5-methylcytosine in RNA: detection, enzymatic formation and biological functions

Autoren

Yuri Motorin
Frank Lyko
Mark Helm

DOI

10.1093/nar/gkp1117

eISSN

1362-4962

ISSN

0305-1048

Ausgabe der Veröffentlichung

5

Zeitschrift

Nucleic Acids Research

Sprache

en

Online publication date

2009

Paginierung

1415 - 1430

Datum der Veröffentlichung

2010

Status

Published

Herausgeber

Oxford University Press (OUP)

Herausgeber URL

http://dx.doi.org/10.1093/nar/gkp1117

Datum der Datenerfassung

2021

Titel

5-methylcytosine in RNA: detection, enzymatic formation and biological functions

Ausgabe der Zeitschrift

38

Datenquelle: Crossref

Abstract

The nucleobase modification 5-methylcytosine (m(5)C) is widespread both in DNA and different cellular RNAs. The functions and enzymatic mechanisms of DNA m(5)C-methylation were extensively studied during the last decades. However, the location, the mechanism of formation and the cellular function(s) of the same modified nucleobase in RNA still remain to be elucidated. The recent development of a bisulfite sequencing approach for efficient m(5)C localization in various RNA molecules puts ribo-m(5)C in a highly privileged position as one of the few RNA modifications whose detection is amenable to PCR-based amplification and sequencing methods. Additional progress in the field also includes the characterization of several specific RNA methyltransferase enzymes in various organisms, and the discovery of a new and unexpected link between DNA and RNA m(5)C-methylation. Numerous putative RNA:m(5)C-MTases have now been identified and are awaiting characterization, including the identification of their RNA substrates and their related cellular functions. In order to bring these recent exciting developments into perspective, this review provides an ordered overview of the detection methods for RNA methylation, of the biochemistry, enzymology and molecular biology of the corresponding modification enzymes, and discusses perspectives for the emerging biological functions of these enzymes.

Addresses

Laboratoire ARN-RNP Maturation-Structure-Fonction, Enzymologie Moléculaire et Structurale (AREMS), UMR 7214 CNRS-UHP Faculté des Sciences et Techniques, Université Henri Poincaré, Nancy 1, Bld des Aiguillettes, BP 70239, 54506 Vandoeuvre-les-Nancy, France.

Autoren

Yuri Motorin
Frank Lyko
Mark Helm

DOI

10.1093/nar/gkp1117

eISSN

1362-4962

Externe Identifier

PubMed Identifier: 20007150
PubMed Central ID: PMC2836557

Open access

true

ISSN

0305-1048

Ausgabe der Veröffentlichung

5

Zeitschrift

Nucleic acids research

Schlüsselwörter

5-Methylcytosine
tRNA Methyltransferases
RNA
Sequence Analysis, RNA
Biocatalysis

Sprache

eng

Medium

Print-Electronic

Online publication date

2009

Open access status

Open Access

Paginierung

1415 - 1430

Datum der Veröffentlichung

2010

Status

Published

Publisher licence

CC BY-NC

Datum der Datenerfassung

2009

Titel

5-methylcytosine in RNA: detection, enzymatic formation and biological functions.

Sub types

Research Support, Non-U.S. Gov't
research-article
Review
Journal Article

Ausgabe der Zeitschrift

38

Files

https://academic.oup.com/nar/article-pdf/38/5/1415/11219925/gkp1117.pdf http://nar.oxfordjournals.org/cgi/reprint/38/5/1415.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20007150/pdf/?tool=EBI https://europepmc.org/articles/PMC2836557?pdf=render

Datenquelle: Europe PubMed Central

Abstract

The nucleobase modification 5-methylcytosine (m(5)C) is widespread both in DNA and different cellular RNAs. The functions and enzymatic mechanisms of DNA m(5)C-methylation were extensively studied during the last decades. However, the location, the mechanism of formation and the cellular function(s) of the same modified nucleobase in RNA still remain to be elucidated. The recent development of a bisulfite sequencing approach for efficient m(5)C localization in various RNA molecules puts ribo-m(5)C in a highly privileged position as one of the few RNA modifications whose detection is amenable to PCR-based amplification and sequencing methods. Additional progress in the field also includes the characterization of several specific RNA methyltransferase enzymes in various organisms, and the discovery of a new and unexpected link between DNA and RNA m(5)C-methylation. Numerous putative RNA:m(5)C-MTases have now been identified and are awaiting characterization, including the identification of their RNA substrates and their related cellular functions. In order to bring these recent exciting developments into perspective, this review provides an ordered overview of the detection methods for RNA methylation, of the biochemistry, enzymology and molecular biology of the corresponding modification enzymes, and discusses perspectives for the emerging biological functions of these enzymes.

Autoren

Yuri Motorin
Frank Lyko
Mark Helm

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/20007150

DOI

10.1093/nar/gkp1117

eISSN

1362-4962

Externe Identifier

PubMed Central ID: PMC2836557

Ausgabe der Veröffentlichung

5

Zeitschrift

Nucleic Acids Res

Schlüsselwörter

5-Methylcytosine
Biocatalysis
RNA
Sequence Analysis, RNA
tRNA Methyltransferases

Sprache

eng

Country

England

Paginierung

1415 - 1430

PII

gkp1117

Datum der Veröffentlichung

2010

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2010

Titel

5-methylcytosine in RNA: detection, enzymatic formation and biological functions.

Sub types

Journal Article
Research Support, Non-U.S. Gov't
Review

Ausgabe der Zeitschrift

38

Datenquelle: PubMed

5-methylcytosine in RNA: detection, enzymatic formation and biological functions

Files

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