Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Martin Mueller
- Mark Hartmann
- Isabelle Schuster
- Sebastian Bender
- Kathrin L Thuering
- Mark Helm
- Jon R Katze
- Wolfgang Nellen
- Frank Lyko
- Ann E Ehrenhofer-Murray
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000371237600039&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1093/nar/gkv980
- eISSN
- 1362-4962
- Externe Identifier
- Clarivate Analytics Document Solution ID: DF3IN
- PubMed Identifier: 26424849
- ISSN
- 0305-1048
- Ausgabe der Veröffentlichung
- 22
- Zeitschrift
- NUCLEIC ACIDS RESEARCH
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Titel
- Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine
- Sub types
- Article
- Ausgabe der Zeitschrift
- 43
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Martin Müller
- Mark Hartmann
- Isabelle Schuster
- Sebastian Bender
- Kathrin L Thüring
- Mark Helm
- Jon R Katze
- Wolfgang Nellen
- Frank Lyko
- Ann E Ehrenhofer-Murray
- DOI
- 10.1093/nar/gkv980
- eISSN
- 1362-4962
- ISSN
- 0305-1048
- Ausgabe der Veröffentlichung
- 22
- Zeitschrift
- Nucleic Acids Research
- Sprache
- en
- Online publication date
- 2015
- Paginierung
- 10952 - 10962
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Herausgeber
- Oxford University Press (OUP)
- Herausgeber URL
- http://dx.doi.org/10.1093/nar/gkv980
- Datum der Datenerfassung
- 2022
- Titel
- Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine
- Ausgabe der Zeitschrift
- 43
Datenquelle: Crossref
- Abstract
- Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs. We report here that the activities of two Dnmt2 homologs, Pmt1 from Schizosaccharomyces pombe and DnmA from Dictyostelium discoideum, are strongly stimulated by prior queuosine (Q) modification of the substrate tRNA. In vivo tRNA methylation levels were stimulated by growth of cells in queuine-containing medium; in vitro Pmt1 activity was enhanced on Q-containing RNA; and queuine-stimulated in vivo methylation was abrogated by the absence of the enzyme that inserts queuine into tRNA, eukaryotic tRNA-guanine transglycosylase. Global analysis of tRNA methylation in S. pombe showed a striking selectivity of Pmt1 for tRNA(Asp) methylation, which distinguishes Pmt1 from other Dnmt2 homologs. The present analysis also revealed a novel Pmt1- and Q-independent tRNA methylation site in S. pombe, C34 of tRNA(Pro). Notably, queuine is a micronutrient that is scavenged by higher eukaryotes from the diet and gut microflora. This work therefore reveals an unanticipated route by which the environment can modulate tRNA modification in an organism.
- Addresses
- Institut für Biologie, Humboldt-Universität zu Berlin, 10115 Berlin, Germany.
- Autoren
- Martin Müller
- Mark Hartmann
- Isabelle Schuster
- Sebastian Bender
- Kathrin L Thüring
- Mark Helm
- Jon R Katze
- Wolfgang Nellen
- Frank Lyko
- Ann E Ehrenhofer-Murray
- DOI
- 10.1093/nar/gkv980
- eISSN
- 1362-4962
- Externe Identifier
- PubMed Identifier: 26424849
- PubMed Central ID: PMC4678861
- Open access
- true
- ISSN
- 0305-1048
- Ausgabe der Veröffentlichung
- 22
- Zeitschrift
- Nucleic acids research
- Schlüsselwörter
- Dictyostelium
- Schizosaccharomyces
- Guanine
- Pentosyltransferases
- Micronutrients
- Schizosaccharomyces pombe Proteins
- RNA, Transfer
- RNA, Transfer, Asp
- Methylation
- DNA (Cytosine-5-)-Methyltransferases
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2015
- Open access status
- Open Access
- Paginierung
- 10952 - 10962
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Publisher licence
- CC BY-NC
- Datum der Datenerfassung
- 2015
- Titel
- Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 43
Files
https://academic.oup.com/nar/article-pdf/43/22/10952/16662597/gkv980.pdf https://europepmc.org/articles/PMC4678861?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs. We report here that the activities of two Dnmt2 homologs, Pmt1 from Schizosaccharomyces pombe and DnmA from Dictyostelium discoideum, are strongly stimulated by prior queuosine (Q) modification of the substrate tRNA. In vivo tRNA methylation levels were stimulated by growth of cells in queuine-containing medium; in vitro Pmt1 activity was enhanced on Q-containing RNA; and queuine-stimulated in vivo methylation was abrogated by the absence of the enzyme that inserts queuine into tRNA, eukaryotic tRNA-guanine transglycosylase. Global analysis of tRNA methylation in S. pombe showed a striking selectivity of Pmt1 for tRNA(Asp) methylation, which distinguishes Pmt1 from other Dnmt2 homologs. The present analysis also revealed a novel Pmt1- and Q-independent tRNA methylation site in S. pombe, C34 of tRNA(Pro). Notably, queuine is a micronutrient that is scavenged by higher eukaryotes from the diet and gut microflora. This work therefore reveals an unanticipated route by which the environment can modulate tRNA modification in an organism.
- Date of acceptance
- 2015
- Autoren
- Martin Müller
- Mark Hartmann
- Isabelle Schuster
- Sebastian Bender
- Kathrin L Thüring
- Mark Helm
- Jon R Katze
- Wolfgang Nellen
- Frank Lyko
- Ann E Ehrenhofer-Murray
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/26424849
- DOI
- 10.1093/nar/gkv980
- eISSN
- 1362-4962
- Externe Identifier
- PubMed Central ID: PMC4678861
- Ausgabe der Veröffentlichung
- 22
- Zeitschrift
- Nucleic Acids Res
- Schlüsselwörter
- DNA (Cytosine-5-)-Methyltransferases
- Dictyostelium
- Guanine
- Methylation
- Micronutrients
- Pentosyltransferases
- RNA, Transfer
- RNA, Transfer, Asp
- Schizosaccharomyces
- Schizosaccharomyces pombe Proteins
- Sprache
- eng
- Country
- England
- Paginierung
- 10952 - 10962
- PII
- gkv980
- Datum der Veröffentlichung
- 2015
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2016
- Titel
- Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 43
Datenquelle: PubMed
- Beziehungen:
- Eigentum von