The Dnmt2 RNA methyltransferase homolog of Geobacter sulfurreducens specifically methylates tRNA-Glu
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Raghuvaran Shanmugam
- Muktak Aklujkar
- Matthias Schaefer
- Richard Reinhardt
- Olaf Nickel
- Gunter Reuter
- Derek R Lovley
- Ann Ehrenhofer-Murray
- Wolfgang Nellen
- Serge Ankri
- Mark Helm
- Tomasz P Jurkowski
- Albert Jeltsch
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000338768100037&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1093/nar/gku256
- eISSN
- 1362-4962
- Externe Identifier
- Clarivate Analytics Document Solution ID: AK9SW
- PubMed Identifier: 24711368
- ISSN
- 0305-1048
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- NUCLEIC ACIDS RESEARCH
- Paginierung
- 6487 - 6496
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Titel
- The Dnmt2 RNA methyltransferase homolog of <i>Geobacter sulfurreducens</i> specifically methylates tRNA-Glu
- Sub types
- Article
- Ausgabe der Zeitschrift
- 42
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Raghuvaran Shanmugam
- Muktak Aklujkar
- Matthias Schäfer
- Richard Reinhardt
- Olaf Nickel
- Gunter Reuter
- Derek R Lovley
- Ann Ehrenhofer-Murray
- Wolfgang Nellen
- Serge Ankri
- Mark Helm
- Tomasz P Jurkowski
- Albert Jeltsch
- DOI
- 10.1093/nar/gku256
- eISSN
- 1362-4962
- ISSN
- 0305-1048
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- Nucleic Acids Research
- Sprache
- en
- Online publication date
- 2014
- Paginierung
- 6487 - 6496
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Herausgeber
- Oxford University Press (OUP)
- Herausgeber URL
- http://dx.doi.org/10.1093/nar/gku256
- Datum der Datenerfassung
- 2018
- Titel
- The Dnmt2 RNA methyltransferase homolog of Geobacter sulfurreducens specifically methylates tRNA-Glu
- Ausgabe der Zeitschrift
- 42
Datenquelle: Crossref
- Abstract
- Dnmt2 enzymes are conserved in eukaryotes, where they methylate C38 of tRNA-Asp with high activity. Here, the activity of one of the very few prokaryotic Dnmt2 homologs from Geobacter species (GsDnmt2) was investigated. GsDnmt2 was observed to methylate tRNA-Asp from flies and mice. Unexpectedly, it had only a weak activity toward its matching Geobacter tRNA-Asp, but methylated Geobacter tRNA-Glu with good activity. In agreement with this result, we show that tRNA-Glu is methylated in Geobacter while the methylation is absent in tRNA-Asp. The activities of Dnmt2 enzymes from Homo sapiens, Drosophila melanogaster, Schizosaccharomyces pombe and Dictyostelium discoideum for methylation of the Geobacter tRNA-Asp and tRNA-Glu were determined showing that all these Dnmt2s preferentially methylate tRNA-Asp. Hence, the GsDnmt2 enzyme has a swapped transfer ribonucleic acid (tRNA) specificity. By comparing the different tRNAs, a characteristic sequence pattern was identified in the variable loop of all preferred tRNA substrates. An exchange of two nucleotides in the variable loop of murine tRNA-Asp converted it to the corresponding variable loop of tRNA-Glu and led to a strong reduction of GsDnmt2 activity. Interestingly, the same loss of activity was observed with human DNMT2, indicating that the variable loop functions as a specificity determinant in tRNA recognition of Dnmt2 enzymes.
- Addresses
- Institute of Biochemistry, Stuttgart University, 70569 Stuttgart, Germany.
- Autoren
- Raghuvaran Shanmugam
- Muktak Aklujkar
- Matthias Schäfer
- Richard Reinhardt
- Olaf Nickel
- Gunter Reuter
- Derek R Lovley
- Ann Ehrenhofer-Murray
- Wolfgang Nellen
- Serge Ankri
- Mark Helm
- Tomasz P Jurkowski
- Albert Jeltsch
- DOI
- 10.1093/nar/gku256
- eISSN
- 1362-4962
- Externe Identifier
- PubMed Identifier: 24711368
- PubMed Central ID: PMC4041430
- Open access
- true
- ISSN
- 0305-1048
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- Nucleic acids research
- Schlüsselwörter
- Animals
- Humans
- Mice
- Geobacter
- tRNA Methyltransferases
- Bacterial Proteins
- RNA, Transfer, Asp
- RNA, Transfer, Glu
- Nucleic Acid Conformation
- Substrate Specificity
- Methylation
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2014
- Open access status
- Open Access
- Paginierung
- 6487 - 6496
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Publisher licence
- CC BY-NC
- Datum der Datenerfassung
- 2014
- Titel
- The Dnmt2 RNA methyltransferase homolog of Geobacter sulfurreducens specifically methylates tRNA-Glu.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 42
Files
https://academic.oup.com/nar/article-pdf/42/10/6487/17423682/gku256.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24711368/pdf/?tool=EBI https://europepmc.org/articles/PMC4041430?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Dnmt2 enzymes are conserved in eukaryotes, where they methylate C38 of tRNA-Asp with high activity. Here, the activity of one of the very few prokaryotic Dnmt2 homologs from Geobacter species (GsDnmt2) was investigated. GsDnmt2 was observed to methylate tRNA-Asp from flies and mice. Unexpectedly, it had only a weak activity toward its matching Geobacter tRNA-Asp, but methylated Geobacter tRNA-Glu with good activity. In agreement with this result, we show that tRNA-Glu is methylated in Geobacter while the methylation is absent in tRNA-Asp. The activities of Dnmt2 enzymes from Homo sapiens, Drosophila melanogaster, Schizosaccharomyces pombe and Dictyostelium discoideum for methylation of the Geobacter tRNA-Asp and tRNA-Glu were determined showing that all these Dnmt2s preferentially methylate tRNA-Asp. Hence, the GsDnmt2 enzyme has a swapped transfer ribonucleic acid (tRNA) specificity. By comparing the different tRNAs, a characteristic sequence pattern was identified in the variable loop of all preferred tRNA substrates. An exchange of two nucleotides in the variable loop of murine tRNA-Asp converted it to the corresponding variable loop of tRNA-Glu and led to a strong reduction of GsDnmt2 activity. Interestingly, the same loss of activity was observed with human DNMT2, indicating that the variable loop functions as a specificity determinant in tRNA recognition of Dnmt2 enzymes.
- Autoren
- Raghuvaran Shanmugam
- Muktak Aklujkar
- Matthias Schäfer
- Richard Reinhardt
- Olaf Nickel
- Gunter Reuter
- Derek R Lovley
- Ann Ehrenhofer-Murray
- Wolfgang Nellen
- Serge Ankri
- Mark Helm
- Tomasz P Jurkowski
- Albert Jeltsch
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/24711368
- DOI
- 10.1093/nar/gku256
- eISSN
- 1362-4962
- Externe Identifier
- PubMed Central ID: PMC4041430
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- Nucleic Acids Res
- Schlüsselwörter
- Animals
- Bacterial Proteins
- Geobacter
- Humans
- Methylation
- Mice
- Nucleic Acid Conformation
- RNA, Transfer, Asp
- RNA, Transfer, Glu
- Substrate Specificity
- tRNA Methyltransferases
- Sprache
- eng
- Country
- England
- Paginierung
- 6487 - 6496
- PII
- gku256
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2014
- Titel
- The Dnmt2 RNA methyltransferase homolog of Geobacter sulfurreducens specifically methylates tRNA-Glu.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 42
Datenquelle: PubMed
- Beziehungen:
- Eigentum von