Analysis of lung surfactant model systems with time-of-flight secondary ion mass spectrometry

Autoren

Nikolaus Bourdos
Felix Kollmer
Alfred Benninghoven
Michaela Ross
Manfred Sieber
Hans-Joachim Galla

DOI

10.1016/s0006-3495(00)76297-7

ISSN

0006-3495

Ausgabe der Veröffentlichung

1

Zeitschrift

Biophysical Journal

Sprache

en

Paginierung

357 - 369

Datum der Veröffentlichung

2000

Status

Published

Herausgeber

Elsevier BV

Herausgeber URL

http://dx.doi.org/10.1016/s0006-3495(00)76297-7

Datum der Datenerfassung

2021

Titel

Analysis of Lung Surfactant Model Systems with Time-of-Flight Secondary Ion Mass Spectrometry

Ausgabe der Zeitschrift

79

Datenquelle: Crossref

Abstract

An often-used model lung surfactant containing dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylglycerol (DPPG), and the surfactant protein C (SP-C) was analyzed as Langmuir-Blodgett film by spatially resolved time-of-flight secondary ion mass spectrometry (TOF-SIMS) to directly visualize the formation and composition of domains. Binary lipid and lipid/SP-C systems were probed for comparison. TOF-SIMS spectra revealed positive secondary ions (SI) characteristic for DPPC and SP-C, but not for DPPG. SI mapping results in images with domain structures in DPPC/DPPG and DPPG/SP-C, but not in DPPC/SP-C films. We are able to distinguish between the fluid and condensed areas probably due to a matrix effect. These findings correspond with other imaging techniques, fluorescence light microscopy (FLM), scanning force microscopy (SFM), and silver decoration. The ternary mixture DPPC/DPPG/SP-C transferred from the collapse region exhibited SP-C-rich domains surrounding pure lipid areas. The results obtained are in full accordance with our earlier SFM picture of layered protrusions that serve as a compressed reservoir for surfactant material during expansion. Our study demonstrates once more that SP-C plays a unique role in the respiration process.

Addresses

Institut für Biochemie, Westfälische Wilhelms-Universität, D-48149 Münster, Germany.

Autoren

N Bourdos
F Kollmer
A Benninghoven
M Ross
M Sieber
HJ Galla

DOI

10.1016/s0006-3495(00)76297-7

eISSN

1542-0086

Externe Identifier

PubMed Identifier: 10866961
PubMed Central ID: PMC1300939

Open access

false

ISSN

0006-3495

Ausgabe der Veröffentlichung

1

Zeitschrift

Biophysical journal

Schlüsselwörter

Lipid Bilayers
1,2-Dipalmitoylphosphatidylcholine
Phosphatidylglycerols
Proteolipids
Pulmonary Surfactants
Membranes, Artificial
Spectrometry, Mass, Secondary Ion
Surface Properties
Surface Tension
Pressure
Models, Chemical

Sprache

eng

Medium

Print

Paginierung

357 - 369

Datum der Veröffentlichung

2000

Status

Published

Datum der Datenerfassung

2000

Titel

Analysis of lung surfactant model systems with time-of-flight secondary ion mass spectrometry.

Sub types

Research Support, Non-U.S. Gov't
research-article
Journal Article

Ausgabe der Zeitschrift

79

Files

http://www.cell.com/article/S0006349500762977/pdf https://europepmc.org/articles/PMC1300939?pdf=render

Datenquelle: Europe PubMed Central

Abstract

An often-used model lung surfactant containing dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylglycerol (DPPG), and the surfactant protein C (SP-C) was analyzed as Langmuir-Blodgett film by spatially resolved time-of-flight secondary ion mass spectrometry (TOF-SIMS) to directly visualize the formation and composition of domains. Binary lipid and lipid/SP-C systems were probed for comparison. TOF-SIMS spectra revealed positive secondary ions (SI) characteristic for DPPC and SP-C, but not for DPPG. SI mapping results in images with domain structures in DPPC/DPPG and DPPG/SP-C, but not in DPPC/SP-C films. We are able to distinguish between the fluid and condensed areas probably due to a matrix effect. These findings correspond with other imaging techniques, fluorescence light microscopy (FLM), scanning force microscopy (SFM), and silver decoration. The ternary mixture DPPC/DPPG/SP-C transferred from the collapse region exhibited SP-C-rich domains surrounding pure lipid areas. The results obtained are in full accordance with our earlier SFM picture of layered protrusions that serve as a compressed reservoir for surfactant material during expansion. Our study demonstrates once more that SP-C plays a unique role in the respiration process.

Autoren

N Bourdos
F Kollmer
A Benninghoven
M Ross
M Sieber
HJ Galla

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/10866961

DOI

10.1016/S0006-3495(00)76297-7

Externe Identifier

PubMed Central ID: PMC1300939

ISSN

0006-3495

Ausgabe der Veröffentlichung

1

Zeitschrift

Biophys J

Schlüsselwörter

1,2-Dipalmitoylphosphatidylcholine
Lipid Bilayers
Membranes, Artificial
Models, Chemical
Phosphatidylglycerols
Pressure
Proteolipids
Pulmonary Surfactants
Spectrometry, Mass, Secondary Ion
Surface Properties
Surface Tension

Sprache

eng

Country

United States

Paginierung

357 - 369

PII

S0006-3495(00)76297-7

Datum der Veröffentlichung

2000

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2000

Titel

Analysis of lung surfactant model systems with time-of-flight secondary ion mass spectrometry.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

79

Datenquelle: PubMed

Analysis of lung surfactant model systems with time-of-flight secondary ion mass spectrometry

Files

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