Cationic Nanohydrogel Particles as Potential siRNA Carriers for Cellular Delivery
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Lutz Nuhn
- Markus Hirsch
- Bettina Krieg
- Kaloian Koynov
- Karl Fischer
- Manfred Schmidt
- Mark Helm
- Rudolf Zentel
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000301945900033&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1021/nn204116u
- eISSN
- 1936-086X
- Externe Identifier
- Clarivate Analytics Document Solution ID: 914JM
- PubMed Identifier: 22381078
- ISSN
- 1936-0851
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- ACS NANO
- Schlüsselwörter
- nanohydrogel
- block copolymer
- reactive ester
- RAFT polymerization
- self-assembled aggregates
- siRNA delivery
- Paginierung
- 2198 - 2214
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Titel
- Cationic Nanohydrogel Particles as Potential siRNA Carriers for Cellular Delivery
- Sub types
- Article
- Ausgabe der Zeitschrift
- 6
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Lutz Nuhn
- Markus Hirsch
- Bettina Krieg
- Kaloian Koynov
- Karl Fischer
- Manfred Schmidt
- Mark Helm
- Rudolf Zentel
- DOI
- 10.1021/nn204116u
- eISSN
- 1936-086X
- ISSN
- 1936-0851
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- ACS Nano
- Sprache
- en
- Online publication date
- 2012
- Paginierung
- 2198 - 2214
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Herausgeber
- American Chemical Society (ACS)
- Herausgeber URL
- http://dx.doi.org/10.1021/nn204116u
- Datum der Datenerfassung
- 2023
- Titel
- Cationic Nanohydrogel Particles as Potential siRNA Carriers for Cellular Delivery
- Ausgabe der Zeitschrift
- 6
Datenquelle: Crossref
- Abstract
- Oligonucleotides such as short, double-stranded RNA (siRNA) or plasmid DNA (pDNA) promise high potential in gene therapy. For pharmaceutical application, however, adequate drug carriers are required. Among various concepts progressing in the market or final development, nanosized hydrogel particles may serve as novel transport media especially for siRNA. In this work, a new concept of synthesizing polymeric cationic nanohydrogels was developed, which offers a promising strategy to complex and transport siRNA into cells. For this purpose, amphiphilic reactive ester block copolymers were synthesized by RAFT polymerization of pentafluorophenyl methacrylate as reactive ester monomer together with tri(ethylene glycol)methyl ether methacrylate. In polar aprotic solvents, a self-assembly of these polymers could be observed leading to the formation of nanometer-sized polymer aggregates. The resulting superstructures were used to convert the reactive precursor block copolymers with amine-containing cross-linker molecules into covalently stabilized hydrogel particles. Detailed dynamic light scattering studies showed that the structure of the self-assembled aggregates can permanently be locked-in by this process. This method offers a new possibility to synthesize precise nanohydrogels of different size starting from various block copolymers. Moreover, via reactive ester approach, further functionalities could be attached to the nanoparticle, such as fluorescent dyes, which allowed distinct tracing of the hydrogels during complexation with siRNA or cell uptake experiments. In this respect, cellular uptake of the particles themselves as well as with its payload could be detected successfully. Looking ahead, these novel cationic nanohydrogel particles may serve as a new platform for proper siRNA delivery systems.
- Addresses
- Institute of Organic Chemistry, Johannes Gutenberg-University Mainz, Staudingerweg 5, D-55099 Mainz, Germany.
- Autoren
- Lutz Nuhn
- Markus Hirsch
- Bettina Krieg
- Kaloian Koynov
- Karl Fischer
- Manfred Schmidt
- Mark Helm
- Rudolf Zentel
- DOI
- 10.1021/nn204116u
- eISSN
- 1936-086X
- Externe Identifier
- PubMed Identifier: 22381078
- Open access
- false
- ISSN
- 1936-0851
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- ACS nano
- Schlüsselwörter
- Cell Line
- Animals
- Rats
- Amines
- Esters
- RNA, Small Interfering
- Hydrogels
- Drug Carriers
- Solvents
- Molecular Conformation
- Biological Transport
- Models, Molecular
- Nanostructures
- Hydrophobic and Hydrophilic Interactions
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2012
- Paginierung
- 2198 - 2214
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Datum der Datenerfassung
- 2012
- Titel
- Cationic nanohydrogel particles as potential siRNA carriers for cellular delivery.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 6
Datenquelle: Europe PubMed Central
- Abstract
- Oligonucleotides such as short, double-stranded RNA (siRNA) or plasmid DNA (pDNA) promise high potential in gene therapy. For pharmaceutical application, however, adequate drug carriers are required. Among various concepts progressing in the market or final development, nanosized hydrogel particles may serve as novel transport media especially for siRNA. In this work, a new concept of synthesizing polymeric cationic nanohydrogels was developed, which offers a promising strategy to complex and transport siRNA into cells. For this purpose, amphiphilic reactive ester block copolymers were synthesized by RAFT polymerization of pentafluorophenyl methacrylate as reactive ester monomer together with tri(ethylene glycol)methyl ether methacrylate. In polar aprotic solvents, a self-assembly of these polymers could be observed leading to the formation of nanometer-sized polymer aggregates. The resulting superstructures were used to convert the reactive precursor block copolymers with amine-containing cross-linker molecules into covalently stabilized hydrogel particles. Detailed dynamic light scattering studies showed that the structure of the self-assembled aggregates can permanently be locked-in by this process. This method offers a new possibility to synthesize precise nanohydrogels of different size starting from various block copolymers. Moreover, via reactive ester approach, further functionalities could be attached to the nanoparticle, such as fluorescent dyes, which allowed distinct tracing of the hydrogels during complexation with siRNA or cell uptake experiments. In this respect, cellular uptake of the particles themselves as well as with its payload could be detected successfully. Looking ahead, these novel cationic nanohydrogel particles may serve as a new platform for proper siRNA delivery systems.
- Autoren
- Lutz Nuhn
- Markus Hirsch
- Bettina Krieg
- Kaloian Koynov
- Karl Fischer
- Manfred Schmidt
- Mark Helm
- Rudolf Zentel
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/22381078
- DOI
- 10.1021/nn204116u
- eISSN
- 1936-086X
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- ACS Nano
- Schlüsselwörter
- Amines
- Animals
- Biological Transport
- Cell Line
- Drug Carriers
- Esters
- Hydrogels
- Hydrophobic and Hydrophilic Interactions
- Models, Molecular
- Molecular Conformation
- Nanostructures
- RNA, Small Interfering
- Rats
- Solvents
- Sprache
- eng
- Country
- United States
- Paginierung
- 2198 - 2214
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2012
- Titel
- Cationic nanohydrogel particles as potential siRNA carriers for cellular delivery.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 6
Datenquelle: PubMed
- Beziehungen:
- Eigentum von