Loss of Anticodon Wobble Uridine Modifications Affects tRNALys Function and Protein Levels in Saccharomyces cerevisiae
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Roland Klassen
- Pia Grunewald
- Kathrin L Thuering
- Christian Eichler
- Mark Helm
- Raffael Schaffrath
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000350689400061&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1371/journal.pone.0119261
- Externe Identifier
- Clarivate Analytics Document Solution ID: CC9KQ
- PubMed Identifier: 25747122
- ISSN
- 1932-6203
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- PLOS ONE
- Artikelnummer
- ARTN e0119261
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Titel
- Loss of Anticodon Wobble Uridine Modifications Affects tRNA<SUP>Lys</SUP> Function and Protein Levels in <i>Saccharomyces cerevisiae</i>
- Sub types
- Article
- Ausgabe der Zeitschrift
- 10
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Roland Klassen
- Pia Grunewald
- Kathrin L Thüring
- Christian Eichler
- Mark Helm
- Raffael Schaffrath
- DOI
- 10.1371/journal.pone.0119261
- Editoren
- Valerie de Crécy-Lagard
- eISSN
- 1932-6203
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- PLOS ONE
- Sprache
- en
- Online publication date
- 2015
- Paginierung
- e0119261 - e0119261
- Status
- Published online
- Herausgeber
- Public Library of Science (PLoS)
- Herausgeber URL
- http://dx.doi.org/10.1371/journal.pone.0119261
- Datum der Datenerfassung
- 2022
- Titel
- Loss of Anticodon Wobble Uridine Modifications Affects tRNALys Function and Protein Levels in Saccharomyces cerevisiae
- Ausgabe der Zeitschrift
- 10
Datenquelle: Crossref
- Abstract
- In eukaryotes, wobble uridines in the anticodons of tRNA(Lys)UUU, tRNA(Glu)UUC and tRNA(Gln)UUG are modified to 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm5s2U). While mutations in subunits of the Elongator complex (Elp1-Elp6), which disable mcm5 side chain formation, or removal of components of the thiolation pathway (Ncs2/Ncs6, Urm1, Uba4) are individually tolerated, the combination of both modification defects has been reported to have lethal effects on Saccharomyces cerevisiae. Contrary to such absolute requirement of mcm5s2U for viability, we demonstrate here that in the S. cerevisiae S288C-derived background, both pathways can be simultaneously inactivated, resulting in combined loss of tRNA anticodon modifications (mcm5U and s2U) without a lethal effect. However, an elp3 disruption strain displays synthetic sick interaction and synergistic temperature sensitivity when combined with either uba4 or urm1 mutations, suggesting major translational defects in the absence of mcm5s2U modifications. Consistent with this notion, we find cellular protein levels drastically decreased in an elp3uba4 double mutant and show that this effect as well as growth phenotypes can be partially rescued by excess of tRNA(Lys)UUU. These results may indicate a global translational or protein homeostasis defect in cells simultaneously lacking mcm5 and s2 wobble uridine modification that could account for growth impairment and mainly originates from tRNA(Lys)UUU hypomodification and malfunction.
- Addresses
- Institut für Biologie, Fachgebiet Mikrobiologie, Universität Kassel, Kassel, Germany.
- Autoren
- Roland Klassen
- Pia Grunewald
- Kathrin L Thüring
- Christian Eichler
- Mark Helm
- Raffael Schaffrath
- DOI
- 10.1371/journal.pone.0119261
- eISSN
- 1932-6203
- Externe Identifier
- PubMed Identifier: 25747122
- PubMed Central ID: PMC4352028
- Open access
- true
- ISSN
- 1932-6203
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- PloS one
- Schlüsselwörter
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- RNA, Fungal
- RNA, Transfer, Lys
- Uridine
- RNA Processing, Post-Transcriptional
- Sprache
- eng
- Medium
- Electronic-eCollection
- Online publication date
- 2015
- Open access status
- Open Access
- Paginierung
- e0119261
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2015
- Titel
- Loss of anticodon wobble uridine modifications affects tRNA(Lys) function and protein levels in Saccharomyces cerevisiae.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 10
Files
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0119261&type=printable https://europepmc.org/articles/PMC4352028?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- In eukaryotes, wobble uridines in the anticodons of tRNA(Lys)UUU, tRNA(Glu)UUC and tRNA(Gln)UUG are modified to 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm5s2U). While mutations in subunits of the Elongator complex (Elp1-Elp6), which disable mcm5 side chain formation, or removal of components of the thiolation pathway (Ncs2/Ncs6, Urm1, Uba4) are individually tolerated, the combination of both modification defects has been reported to have lethal effects on Saccharomyces cerevisiae. Contrary to such absolute requirement of mcm5s2U for viability, we demonstrate here that in the S. cerevisiae S288C-derived background, both pathways can be simultaneously inactivated, resulting in combined loss of tRNA anticodon modifications (mcm5U and s2U) without a lethal effect. However, an elp3 disruption strain displays synthetic sick interaction and synergistic temperature sensitivity when combined with either uba4 or urm1 mutations, suggesting major translational defects in the absence of mcm5s2U modifications. Consistent with this notion, we find cellular protein levels drastically decreased in an elp3uba4 double mutant and show that this effect as well as growth phenotypes can be partially rescued by excess of tRNA(Lys)UUU. These results may indicate a global translational or protein homeostasis defect in cells simultaneously lacking mcm5 and s2 wobble uridine modification that could account for growth impairment and mainly originates from tRNA(Lys)UUU hypomodification and malfunction.
- Date of acceptance
- 2015
- Autoren
- Roland Klassen
- Pia Grunewald
- Kathrin L Thüring
- Christian Eichler
- Mark Helm
- Raffael Schaffrath
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/25747122
- DOI
- 10.1371/journal.pone.0119261
- eISSN
- 1932-6203
- Externe Identifier
- PubMed Central ID: PMC4352028
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- PLoS One
- Schlüsselwörter
- RNA Processing, Post-Transcriptional
- RNA, Fungal
- RNA, Transfer, Lys
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Uridine
- Sprache
- eng
- Country
- United States
- Paginierung
- e0119261
- PII
- PONE-D-14-20779
- Datum der Veröffentlichung
- 2015
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2016
- Titel
- Loss of anticodon wobble uridine modifications affects tRNA(Lys) function and protein levels in Saccharomyces cerevisiae.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 10
Datenquelle: PubMed
- Beziehungen:
- Eigentum von