Measuring Single Small Molecule Binding via Rupture Forces of a Split Aptamer
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Thi-Huong Nguyen
- Lorenz Jan Steinbock
- Hans-Juergen Butt
- Mark Helm
- Ruediger Berger
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000287909200005&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1021/ja1092002
- eISSN
- 1520-5126
- Externe Identifier
- Clarivate Analytics Document Solution ID: 728YA
- PubMed Identifier: 21287983
- ISSN
- 0002-7863
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
- Paginierung
- 2025 - 2027
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Titel
- Measuring Single Small Molecule Binding via Rupture Forces of a Split Aptamer
- Sub types
- Article
- Ausgabe der Zeitschrift
- 133
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Thi-Huong Nguyen
- Lorenz Jan Steinbock
- Hans-Jürgen Butt
- Mark Helm
- Rüdiger Berger
- DOI
- 10.1021/ja1092002
- eISSN
- 1520-5126
- ISSN
- 0002-7863
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- Journal of the American Chemical Society
- Sprache
- en
- Online publication date
- 2011
- Paginierung
- 2025 - 2027
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Herausgeber
- American Chemical Society (ACS)
- Herausgeber URL
- http://dx.doi.org/10.1021/ja1092002
- Datum der Datenerfassung
- 2023
- Titel
- Measuring Single Small Molecule Binding via Rupture Forces of a Split Aptamer
- Ausgabe der Zeitschrift
- 133
Datenquelle: Crossref
- Abstract
- The rupture force of a split (bipartite) aptamer that forms binding pockets for adenosine monophosphate (AMP) was measured by atomic force spectroscopy. Changes in the rupture force were observed in the presence of AMP, while this effect was absent when mutant aptamers or inosine were used. Thus, changes in the rupture force were a direct consequence of specific binding of AMP to the split aptamer. The split aptamer concept allowed the detection of nonlabeled AMP and enabled us to determine the dissociation constant on a single-molecule level.
- Addresses
- Max Planck Institute for Polymer Research, 55128 Mainz, Germany.
- Autoren
- Thi-Huong Nguyen
- Lorenz Jan Steinbock
- Hans-Jürgen Butt
- Mark Helm
- Rüdiger Berger
- DOI
- 10.1021/ja1092002
- eISSN
- 1520-5126
- Externe Identifier
- PubMed Identifier: 21287983
- Open access
- false
- ISSN
- 0002-7863
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- Journal of the American Chemical Society
- Schlüsselwörter
- Adenosine Monophosphate
- Microscopy, Atomic Force
- Protein Binding
- Aptamers, Nucleotide
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2011
- Paginierung
- 2025 - 2027
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Datum der Datenerfassung
- 2011
- Titel
- Measuring single small molecule binding via rupture forces of a split aptamer.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 133
Datenquelle: Europe PubMed Central
- Abstract
- The rupture force of a split (bipartite) aptamer that forms binding pockets for adenosine monophosphate (AMP) was measured by atomic force spectroscopy. Changes in the rupture force were observed in the presence of AMP, while this effect was absent when mutant aptamers or inosine were used. Thus, changes in the rupture force were a direct consequence of specific binding of AMP to the split aptamer. The split aptamer concept allowed the detection of nonlabeled AMP and enabled us to determine the dissociation constant on a single-molecule level.
- Autoren
- Thi-Huong Nguyen
- Lorenz Jan Steinbock
- Hans-Jürgen Butt
- Mark Helm
- Rüdiger Berger
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/21287983
- DOI
- 10.1021/ja1092002
- eISSN
- 1520-5126
- Ausgabe der Veröffentlichung
- 7
- Zeitschrift
- J Am Chem Soc
- Schlüsselwörter
- Adenosine Monophosphate
- Aptamers, Nucleotide
- Microscopy, Atomic Force
- Protein Binding
- Sprache
- eng
- Country
- United States
- Paginierung
- 2025 - 2027
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2011
- Titel
- Measuring single small molecule binding via rupture forces of a split aptamer.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 133
Datenquelle: PubMed
- Beziehungen:
- Eigentum von