The Guanidinium Group as a Key Part of Water-Soluble Polymer Carriers for siRNA Complexation and Protection against Degradation
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Ilja Tabujew
- Christoph Freidel
- Bettina Krieg
- Mark Helm
- Kaloian Koynov
- Klaus Muellen
- Kalina Peneva
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000339094900003&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1002/marc.201400120
- eISSN
- 1521-3927
- Externe Identifier
- Clarivate Analytics Document Solution ID: AL4HY
- PubMed Identifier: 24700561
- ISSN
- 1022-1336
- Ausgabe der Veröffentlichung
- 13
- Zeitschrift
- MACROMOLECULAR RAPID COMMUNICATIONS
- Schlüsselwörter
- block copolymers
- GPMA
- HPMA
- siRNA delivery
- microscale thermophoresis
- Paginierung
- 1191 - 1197
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Titel
- The Guanidinium Group as a Key Part of Water-Soluble Polymer Carriers for siRNA Complexation and Protection against Degradation
- Sub types
- Article
- Ausgabe der Zeitschrift
- 35
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:p>Here, the preparation of a novel block copolymer consisting of a statistical copolymer N‐(2‐hydroxypropyl) methacrylamide‐s‐N‐(3‐aminopropyl) methacrylamide and a short terminal 3‐guanidinopropyl methacrylamide block is reported. This polymer structure forms neutral but water‐soluble nanosized complexes with siRNA. The siRNA block copolymer complexes are first analyzed using agarose gel electrophoresis and their size is determined with fluorescence correlation spectroscopy. The protective properties of the polymer against RNA degradation are investigated by treating the siRNA block copolymer complexes with RNase V1. Heparin competition assays confirm the efficient release of the cargo in vitro. In addition, the utilization of microscale thermophoresis is demonstrated for the determination of the binding strength between a fluorescently labeled polyanion and a polymer molecule. <jats:boxed-text content-type="graphic" position="anchor"><jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" mimetype="image/jpg" position="anchor" specific-use="enlarged-web-image" xlink:href="graphic/marc201400120-abs-0001-m.jpg"><jats:alt-text>image</jats:alt-text></jats:graphic></jats:boxed-text></jats:p>
- Autoren
- Ilja Tabujew
- Christoph Freidel
- Bettina Krieg
- Mark Helm
- Kaloian Koynov
- Klaus Müllen
- Kalina Peneva
- DOI
- 10.1002/marc.201400120
- eISSN
- 1521-3927
- ISSN
- 1022-1336
- Ausgabe der Veröffentlichung
- 13
- Zeitschrift
- Macromolecular Rapid Communications
- Sprache
- en
- Online publication date
- 2014
- Paginierung
- 1191 - 1197
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1002/marc.201400120
- Datum der Datenerfassung
- 2023
- Titel
- The Guanidinium Group as a Key Part of Water‐Soluble Polymer Carriers for siRNA Complexation and Protection against Degradation
- Ausgabe der Zeitschrift
- 35
Datenquelle: Crossref
- Abstract
- Here, the preparation of a novel block copolymer consisting of a statistical copolymer N-(2-hydroxypropyl) methacrylamide-s-N-(3-aminopropyl) methacrylamide and a short terminal 3-guanidinopropyl methacrylamide block is reported. This polymer structure forms neutral but water-soluble nanosized complexes with siRNA. The siRNA block copolymer complexes are first analyzed using agarose gel electrophoresis and their size is determined with fluorescence correlation spectroscopy. The protective properties of the polymer against RNA degradation are investigated by treating the siRNA block copolymer complexes with RNase V1. Heparin competition assays confirm the efficient release of the cargo in vitro. In addition, the utilization of microscale thermophoresis is demonstrated for the determination of the binding strength between a fluorescently labeled polyanion and a polymer molecule.
- Addresses
- Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany.
- Autoren
- Ilja Tabujew
- Christoph Freidel
- Bettina Krieg
- Mark Helm
- Kaloian Koynov
- Klaus Müllen
- Kalina Peneva
- DOI
- 10.1002/marc.201400120
- eISSN
- 1521-3927
- Externe Identifier
- PubMed Identifier: 24700561
- Funding acknowledgements
- SFB 1066: Q2
- SFB 1066: A7
- Open access
- false
- ISSN
- 1022-1336
- Ausgabe der Veröffentlichung
- 13
- Zeitschrift
- Macromolecular rapid communications
- Schlüsselwörter
- Water
- Acrylamides
- Guanidine
- Polymers
- Endoribonucleases
- RNA, Small Interfering
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2014
- Paginierung
- 1191 - 1197
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Datum der Datenerfassung
- 2014
- Titel
- The guanidinium group as a key part of water-soluble polymer carriers for siRNA complexation and protection against degradation.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 35
Datenquelle: Europe PubMed Central
- Abstract
- Here, the preparation of a novel block copolymer consisting of a statistical copolymer N-(2-hydroxypropyl) methacrylamide-s-N-(3-aminopropyl) methacrylamide and a short terminal 3-guanidinopropyl methacrylamide block is reported. This polymer structure forms neutral but water-soluble nanosized complexes with siRNA. The siRNA block copolymer complexes are first analyzed using agarose gel electrophoresis and their size is determined with fluorescence correlation spectroscopy. The protective properties of the polymer against RNA degradation are investigated by treating the siRNA block copolymer complexes with RNase V1. Heparin competition assays confirm the efficient release of the cargo in vitro. In addition, the utilization of microscale thermophoresis is demonstrated for the determination of the binding strength between a fluorescently labeled polyanion and a polymer molecule.
- Autoren
- Ilja Tabujew
- Christoph Freidel
- Bettina Krieg
- Mark Helm
- Kaloian Koynov
- Klaus Müllen
- Kalina Peneva
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/24700561
- DOI
- 10.1002/marc.201400120
- eISSN
- 1521-3927
- Ausgabe der Veröffentlichung
- 13
- Zeitschrift
- Macromol Rapid Commun
- Schlüsselwörter
- GPMA
- HPMA
- block copolymers
- microscale thermophoresis
- siRNA delivery
- Acrylamides
- Endoribonucleases
- Guanidine
- Polymers
- RNA, Small Interfering
- Water
- Sprache
- eng
- Country
- Germany
- Paginierung
- 1191 - 1197
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2015
- Titel
- The guanidinium group as a key part of water-soluble polymer carriers for siRNA complexation and protection against degradation.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 35
Datenquelle: PubMed
- Beziehungen:
- Eigentum von