In vivo down-regulation of mouse brain capillary P-glycoprotein: A preliminary investigation
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Christina Fuest
- Marion Bankstahl
- Petra Winter
- Mark Helm
- Anton Pekcec
- Heidrun Potschka
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000269968300012&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.neulet.2009.08.014
- eISSN
- 1872-7972
- Externe Identifier
- Clarivate Analytics Document Solution ID: 496JU
- PubMed Identifier: 19679165
- ISSN
- 0304-3940
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- NEUROSCIENCE LETTERS
- Schlüsselwörter
- P-glycoprotein
- Multidrug transporter
- Blood-brain barrier
- Pharmacoresistance
- siRNA
- Epilepsy
- Paginierung
- 47 - 51
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Titel
- In vivo down-regulation of mouse brain capillary P-glycoprotein: A preliminary investigation
- Sub types
- Article
- Ausgabe der Zeitschrift
- 464
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Christina Fuest
- Marion Bankstahl
- Petra Winter
- Mark Helm
- Anton Pekcec
- Heidrun Potschka
- DOI
- 10.1016/j.neulet.2009.08.014
- ISSN
- 0304-3940
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Neuroscience Letters
- Sprache
- en
- Paginierung
- 47 - 51
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.neulet.2009.08.014
- Datum der Datenerfassung
- 2018
- Titel
- In vivo down-regulation of mouse brain capillary P-glycoprotein: A preliminary investigation
- Ausgabe der Zeitschrift
- 464
Datenquelle: Crossref
- Abstract
- Over-expression of blood-brain barrier P-glycoprotein is considered as a major hurdle in the treatment of various CNS disorders. A down-regulation strategy is considered as one means to counteract disease- or therapy-associated induction of P-glycoprotein. Here, we evaluated whether a targeting of P-glycoprotein can be achieved in mouse brain capillary endothelial cells using siRNA. A 4-day treatment paradigm with once daily hydrodynamic intravenous injections of siRNA resulted in a significant reduction of the P-glycoprotein-labeled area in the hippocampal hilus and parietal cortex. P-glycoprotein expression proved to be down-regulated in these brain regions by 31 and 16%, respectively. An impact of siRNA administration on density of brain capillaries was excluded by quantification of the endothelial cell marker GLUT-1. In conclusion, the study provides first preliminary evidence that a down-regulation of P-glycoprotein can be achieved in brain capillary endothelial cells by administration of siRNA in vivo.
- Addresses
- Inst of Pharmacology, Toxicology, and Pharmacy, Ludwig-Maximilians-University Munich, 80539 Munich, Germany.
- Autoren
- Christina Fuest
- Marion Bankstahl
- Petra Winter
- Mark Helm
- Anton Pekcec
- Heidrun Potschka
- DOI
- 10.1016/j.neulet.2009.08.014
- eISSN
- 1872-7972
- Externe Identifier
- PubMed Identifier: 19679165
- Open access
- false
- ISSN
- 0304-3940
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Neuroscience letters
- Schlüsselwörter
- Endothelium, Vascular
- Capillaries
- Brain
- Hippocampus
- Parietal Lobe
- Endothelial Cells
- Animals
- Mice, Inbred BALB C
- Mice
- RNA, Small Interfering
- Immunohistochemistry
- Injections, Intravenous
- Down-Regulation
- Male
- Glucose Transporter Type 1
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2009
- Paginierung
- 47 - 51
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Datum der Datenerfassung
- 2009
- Titel
- In vivo down-regulation of mouse brain capillary P-glycoprotein: a preliminary investigation.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 464
Datenquelle: Europe PubMed Central
- Abstract
- Over-expression of blood-brain barrier P-glycoprotein is considered as a major hurdle in the treatment of various CNS disorders. A down-regulation strategy is considered as one means to counteract disease- or therapy-associated induction of P-glycoprotein. Here, we evaluated whether a targeting of P-glycoprotein can be achieved in mouse brain capillary endothelial cells using siRNA. A 4-day treatment paradigm with once daily hydrodynamic intravenous injections of siRNA resulted in a significant reduction of the P-glycoprotein-labeled area in the hippocampal hilus and parietal cortex. P-glycoprotein expression proved to be down-regulated in these brain regions by 31 and 16%, respectively. An impact of siRNA administration on density of brain capillaries was excluded by quantification of the endothelial cell marker GLUT-1. In conclusion, the study provides first preliminary evidence that a down-regulation of P-glycoprotein can be achieved in brain capillary endothelial cells by administration of siRNA in vivo.
- Date of acceptance
- 2009
- Autoren
- Christina Fuest
- Marion Bankstahl
- Petra Winter
- Mark Helm
- Anton Pekcec
- Heidrun Potschka
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/19679165
- DOI
- 10.1016/j.neulet.2009.08.014
- eISSN
- 1872-7972
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Neurosci Lett
- Schlüsselwörter
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Animals
- Brain
- Capillaries
- Down-Regulation
- Endothelial Cells
- Endothelium, Vascular
- Glucose Transporter Type 1
- Hippocampus
- Immunohistochemistry
- Injections, Intravenous
- Male
- Mice
- Mice, Inbred BALB C
- Parietal Lobe
- RNA, Small Interfering
- Sprache
- eng
- Country
- Ireland
- Paginierung
- 47 - 51
- PII
- S0304-3940(09)01081-7
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2009
- Titel
- In vivo down-regulation of mouse brain capillary P-glycoprotein: a preliminary investigation.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 464
Datenquelle: PubMed
- Beziehungen:
- Eigentum von