Classes of non-conventional tetraspanins defined by alternative splicing
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Tetraspanins emerge as a family of membrane proteins mediating an exceptional broad diversity of functions. The naming refers to their four transmembrane segments, which define the tetraspanins‘ typical membrane topology. In this study, we analyzed alternative splicing of tetraspanins. Besides isoforms with four transmembrane segments, most mRNA sequences are coding for isoforms with one, two or three transmembrane segments, representing structurally mono-, di- and trispanins. Moreover, alternative splicing may alter transmembrane topology, delete parts of the large extracellular loop, or generate alternative N- or C-termini. As a result, we define structure-based classes of non-conventional tetraspanins. The increase in gene products by alternative splicing is associated with an unexpected high structural variability of tetraspanins. We speculate that non-conventional tetraspanins have roles in regulating ER exit and modulating tetraspanin-enriched microdomain function.</jats:p>
- Autoren
- Nikolas Hochheimer
- Ricarda Sies
- Anna C Aschenbrenner
- Dirk Schneider
- Thorsten Lang
- DOI
- 10.1038/s41598-019-50267-0
- eISSN
- 2045-2322
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Scientific Reports
- Sprache
- en
- Artikelnummer
- 14075
- Online publication date
- 2019
- Status
- Published online
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1038/s41598-019-50267-0
- Datum der Datenerfassung
- 2022
- Titel
- Classes of non-conventional tetraspanins defined by alternative splicing
- Ausgabe der Zeitschrift
- 9
Datenquelle: Crossref
- Andere Metadatenquellen:
-
- Abstract
- Tetraspanins emerge as a family of membrane proteins mediating an exceptional broad diversity of functions. The naming refers to their four transmembrane segments, which define the tetraspanins' typical membrane topology. In this study, we analyzed alternative splicing of tetraspanins. Besides isoforms with four transmembrane segments, most mRNA sequences are coding for isoforms with one, two or three transmembrane segments, representing structurally mono-, di- and trispanins. Moreover, alternative splicing may alter transmembrane topology, delete parts of the large extracellular loop, or generate alternative N- or C-termini. As a result, we define structure-based classes of non-conventional tetraspanins. The increase in gene products by alternative splicing is associated with an unexpected high structural variability of tetraspanins. We speculate that non-conventional tetraspanins have roles in regulating ER exit and modulating tetraspanin-enriched microdomain function.
- Addresses
- Department of Membrane Biochemistry, Life & Medical Sciences Institute (LIMES), University of Bonn, Carl-Troll-Straße 31, 53115, Bonn, Germany.
- Autoren
- Nikolas Hochheimer
- Ricarda Sies
- Anna C Aschenbrenner
- Dirk Schneider
- Thorsten Lang
- DOI
- 10.1038/s41598-019-50267-0
- eISSN
- 2045-2322
- Externe Identifier
- PubMed Identifier: 31575878
- PubMed Central ID: PMC6773723
- Open access
- true
- ISSN
- 2045-2322
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Scientific reports
- Schlüsselwörter
- Endoplasmic Reticulum
- Humans
- Membrane Proteins
- Proteomics
- Alternative Splicing
- Structure-Activity Relationship
- Isomerism
- Tetraspanins
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2019
- Open access status
- Open Access
- Paginierung
- 14075
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2019
- Titel
- Classes of non-conventional tetraspanins defined by alternative splicing.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 9
Files
https://www.nature.com/articles/s41598-019-50267-0.pdf https://europepmc.org/articles/PMC6773723?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Tetraspanins emerge as a family of membrane proteins mediating an exceptional broad diversity of functions. The naming refers to their four transmembrane segments, which define the tetraspanins' typical membrane topology. In this study, we analyzed alternative splicing of tetraspanins. Besides isoforms with four transmembrane segments, most mRNA sequences are coding for isoforms with one, two or three transmembrane segments, representing structurally mono-, di- and trispanins. Moreover, alternative splicing may alter transmembrane topology, delete parts of the large extracellular loop, or generate alternative N- or C-termini. As a result, we define structure-based classes of non-conventional tetraspanins. The increase in gene products by alternative splicing is associated with an unexpected high structural variability of tetraspanins. We speculate that non-conventional tetraspanins have roles in regulating ER exit and modulating tetraspanin-enriched microdomain function.
- Date of acceptance
- 2019
- Autoren
- Nikolas Hochheimer
- Ricarda Sies
- Anna C Aschenbrenner
- Dirk Schneider
- Thorsten Lang
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/31575878
- DOI
- 10.1038/s41598-019-50267-0
- eISSN
- 2045-2322
- Externe Identifier
- PubMed Central ID: PMC6773723
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Sci Rep
- Schlüsselwörter
- Alternative Splicing
- Endoplasmic Reticulum
- Humans
- Isomerism
- Membrane Proteins
- Proteomics
- Structure-Activity Relationship
- Tetraspanins
- Sprache
- eng
- Country
- England
- Paginierung
- 14075
- PII
- 10.1038/s41598-019-50267-0
- Datum der Veröffentlichung
- 2019
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Titel
- Classes of non-conventional tetraspanins defined by alternative splicing.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 9
Datenquelle: PubMed
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