Variability of Substrate Specificity of Serum Antibodies Obtained from Patients with Different Autoimmune and Viral Diseases in Reaction of tRNA Hydrolysis.

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Abstract
Recently we have shown that the substrate specificity of catalytic IgG isolated from sera of patients with Hashimoto's thyroiditis, systemic lupus erythematosus (SLE), polyarthritis and hepatitis B for classic poly(N) homopolynucleotide substrates and for specific tRNA(Phe) with compact and stable structure was correlated with the type of disease. At the same time the cleavage specificity was different in comparison with that of all known human RNases. Here we investigated for the first time the hydrolysis by the IgGs isolated from sera of 31 patients with different diseases of the in vitro transcript of human mitochondrial tRNA(Lys) which has less stable structure as compared to tRNA(Phe). The level of activity was strongly dependent on the patient, but in general increased in the order: hepatitis B </= Hashimoto's thyroiditis < SLE. The pH dependencies and various salts effects also varied for Abs from the sera of different patients. Nevertheless, the RNase activity of all IgGs was specifically stimulated by Mg(2+) ions, that essentially completely suppress the activity of all known human RNases. In contrast to the classical substrates, no correlation between patient's IgG cleavage specificity and a specific disease was revealed; each patient demonstrated an individual repertoire of polyclonal RNA-hydrolyzing IgGs independently of the disease.
Addresses
  • Institute of Bioorganic Chemistry, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
Autoren
  • Alexander V Vlassov
  • Mark Helm
  • Catherine Florentz
  • Valery A Naumov
  • Alexander A Breusov
  • Valentina N Buneva
  • Richard Giege
  • Georgy A Nevinsky
Externe Identifier
  • PubMed Identifier: 12687113
Open access
false
ISSN
1028-7221
Ausgabe der Veröffentlichung
1
Zeitschrift
Russian journal of immunology : RJI : official journal of Russian Society of Immunology
Sprache
eng
Medium
Print
Paginierung
25 - 32
Datum der Veröffentlichung
1999
Status
Published
Datum der Datenerfassung
2003
Titel
Variability of Substrate Specificity of Serum Antibodies Obtained from Patients with Different Autoimmune and Viral Diseases in Reaction of tRNA Hydrolysis.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
4

Datenquelle: Europe PubMed Central

Andere Metadatenquellen:
Abstract
Recently we have shown that the substrate specificity of catalytic IgG isolated from sera of patients with Hashimoto's thyroiditis, systemic lupus erythematosus (SLE), polyarthritis and hepatitis B for classic poly(N) homopolynucleotide substrates and for specific tRNA(Phe) with compact and stable structure was correlated with the type of disease. At the same time the cleavage specificity was different in comparison with that of all known human RNases. Here we investigated for the first time the hydrolysis by the IgGs isolated from sera of 31 patients with different diseases of the in vitro transcript of human mitochondrial tRNA(Lys) which has less stable structure as compared to tRNA(Phe). The level of activity was strongly dependent on the patient, but in general increased in the order: hepatitis B </= Hashimoto's thyroiditis < SLE. The pH dependencies and various salts effects also varied for Abs from the sera of different patients. Nevertheless, the RNase activity of all IgGs was specifically stimulated by Mg(2+) ions, that essentially completely suppress the activity of all known human RNases. In contrast to the classical substrates, no correlation between patient's IgG cleavage specificity and a specific disease was revealed; each patient demonstrated an individual repertoire of polyclonal RNA-hydrolyzing IgGs independently of the disease.
Autoren
  • Alexander V Vlassov
  • Mark Helm
  • Catherine Florentz
  • Valery A Naumov
  • Alexander A Breusov
  • Valentina N Buneva
  • Richard Giege
  • Georgy A Nevinsky
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/12687113
ISSN
1028-7221
Ausgabe der Veröffentlichung
1
Zeitschrift
Russ J Immunol
Sprache
eng
Country
Russia (Federation)
Paginierung
25 - 32
PII
00499025
Datum der Veröffentlichung
1999
Status
Published
Titel
Variability of Substrate Specificity of Serum Antibodies Obtained from Patients with Different Autoimmune and Viral Diseases in Reaction of tRNA Hydrolysis.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
4

Datenquelle: PubMed

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