RNA Intramolecular Dynamics by Single‐Molecule FRET
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Investigation of single RNA molecules using fluorescence resonance energy transfer (FRET) is a powerful approach to investigate dynamic and thermodynamic aspects of the folding process of a given RNA. Its application requires interdisciplinary work from the fields of chemistry, biochemistry, and physics. The present work gives detailed instructions on the synthesis of RNA molecules labeled with two fluorescent dyes interacting by FRET, as well as on their investigation by single‐molecule fluorescence spectroscopy. <jats:italic>Curr. Protoc. Nucleic Acid Chem</jats:italic>. 34:11.12.1‐11.12.22. © 2008 by John Wiley & Sons, Inc.</jats:p>
- Autoren
- Martin Hengesbach
- Andrei Kobitski
- Felix Voigts‐Hoffmann
- Carina Frauer
- G Ulrich Nienhaus
- Mark Helm
- DOI
- 10.1002/0471142700.nc1112s34
- eISSN
- 1934-9289
- ISSN
- 1934-9270
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Current Protocols in Nucleic Acid Chemistry
- Sprache
- en
- Online publication date
- 2008
- Datum der Veröffentlichung
- 2008
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1002/0471142700.nc1112s34
- Datum der Datenerfassung
- 2023
- Titel
- RNA Intramolecular Dynamics by Single‐Molecule FRET
- Ausgabe der Zeitschrift
- 34
Datenquelle: Crossref
- Andere Metadatenquellen:
-
- Abstract
- Investigation of single RNA molecules using fluorescence resonance energy transfer (FRET) is a powerful approach to investigate dynamic and thermodynamic aspects of the folding process of a given RNA. Its application requires interdisciplinary work from the fields of chemistry, biochemistry, and physics. The present work gives detailed instructions on the synthesis of RNA molecules labeled with two fluorescent dyes interacting by FRET, as well as on their investigation by single-molecule fluorescence spectroscopy.
- Addresses
- Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Heidelberg, Germany.
- Autoren
- Martin Hengesbach
- Andrei Kobitski
- Felix Voigts-Hoffmann
- Carina Frauer
- G Ulrich Nienhaus
- Mark Helm
- DOI
- 10.1002/0471142700.nc1112s34
- eISSN
- 1934-9289
- Externe Identifier
- PubMed Identifier: 18819081
- Open access
- false
- ISSN
- 1934-9270
- Zeitschrift
- Current protocols in nucleic acid chemistry
- Schlüsselwörter
- RNA
- Fluorescent Dyes
- Spectrometry, Fluorescence
- Fluorescence Resonance Energy Transfer
- Lasers
- Nucleic Acid Conformation
- Thermodynamics
- Sprache
- eng
- Medium
- Paginierung
- Unit - 11.12
- Datum der Veröffentlichung
- 2008
- Status
- Published
- Datum der Datenerfassung
- 2008
- Titel
- RNA intramolecular dynamics by single-molecule FRET.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- Chapter 11
Datenquelle: Europe PubMed Central
- Abstract
- Investigation of single RNA molecules using fluorescence resonance energy transfer (FRET) is a powerful approach to investigate dynamic and thermodynamic aspects of the folding process of a given RNA. Its application requires interdisciplinary work from the fields of chemistry, biochemistry, and physics. The present work gives detailed instructions on the synthesis of RNA molecules labeled with two fluorescent dyes interacting by FRET, as well as on their investigation by single-molecule fluorescence spectroscopy.
- Autoren
- Martin Hengesbach
- Andrei Kobitski
- Felix Voigts-Hoffmann
- Carina Frauer
- G Ulrich Nienhaus
- Mark Helm
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/18819081
- DOI
- 10.1002/0471142700.nc1112s34
- eISSN
- 1934-9289
- Zeitschrift
- Curr Protoc Nucleic Acid Chem
- Schlüsselwörter
- Fluorescence Resonance Energy Transfer
- Fluorescent Dyes
- Lasers
- Nucleic Acid Conformation
- RNA
- Spectrometry, Fluorescence
- Thermodynamics
- Sprache
- eng
- Country
- United States
- Paginierung
- Unit - 11.12
- Datum der Veröffentlichung
- 2008
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2008
- Titel
- RNA intramolecular dynamics by single-molecule FRET.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- Chapter 11
Datenquelle: PubMed
- Beziehungen:
- Eigentum von