth
- DOI
- 10.1159/000107804
- eISSN
- 1660-2129
- Externe Identifier
- PubMed Identifier: 17804913
- Open access
- false
- ISSN
- 1660-8151
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Nephron. Experimental nephrology
- Schlüsselwörter
- Kidney Tubules, Proximal
- Cells, Cultured
- Cell Line, Tumor
- Caco-2 Cells
- Epithelial Cells
- Humans
- Kidney Neoplasms
- Biomarkers
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2007
- Paginierung
- e47 - e56
- Datum der Veröffentlichung
- 2007
- Status
- Published
- Datum der Datenerfassung
- 2007
- Titel
- Caki-1 cells represent an in vitro model system for studying the human proximal tubule epithelium.
- Sub types
- Research Support, Non-U.S. Gov't
- Validation Study
- Journal Article
- Ausgabe der Zeitschrift
- 107
Datenquelle: Europe PubMed Central
- Abstract
- BACKGROUND/AIMS: The human proximal tubule (PT) epithelium is distinguished from other nephron segments via several unique characteristics. Studies assessing PT epithelium increasingly employ cell lines, bypassing the complexity of primary cell cultures. However, few human model systems exist for studying PT cells in vitro. The current work involves an intensive characterization of Caki-1 cells, a commercially available human renal cell line. METHODS: Caki-1 cells were validated as a representative model system for PT cell research via morphological, physiological and biochemical investigations including light and transmission electron microscopy, transepithelial electrical resistance (TER) measurements and the detection of PT markers. RESULTS: Morphologically, these cells form a polarized monolayer with apical located microvilli and multiple mitochondria per cell. Low TER ranging from 2 to 28 Omega cm(2) was determined for Caki-1 cells, characteristic of the 'leaky' PT epithelium in vivo. Expression of the PT markers: NHE3, GGT, DPP IV, APM and AP were present in Caki-1 cells. Two epithelial markers, E-cadherin and Na(+)/K(+)-ATPase, were additionally observed. CONCLUSION: The current work is a concise summary which confirms that Caki-1 cells represent well-differentiated polarized PT cells in vitro, regardless of its cancerous origin and multiple passaging. They prove to be a significant contribution to the field of PT research.
- Date of acceptance
- 2007
- Autoren
- Natalie Glube
- Andreas Giessl
- Uwe Wolfrum
- Peter Langguth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/17804913
- DOI
- 10.1159/000107804
- eISSN
- 1660-2129
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- Nephron Exp Nephrol
- Schlüsselwörter
- Biomarkers
- Caco-2 Cells
- Cell Line, Tumor
- Cells, Cultured
- Epithelial Cells
- Humans
- Kidney Neoplasms
- Kidney Tubules, Proximal
- Sprache
- eng
- Country
- Switzerland
- Paginierung
- e47 - e56
- PII
- 000107804
- Datum der Veröffentlichung
- 2007
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2007
- Titel
- Caki-1 cells represent an in vitro model system for studying the human proximal tubule epithelium.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Validation Study
- Ausgabe der Zeitschrift
- 107
Datenquelle: PubMed
- Beziehungen:
- Eigentum von