Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Abstract
- <jats:p>Naturally occurring cellular RNAs contain an impressive number of chemically distinct modified residues which appear posttranscriptionally, as a result of specific action of the corresponding RNA modification enzymes. Over 100 different chemical modifications have been identified and characterized up to now. Identification of the chemical nature and exact position of these modifications is typically based on 2D-TLC analysis of nucleotide digests, on HPLC coupled with mass spectrometry, or on the use of primer extension by reverse transcriptase. However, many modified nucleotides are silent in reverse transcription, since the presence of additional chemical groups frequently does not change base-pairing properties. In this paper, we give a summary of various chemical approaches exploiting the specific reactivity of modified nucleotides in RNA for their detection.</jats:p>
- Autoren
- Isabelle Behm-Ansmant
- Mark Helm
- Yuri Motorin
- DOI
- 10.4061/2011/408053
- eISSN
- 2090-021X
- Zeitschrift
- Journal of Nucleic Acids
- Sprache
- en
- Paginierung
- 1 - 17
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Herausgeber
- Hindawi Limited
- Herausgeber URL
- http://dx.doi.org/10.4061/2011/408053
- Datum der Datenerfassung
- 2021
- Titel
- Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA
- Ausgabe der Zeitschrift
- 2011
Datenquelle: Crossref
- Andere Metadatenquellen:
-
- Abstract
- Naturally occurring cellular RNAs contain an impressive number of chemically distinct modified residues which appear posttranscriptionally, as a result of specific action of the corresponding RNA modification enzymes. Over 100 different chemical modifications have been identified and characterized up to now. Identification of the chemical nature and exact position of these modifications is typically based on 2D-TLC analysis of nucleotide digests, on HPLC coupled with mass spectrometry, or on the use of primer extension by reverse transcriptase. However, many modified nucleotides are silent in reverse transcription, since the presence of additional chemical groups frequently does not change base-pairing properties. In this paper, we give a summary of various chemical approaches exploiting the specific reactivity of modified nucleotides in RNA for their detection.
- Addresses
- Laboratoire ARN-RNP Maturation-Structure-Fonction, Enzymologie Moléculaire et Structurale (AREMS), UMR 7214 CNRS-UHP, Nancy Université, boulevard des Aiguillettes, BP 70239, 54506 Vandoeuvre-les-Nancy, France.
- Autoren
- Isabelle Behm-Ansmant
- Mark Helm
- Yuri Motorin
- DOI
- 10.4061/2011/408053
- eISSN
- 2090-021X
- Externe Identifier
- PubMed Identifier: 21716696
- PubMed Central ID: PMC3118635
- Open access
- true
- ISSN
- 2090-0201
- Zeitschrift
- Journal of nucleic acids
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2011
- Open access status
- Open Access
- Paginierung
- 408053
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2011
- Titel
- Use of specific chemical reagents for detection of modified nucleotides in RNA.
- Sub types
- review-article
- Journal Article
- Ausgabe der Zeitschrift
- 2011
Files
https://downloads.hindawi.com/journals/jna/2011/408053.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21716696/pdf/?tool=EBI https://europepmc.org/articles/PMC3118635?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Naturally occurring cellular RNAs contain an impressive number of chemically distinct modified residues which appear posttranscriptionally, as a result of specific action of the corresponding RNA modification enzymes. Over 100 different chemical modifications have been identified and characterized up to now. Identification of the chemical nature and exact position of these modifications is typically based on 2D-TLC analysis of nucleotide digests, on HPLC coupled with mass spectrometry, or on the use of primer extension by reverse transcriptase. However, many modified nucleotides are silent in reverse transcription, since the presence of additional chemical groups frequently does not change base-pairing properties. In this paper, we give a summary of various chemical approaches exploiting the specific reactivity of modified nucleotides in RNA for their detection.
- Date of acceptance
- 2011
- Autoren
- Isabelle Behm-Ansmant
- Mark Helm
- Yuri Motorin
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/21716696
- DOI
- 10.4061/2011/408053
- eISSN
- 2090-021X
- Externe Identifier
- PubMed Central ID: PMC3118635
- Zeitschrift
- J Nucleic Acids
- Sprache
- eng
- Country
- United States
- Paginierung
- 408053
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2011
- Titel
- Use of specific chemical reagents for detection of modified nucleotides in RNA.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 2011
Datenquelle: PubMed
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- Eigentum von