Detection of RNA modifications
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Stefanie Kellner
- Juergen Burhenne
- Mark Helm
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000282761300019&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.4161/rna.7.2.11468
- eISSN
- 1555-8584
- Externe Identifier
- Clarivate Analytics Document Solution ID: 661VV
- PubMed Identifier: 20224293
- ISSN
- 1547-6286
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- RNA BIOLOGY
- Schlüsselwörter
- RNA modification
- method
- transcriptome
- analysis
- detection
- quantification
- reactivity
- chromatography
- sequencing
- Paginierung
- 237 - 247
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Titel
- Detection of RNA modifications
- Sub types
- Review
- Ausgabe der Zeitschrift
- 7
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Stefanie Kellner
- Jürgen Burhenne
- Mark Helm
- DOI
- 10.4161/rna.7.2.11468
- eISSN
- 1555-8584
- ISSN
- 1547-6286
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- RNA Biology
- Sprache
- en
- Online publication date
- 2014
- Paginierung
- 237 - 247
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Herausgeber
- Informa UK Limited
- Herausgeber URL
- http://dx.doi.org/10.4161/rna.7.2.11468
- Datum der Datenerfassung
- 2016
- Titel
- Detection of RNA modifications
- Ausgabe der Zeitschrift
- 7
Datenquelle: Crossref
- Abstract
- RNA nucleotide modifications are typically of low abundance and frequently go unnoticed by standard detection methods of molecular biology and cell biology. With a burst of knowledge intruding from such diverse areas as genomics, structural biology, regulation of gene expression and immunology, it becomes increasingly clear that many exciting functions of nucleotide modifications remain to be explored. It follows in turn that the biology of nucleotide modification and editing is a field poised to rapidly gain importance in a variety of fields. The detection and analysis of nucleotide modifications present a clear limitation in this respect. Here, various methods for detection of nucleotide modifications are discussed based on three discriminating principles, namely physicochemical properties, enzymatic turnover and chemical reactivity. Because the full extent of nucleotide modification across the various RNA species remains ill understood, emphasis is placed on high-throughput techniques with a potential to screen entire transcriptomes.
- Addresses
- Institute of Pharmacy and Biochemistry, University of Mainz, Mainz, Germany.
- Autoren
- Stefanie Kellner
- Jürgen Burhenne
- Mark Helm
- DOI
- 10.4161/rna.7.2.11468
- eISSN
- 1555-8584
- Externe Identifier
- PubMed Identifier: 20224293
- Open access
- false
- ISSN
- 1547-6286
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- RNA biology
- Schlüsselwörter
- Enzymes
- Nucleotides
- RNA
- Molecular Biology
- RNA Processing, Post-Transcriptional
- Base Sequence
- Molecular Sequence Data
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2010
- Paginierung
- 237 - 247
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Datum der Datenerfassung
- 2010
- Titel
- Detection of RNA modifications.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 7
Datenquelle: Europe PubMed Central
- Abstract
- RNA nucleotide modifications are typically of low abundance and frequently go unnoticed by standard detection methods of molecular biology and cell biology. With a burst of knowledge intruding from such diverse areas as genomics, structural biology, regulation of gene expression and immunology, it becomes increasingly clear that many exciting functions of nucleotide modifications remain to be explored. It follows in turn that the biology of nucleotide modification and editing is a field poised to rapidly gain importance in a variety of fields. The detection and analysis of nucleotide modifications present a clear limitation in this respect. Here, various methods for detection of nucleotide modifications are discussed based on three discriminating principles, namely physicochemical properties, enzymatic turnover and chemical reactivity. Because the full extent of nucleotide modification across the various RNA species remains ill understood, emphasis is placed on high-throughput techniques with a potential to screen entire transcriptomes.
- Autoren
- Stefanie Kellner
- Jürgen Burhenne
- Mark Helm
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/20224293
- DOI
- 10.4161/rna.7.2.11468
- eISSN
- 1555-8584
- Ausgabe der Veröffentlichung
- 2
- Zeitschrift
- RNA Biol
- Schlüsselwörter
- Base Sequence
- Enzymes
- Molecular Biology
- Molecular Sequence Data
- Nucleotides
- RNA
- RNA Processing, Post-Transcriptional
- Sprache
- eng
- Country
- United States
- Paginierung
- 237 - 247
- PII
- 11468
- Datum der Veröffentlichung
- 2010
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2010
- Titel
- Detection of RNA modifications.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 7
Datenquelle: PubMed
- Beziehungen:
- Eigentum von