Analysis of RNA modifications by liquid chromatography-tandem mass spectrometry
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Kathrin Thuering
- Katharina Schmid
- Patrick Keller
- Mark Helm
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000383733500007&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.ymeth.2016.03.019
- eISSN
- 1095-9130
- Externe Identifier
- Clarivate Analytics Document Solution ID: DW6CH
- PubMed Identifier: 27020891
- ISSN
- 1046-2023
- Zeitschrift
- METHODS
- Schlüsselwörter
- LC-MS/MS
- Dynamic multiple reaction monitoring
- Neutral loss scan
- RNA modification
- 1-Methyladenosine (m(1)A)
- Paginierung
- 48 - 56
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Titel
- Analysis of RNA modifications by liquid chromatography-tandem mass spectrometry
- Sub types
- Article
- Ausgabe der Zeitschrift
- 107
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Kathrin Thüring
- Katharina Schmid
- Patrick Keller
- Mark Helm
- DOI
- 10.1016/j.ymeth.2016.03.019
- ISSN
- 1046-2023
- Zeitschrift
- Methods
- Sprache
- en
- Paginierung
- 48 - 56
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.ymeth.2016.03.019
- Datum der Datenerfassung
- 2019
- Titel
- Analysis of RNA modifications by liquid chromatography–tandem mass spectrometry
- Ausgabe der Zeitschrift
- 107
Datenquelle: Crossref
- Abstract
- The analysis of RNA modifications is of high importance in order to address a wide range of biological questions. Therefore, a highly sensitive and accurate method such as liquid chromatography-tandem mass spectrometry (LC-MS/MS) has to be available. By using different LC-MS/MS procedures, it is not only possible to quantify very low amounts of RNA modifications, but also to detect probably unknown modified nucleosides. For these cases the dynamic multiple reaction monitoring and the neutral loss scan are the most common techniques. Here, we provide the whole workflow for analyzing RNA samples regarding their modification content. This includes an equipment list, the preparation of required solutions/enzymes and the creation of an internal standard or nucleoside stocks for internal or external calibration. Furthermore, we describe the preparation of RNA samples for the subsequent LC-MS/MS analysis and the corresponding analysis process.
- Addresses
- Institute of Pharmacy and Biochemistry, Johannes Gutenberg-University of Mainz, Staudingerweg 5, 55099 Mainz, Germany. Electronic address: thuering@uni-mainz.de.
- Autoren
- Kathrin Thüring
- Katharina Schmid
- Patrick Keller
- Mark Helm
- DOI
- 10.1016/j.ymeth.2016.03.019
- eISSN
- 1095-9130
- Externe Identifier
- PubMed Identifier: 27020891
- Funding acknowledgements
- DFG: MH3397/13-1
- Open access
- false
- ISSN
- 1046-2023
- Zeitschrift
- Methods (San Diego, Calif.)
- Schlüsselwörter
- Humans
- RNA
- Chromatography, Liquid
- RNA Processing, Post-Transcriptional
- Tandem Mass Spectrometry
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2016
- Paginierung
- 48 - 56
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Datum der Datenerfassung
- 2016
- Titel
- Analysis of RNA modifications by liquid chromatography-tandem mass spectrometry.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 107
Datenquelle: Europe PubMed Central
- Abstract
- The analysis of RNA modifications is of high importance in order to address a wide range of biological questions. Therefore, a highly sensitive and accurate method such as liquid chromatography-tandem mass spectrometry (LC-MS/MS) has to be available. By using different LC-MS/MS procedures, it is not only possible to quantify very low amounts of RNA modifications, but also to detect probably unknown modified nucleosides. For these cases the dynamic multiple reaction monitoring and the neutral loss scan are the most common techniques. Here, we provide the whole workflow for analyzing RNA samples regarding their modification content. This includes an equipment list, the preparation of required solutions/enzymes and the creation of an internal standard or nucleoside stocks for internal or external calibration. Furthermore, we describe the preparation of RNA samples for the subsequent LC-MS/MS analysis and the corresponding analysis process.
- Date of acceptance
- 2016
- Autoren
- Kathrin Thüring
- Katharina Schmid
- Patrick Keller
- Mark Helm
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/27020891
- DOI
- 10.1016/j.ymeth.2016.03.019
- eISSN
- 1095-9130
- Zeitschrift
- Methods
- Schlüsselwörter
- 1-Methyladenosine (m(1)A)
- Dynamic multiple reaction monitoring
- LC–MS/MS
- Neutral loss scan
- RNA modification
- Chromatography, Liquid
- Humans
- RNA
- RNA Processing, Post-Transcriptional
- Tandem Mass Spectrometry
- Sprache
- eng
- Country
- United States
- Paginierung
- 48 - 56
- PII
- S1046-2023(16)30053-6
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2018
- Titel
- Analysis of RNA modifications by liquid chromatography-tandem mass spectrometry.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 107
Datenquelle: PubMed
- Beziehungen:
- Eigentum von