Optimized basic conditions are essential for successful siRNA transfection into primary endothelial cells

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Andrea Nolte
  • Claudia Raabe
  • Tobias Walker
  • Perikles Simon
  • Gerhard Ziemer
  • Hans Peter Wendel
Sammlungen
  • metadata
ISSN
1545-4576
Ausgabe der Veröffentlichung
2
Zeitschrift
Oligonucleotides
Schlüsselwörter
  • 796 Sport
  • 796 Athletic and outdoor sports and games
Sprache
eng
Paginierung
Seiten: 141 - 150
Datum der Veröffentlichung
2009
Herausgeber URL
http://dx.doi.org/10.1089/oli.2009.0182
Datum der Datenerfassung
2020
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Zugang
Public
Titel
Optimized basic conditions are essential for successful siRNA transfection into primary endothelial cells
Ausgabe der Zeitschrift
19

Datenquelle: METADATA.UB

Andere Metadatenquellen:
Autoren
  • Andrea Nolte
  • Claudia Raabe
  • Tobias Walker
  • Perikles Simon
  • Gerhard Ziemer
  • Hans Peter Wendel
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000266730100005&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1089/oli.2009.0182
eISSN
1557-8526
Externe Identifier
  • Clarivate Analytics Document Solution ID: 455BI
  • PubMed Identifier: 19441891
ISSN
1545-4576
Ausgabe der Veröffentlichung
2
Zeitschrift
OLIGONUCLEOTIDES
Paginierung
141 - 149
Datum der Veröffentlichung
2009
Status
Published
Titel
Optimized Basic Conditions Are Essential for Successful siRNA Transfection into Primary Endothelial Cells
Sub types
  • Article
Ausgabe der Zeitschrift
19

Datenquelle: Web of Science (Lite)

Autoren
  • Andrea Nolte
  • Claudia Raabe
  • Tobias Walker
  • Perikles Simon
  • Gerhard Ziemer
  • Hans Peter Wendel
DOI
10.1089/oli.2009.0182
eISSN
1557-8526
ISSN
1545-4576
Ausgabe der Veröffentlichung
2
Zeitschrift
Oligonucleotides
Sprache
en
Paginierung
141 - 150
Datum der Veröffentlichung
2009
Status
Published
Herausgeber
Mary Ann Liebert Inc
Herausgeber URL
http://dx.doi.org/10.1089/oli.2009.0182
Datum der Datenerfassung
2021
Titel
Optimized Basic Conditions Are Essential for Successful siRNA Transfection into Primary Endothelial Cells
Ausgabe der Zeitschrift
19

Datenquelle: Crossref

Abstract
RNA interference (RNAi) is a powerful technique in basic research and has a high potential for therapeutic applications. To realize its clinical applicability, introduction of short double-stranded RNA (dsRNA) has to be carried out under physiological conditions. This study evaluates two cationic liposomal transfection reagents on the efficiency of successful silencing of primary human endothelial cells. Transfection efficiency was investigated under different conditions, for example different media during transfection, duration of transfection, siRNA concentration, and the use of serum and antibiotics. Viability after transfection was examined by CASY and MTT assay. Interferon response was examined by real-time PCR. First we revealed that transfection carried out in the presence of serum and antibiotics caused good knockdown results only by the use of the novel lipid cationic transfection reagent. Both lipid cations had slightly the same transfection efficiency over the range of 10-150 nM siRNA concentration. Examination of interferon response showed increasing OAS1 and STAT1 expression, but not as high as if the transfections were carried out with synthetic polyinosinic-polycytidylic acid double-stranded RNA (poly[IC]). The optimized combination of basic conditions for transfection significantly enhanced the efficiency of the siRNA-mediated knockdown, without causing toxicity or stimulation of the interferon pathway.
Addresses
  • Department of Thoracic, Cardiac, and Vascular Surgery, Division of Sports Medicine, University Hospital Tübingen, Tübingen, Germany.
Autoren
  • Andrea Nolte
  • Claudia Raabe
  • Tobias Walker
  • Perikles Simon
  • Gerhard Ziemer
  • Hans Peter Wendel
DOI
10.1089/oli.2009.0182
eISSN
1557-8526
Externe Identifier
  • PubMed Identifier: 19441891
Open access
false
ISSN
1545-4576
Ausgabe der Veröffentlichung
2
Zeitschrift
Oligonucleotides
Schlüsselwörter
  • Cells, Cultured
  • Endothelial Cells
  • Humans
  • Lipids
  • E-Selectin
  • Interferons
  • RNA, Small Interfering
  • Liposomes
  • Indicators and Reagents
  • Transfection
Sprache
eng
Medium
Print
Paginierung
141 - 150
Datum der Veröffentlichung
2009
Status
Published
Datum der Datenerfassung
2009
Titel
Optimized basic conditions are essential for successful siRNA transfection into primary endothelial cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
19

Datenquelle: Europe PubMed Central

Abstract
RNA interference (RNAi) is a powerful technique in basic research and has a high potential for therapeutic applications. To realize its clinical applicability, introduction of short double-stranded RNA (dsRNA) has to be carried out under physiological conditions. This study evaluates two cationic liposomal transfection reagents on the efficiency of successful silencing of primary human endothelial cells. Transfection efficiency was investigated under different conditions, for example different media during transfection, duration of transfection, siRNA concentration, and the use of serum and antibiotics. Viability after transfection was examined by CASY and MTT assay. Interferon response was examined by real-time PCR. First we revealed that transfection carried out in the presence of serum and antibiotics caused good knockdown results only by the use of the novel lipid cationic transfection reagent. Both lipid cations had slightly the same transfection efficiency over the range of 10-150 nM siRNA concentration. Examination of interferon response showed increasing OAS1 and STAT1 expression, but not as high as if the transfections were carried out with synthetic polyinosinic-polycytidylic acid double-stranded RNA (poly[IC]). The optimized combination of basic conditions for transfection significantly enhanced the efficiency of the siRNA-mediated knockdown, without causing toxicity or stimulation of the interferon pathway.
Autoren
  • Andrea Nolte
  • Claudia Raabe
  • Tobias Walker
  • Perikles Simon
  • Gerhard Ziemer
  • Hans Peter Wendel
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/19441891
DOI
10.1089/oli.2009.0182
eISSN
1557-8526
Ausgabe der Veröffentlichung
2
Zeitschrift
Oligonucleotides
Schlüsselwörter
  • Cells, Cultured
  • E-Selectin
  • Endothelial Cells
  • Humans
  • Indicators and Reagents
  • Interferons
  • Lipids
  • Liposomes
  • RNA, Small Interfering
  • Transfection
Sprache
eng
Country
United States
Paginierung
141 - 150
Datum der Veröffentlichung
2009
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2009
Titel
Optimized basic conditions are essential for successful siRNA transfection into primary endothelial cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
19

Datenquelle: PubMed

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