Lipid Bilayer Interactions of Peptidic Supramolecular Polymers and Their Impact on Membrane Permeability and Stability
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Stefanie Pannwitt
- Jonas Kaltbeitzel
- Patrick Ahlers
- Daniel Spitzer
- Nadja Hellmann
- Frank Depoix
- Pol Besenius
- Dirk Schneider
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000537147200008&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1021/acs.biochem.0c00114
- Externe Identifier
- Clarivate Analytics Document Solution ID: LT5YU
- PubMed Identifier: 32320213
- ISSN
- 0006-2960
- Ausgabe der Veröffentlichung
- 19
- Zeitschrift
- BIOCHEMISTRY
- Paginierung
- 1845 - 1853
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Titel
- Lipid Bilayer Interactions of Peptidic Supramolecular Polymers and Their Impact on Membrane Permeability and Stability
- Sub types
- Article
- Ausgabe der Zeitschrift
- 59
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Stefanie Pannwitt
- Jonas Kaltbeitzel
- Patrick Ahlers
- Daniel Spitzer
- Nadja Hellmann
- Frank Depoix
- Pol Besenius
- Dirk Schneider
- DOI
- 10.1021/acs.biochem.0c00114
- eISSN
- 1520-4995
- ISSN
- 0006-2960
- Ausgabe der Veröffentlichung
- 19
- Zeitschrift
- Biochemistry
- Sprache
- en
- Online publication date
- 2020
- Paginierung
- 1845 - 1853
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Herausgeber
- American Chemical Society (ACS)
- Herausgeber URL
- http://dx.doi.org/10.1021/acs.biochem.0c00114
- Datum der Datenerfassung
- 2023
- Titel
- Lipid Bilayer Interactions of Peptidic Supramolecular Polymers and Their Impact on Membrane Permeability and Stability
- Ausgabe der Zeitschrift
- 59
Datenquelle: Crossref
- Abstract
- The synthesis and physicochemical characterization of supramolecular polymers with tunable assembly profiles offer exciting opportunities, involving the development of new biomedical carriers. Because synthetic nanocarriers aim to transport substances across or toward cellular membranes, we evaluated the interactions of amphiphilic peptide-based supramolecular polymers with lipid bilayers. Here, we focused on nanorod-like supramolecular polymers, obtained from two <i>C</i><sub>3</sub>-symmetric dendritic peptide amphiphiles with alternating Phe/His sequences, equipped with a peripheral tetraethylene glycol dendron (C3-PH) or charged ethylenediamine end groups (C3-PH+). Triggered by pH changes, these amphiphiles assemble reversibly. Our results show that the supramolecular polymers have an impact on the lipid order in model membranes. Changes in the lipid order were observed depending on the charge state of the amphiphilic building blocks, as well as the chemical composition and physical properties of the bilayer. Furthermore, we further performed cell viability assays with the C3-PH+ and C3-PH supramolecular polymers. For C3-PH, the cell viability and extent of proliferation were decreased and the membrane permeability was enhanced, indicating a strong interaction of the polymer with cellular membranes. The results have implications for the design of novel pH-switchable supramolecular drug carriers and delivery vehicles that can respond to an altered microenvironment of tumorous or inflamed tissue.
- Addresses
- Department of Chemistry, Biochemistry, Johannes Gutenberg University Mainz, Johann-Joachim Becherweg 30, 55128 Mainz, Germany.
- Autoren
- Stefanie Pannwitt
- Jonas Kaltbeitzel
- Patrick Ahlers
- Daniel Spitzer
- Nadja Hellmann
- Frank Depoix
- Pol Besenius
- Dirk Schneider
- DOI
- 10.1021/acs.biochem.0c00114
- eISSN
- 1520-4995
- Externe Identifier
- PubMed Identifier: 32320213
- Funding acknowledgements
- Ministeriums f?r Wissenschaft, Weiterbildung und Kultur:
- Open access
- false
- ISSN
- 0006-2960
- Ausgabe der Veröffentlichung
- 19
- Zeitschrift
- Biochemistry
- Schlüsselwörter
- Cells, Cultured
- Humans
- Macromolecular Substances
- Polymers
- Lipid Bilayers
- Peptides
- Cell Proliferation
- Cell Membrane Permeability
- Cell Survival
- Molecular Structure
- Hydrogen-Ion Concentration
- Particle Size
- Surface Properties
- Nanotubes
- HEK293 Cells
- Hydrophobic and Hydrophilic Interactions
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2020
- Paginierung
- 1845 - 1853
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Datum der Datenerfassung
- 2020
- Titel
- Lipid Bilayer Interactions of Peptidic Supramolecular Polymers and Their Impact on Membrane Permeability and Stability.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 59
Datenquelle: Europe PubMed Central
- Abstract
- The synthesis and physicochemical characterization of supramolecular polymers with tunable assembly profiles offer exciting opportunities, involving the development of new biomedical carriers. Because synthetic nanocarriers aim to transport substances across or toward cellular membranes, we evaluated the interactions of amphiphilic peptide-based supramolecular polymers with lipid bilayers. Here, we focused on nanorod-like supramolecular polymers, obtained from two C3-symmetric dendritic peptide amphiphiles with alternating Phe/His sequences, equipped with a peripheral tetraethylene glycol dendron (C3-PH) or charged ethylenediamine end groups (C3-PH+). Triggered by pH changes, these amphiphiles assemble reversibly. Our results show that the supramolecular polymers have an impact on the lipid order in model membranes. Changes in the lipid order were observed depending on the charge state of the amphiphilic building blocks, as well as the chemical composition and physical properties of the bilayer. Furthermore, we further performed cell viability assays with the C3-PH+ and C3-PH supramolecular polymers. For C3-PH, the cell viability and extent of proliferation were decreased and the membrane permeability was enhanced, indicating a strong interaction of the polymer with cellular membranes. The results have implications for the design of novel pH-switchable supramolecular drug carriers and delivery vehicles that can respond to an altered microenvironment of tumorous or inflamed tissue.
- Autoren
- Stefanie Pannwitt
- Jonas Kaltbeitzel
- Patrick Ahlers
- Daniel Spitzer
- Nadja Hellmann
- Frank Depoix
- Pol Besenius
- Dirk Schneider
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/32320213
- DOI
- 10.1021/acs.biochem.0c00114
- eISSN
- 1520-4995
- Ausgabe der Veröffentlichung
- 19
- Zeitschrift
- Biochemistry
- Schlüsselwörter
- Cell Membrane Permeability
- Cell Proliferation
- Cell Survival
- Cells, Cultured
- HEK293 Cells
- Humans
- Hydrogen-Ion Concentration
- Hydrophobic and Hydrophilic Interactions
- Lipid Bilayers
- Macromolecular Substances
- Molecular Structure
- Nanotubes
- Particle Size
- Peptides
- Polymers
- Surface Properties
- Sprache
- eng
- Country
- United States
- Paginierung
- 1845 - 1853
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Titel
- Lipid Bilayer Interactions of Peptidic Supramolecular Polymers and Their Impact on Membrane Permeability and Stability.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 59
Datenquelle: PubMed
- Beziehungen:
- Eigentum von