Tyramine actions on Drosophila flight behavior are affected by a glial dehydrogenase/reductase

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Stefanie Ryglewski
  • Carsten Duch
  • Benjamin Altenhein
Sammlungen
  • metadata
ISSN
1662-5137
Zeitschrift
Frontiers in systems neuroscience
Schlüsselwörter
  • 570 Biowissenschaften
  • 570 Life sciences
Sprache
eng
Paginierung
Art. 68
Datum der Veröffentlichung
2017
Herausgeber
Frontiers Research Foundation
Herausgeber URL
http://dx.doi.org/10.3389/fnsys.2017.00068
Datum der Datenerfassung
2020
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Zugang
Public
Titel
Tyramine actions on Drosophila flight behavior are affected by a glial dehydrogenase/reductase
Ausgabe der Zeitschrift
11

Datenquelle: METADATA.UB

Andere Metadatenquellen:
Autoren
  • Stefanie Ryglewski
  • Carsten Duch
  • Benjamin Altenhein
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000411947800001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.3389/fnsys.2017.00068
Externe Identifier
  • Clarivate Analytics Document Solution ID: FI4NK
  • PubMed Identifier: 29021745
ISSN
1662-5137
Zeitschrift
FRONTIERS IN SYSTEMS NEUROSCIENCE
Schlüsselwörter
  • Drosophila
  • biogenic amine
  • tyramine
  • flight
  • modulation
  • glia
Artikelnummer
ARTN 68
Datum der Veröffentlichung
2017
Status
Published
Titel
Tyramine Actions on <i>Drosophila</i> Flight Behavior Are Affected by a Glial Dehydrogenase/Reductase
Sub types
  • Article
Ausgabe der Zeitschrift
11

Datenquelle: Web of Science (Lite)

Autoren
  • Stefanie Ryglewski
  • Carsten Duch
  • Benjamin Altenhein
DOI
10.3389/fnsys.2017.00068
eISSN
1662-5137
Zeitschrift
Frontiers in Systems Neuroscience
Online publication date
2017
Status
Published online
Herausgeber
Frontiers Media SA
Herausgeber URL
http://dx.doi.org/10.3389/fnsys.2017.00068
Datum der Datenerfassung
2022
Titel
Tyramine Actions on Drosophila Flight Behavior Are Affected by a Glial Dehydrogenase/Reductase
Ausgabe der Zeitschrift
11

Datenquelle: Crossref

Abstract
The biogenic amines octopamine (OA) and tyramine (TA) modulate insect motor behavior in an antagonistic manner. OA generally enhances locomotor behaviors such as <i>Drosophila</i> larval crawling and flight, whereas TA decreases locomotor activity. However, the mechanisms and cellular targets of TA modulation of locomotor activity are incompletely understood. This study combines immunocytochemistry, genetics and flight behavioral assays in the <i>Drosophila</i> model system to test the role of a candidate enzyme for TA catabolism, named Nazgul (Naz), in flight motor behavioral control. We hypothesize that the dehydrogenase/reductase Naz represents a critical step in TA catabolism. Immunocytochemistry reveals that Naz is localized to a subset of Repo positive glial cells with cell bodies along the motor neuropil borders and numerous positive Naz arborizations extending into the synaptic flight motor neuropil. RNAi knock down of Naz in Repo positive glial cells reduces Naz protein level below detection level by Western blotting. The resulting consequence is a reduction in flight durations, thus mimicking known motor behavioral phenotypes as resulting from increased TA levels. In accord with the interpretation that reduced TA degradation by Naz results in increased TA levels in the flight motor neuropil, the motor behavioral phenotype can be rescued by blocking TA receptors. Our findings indicate that TA modulates flight motor behavior by acting on central circuitry and that TA is normally taken up from the central motor neuropil by Repo-positive glial cells, desaminated and further degraded by Naz.
Addresses
  • Institute of Developmental Biology and Neurobiology, Johannes Gutenberg-Universität Mainz, Mainz, Germany.
Autoren
  • Stefanie Ryglewski
  • Carsten Duch
  • Benjamin Altenhein
DOI
10.3389/fnsys.2017.00068
eISSN
1662-5137
Externe Identifier
  • PubMed Identifier: 29021745
  • PubMed Central ID: PMC5624129
Funding acknowledgements
  • Deutsche Forschungsgemeinschaft: Du-331/6-1, Ry 117/3-1
Open access
true
ISSN
1662-5137
Zeitschrift
Frontiers in systems neuroscience
Sprache
eng
Medium
Electronic-eCollection
Online publication date
2017
Open access status
Open Access
Paginierung
68
Datum der Veröffentlichung
2017
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2017
Titel
Tyramine Actions on <i>Drosophila</i> Flight Behavior Are Affected by a Glial Dehydrogenase/Reductase.
Sub types
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
11

Files

https://www.frontiersin.org/articles/10.3389/fnsys.2017.00068/pdf https://europepmc.org/articles/PMC5624129?pdf=render

Datenquelle: Europe PubMed Central

Abstract
The biogenic amines octopamine (OA) and tyramine (TA) modulate insect motor behavior in an antagonistic manner. OA generally enhances locomotor behaviors such as Drosophila larval crawling and flight, whereas TA decreases locomotor activity. However, the mechanisms and cellular targets of TA modulation of locomotor activity are incompletely understood. This study combines immunocytochemistry, genetics and flight behavioral assays in the Drosophila model system to test the role of a candidate enzyme for TA catabolism, named Nazgul (Naz), in flight motor behavioral control. We hypothesize that the dehydrogenase/reductase Naz represents a critical step in TA catabolism. Immunocytochemistry reveals that Naz is localized to a subset of Repo positive glial cells with cell bodies along the motor neuropil borders and numerous positive Naz arborizations extending into the synaptic flight motor neuropil. RNAi knock down of Naz in Repo positive glial cells reduces Naz protein level below detection level by Western blotting. The resulting consequence is a reduction in flight durations, thus mimicking known motor behavioral phenotypes as resulting from increased TA levels. In accord with the interpretation that reduced TA degradation by Naz results in increased TA levels in the flight motor neuropil, the motor behavioral phenotype can be rescued by blocking TA receptors. Our findings indicate that TA modulates flight motor behavior by acting on central circuitry and that TA is normally taken up from the central motor neuropil by Repo-positive glial cells, desaminated and further degraded by Naz.
Date of acceptance
2017
Autoren
  • Stefanie Ryglewski
  • Carsten Duch
  • Benjamin Altenhein
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/29021745
DOI
10.3389/fnsys.2017.00068
Externe Identifier
  • PubMed Central ID: PMC5624129
ISSN
1662-5137
Zeitschrift
Front Syst Neurosci
Schlüsselwörter
  • Drosophila
  • biogenic amine
  • flight
  • glia
  • modulation
  • tyramine
Sprache
eng
Country
Switzerland
Paginierung
68
Datum der Veröffentlichung
2017
Status
Published online
Titel
Tyramine Actions on Drosophila Flight Behavior Are Affected by a Glial Dehydrogenase/Reductase.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
11

Datenquelle: PubMed

Author's licence
CC-BY
Autoren
  • Stefanie Ryglewski
  • Carsten Duch
  • Benjamin Altenhein
Hosting institution
Universitätsbibliothek Mainz
Sammlungen
  • DFG-OA-Publizieren (2012 - 2017)
Resource version
Published version
DOI
10.3389/fnsys.2017.00068
Funding acknowledgements
  • DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin
File(s) embargoed
false
Open access
true
ISSN
1662-5137
Zeitschrift
Frontiers in systems neuroscience
Schlüsselwörter
  • 570 Biowissenschaften
  • 570 Life sciences
Sprache
eng
Open access status
Open Access
Paginierung
Art. 68
Datum der Veröffentlichung
2017
Public URL
https://openscience.ub.uni-mainz.de/handle/20.500.12030/8067
Herausgeber
Frontiers Research Foundation
Herausgeber URL
http://dx.doi.org/10.3389/fnsys.2017.00068
Datum der Datenerfassung
2022
Datum, an dem der Datensatz öffentlich gemacht wurde
2022
Zugang
Public
Titel
Tyramine actions on Drosophila flight behavior are affected by a glial dehydrogenase/reductase
Ausgabe der Zeitschrift
11

Files

tyramine_actions_on_drosophil-20220925164356754.pdf

Datenquelle: OPENSCIENCE.UB

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