Modulation of Mitochondriotropic Properties of Cyanine Dyes by in Organello Copper-Free Click Reaction

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Inka Negwer
  • Markus Hirsch
  • Stefka Kaloyanova
  • Tom Brown
  • Kalina Peneva
  • Hans-Juergen Butt
  • Kaloian Koynov
  • Mark Helm
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000411329800006&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1002/cbic.201700286
eISSN
1439-7633
Externe Identifier
  • Clarivate Analytics Document Solution ID: FH6ZK
  • PubMed Identifier: 28704573
ISSN
1439-4227
Ausgabe der Veröffentlichung
18
Zeitschrift
CHEMBIOCHEM
Schlüsselwörter
  • click chemistry
  • fluorescent probes
  • FRET
  • in organello synthesis
  • mitochondrial targeting
Paginierung
1814 - 1818
Datum der Veröffentlichung
2017
Status
Published
Titel
Modulation of Mitochondriotropic Properties of Cyanine Dyes by in Organello Copper-Free Click Reaction
Sub types
  • Article
Ausgabe der Zeitschrift
18

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Abstract
<jats:title>Abstract</jats:title><jats:p>Cyanine (Cy) dyes show a general propensity to localize in polarized mitochondria. This mitochondriotropism was used to perform a copper‐free click reaction in the mitochondria of living cells. The in organello reaction of dyes Cy3 and Cy5 led to a product that was easily traceable by Förster resonance energy transfer (FRET). As determined by confocal laser scanning microscopy, the Cy3–Cy5 conjugate showed enhanced retention in mitochondria, relative to that of the starting compounds. This enhancement of a favorable property can be achieved by synthesis in organello, but not outside mitochondria.</jats:p>
Autoren
  • Inka Negwer
  • Markus Hirsch
  • Stefka Kaloyanova
  • Tom Brown
  • Kalina Peneva
  • Hans‐Jürgen Butt
  • Kaloian Koynov
  • Mark Helm
DOI
10.1002/cbic.201700286
eISSN
1439-7633
ISSN
1439-4227
Ausgabe der Veröffentlichung
18
Zeitschrift
ChemBioChem
Sprache
en
Online publication date
2017
Paginierung
1814 - 1818
Datum der Veröffentlichung
2017
Status
Published
Herausgeber
Wiley
Herausgeber URL
http://dx.doi.org/10.1002/cbic.201700286
Datum der Datenerfassung
2023
Titel
Modulation of Mitochondriotropic Properties of Cyanine Dyes by in Organello Copper‐Free Click Reaction
Ausgabe der Zeitschrift
18

Datenquelle: Crossref

Abstract
Cyanine (Cy) dyes show a general propensity to localize in polarized mitochondria. This mitochondriotropism was used to perform a copper-free click reaction in the mitochondria of living cells. The in organello reaction of dyes Cy3 and Cy5 led to a product that was easily traceable by Förster resonance energy transfer (FRET). As determined by confocal laser scanning microscopy, the Cy3-Cy5 conjugate showed enhanced retention in mitochondria, relative to that of the starting compounds. This enhancement of a favorable property can be achieved by synthesis in organello, but not outside mitochondria.
Addresses
  • Pharmaceutical Chemistry, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, 55128, Mainz, Germany.
Autoren
  • Inka Negwer
  • Markus Hirsch
  • Stefka Kaloyanova
  • Tom Brown
  • Kalina Peneva
  • Hans-Jürgen Butt
  • Kaloian Koynov
  • Mark Helm
DOI
10.1002/cbic.201700286
eISSN
1439-7633
Externe Identifier
  • PubMed Identifier: 28704573
Funding acknowledgements
  • Biotechnology and Biological Sciences Research Council: BB/J001694/2
  • Deutsche Forschungsgemeinschaft: SFB 1066; A7
  • Deutsche Forschungsgemeinschaft: SFB 1066; Q2
Open access
false
ISSN
1439-4227
Ausgabe der Veröffentlichung
18
Zeitschrift
Chembiochem : a European journal of chemical biology
Schlüsselwörter
  • Cell Line
  • Mitochondria
  • Animals
  • Rats
  • Copper
  • Carbocyanines
  • Fluorescent Dyes
  • Microscopy, Confocal
  • Fluorescence Resonance Energy Transfer
  • Click Chemistry
Sprache
eng
Medium
Print-Electronic
Online publication date
2017
Paginierung
1814 - 1818
Datum der Veröffentlichung
2017
Status
Published
Datum der Datenerfassung
2017
Titel
Modulation of Mitochondriotropic Properties of Cyanine Dyes by in Organello Copper-Free Click Reaction.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
18

Datenquelle: Europe PubMed Central

Abstract
Cyanine (Cy) dyes show a general propensity to localize in polarized mitochondria. This mitochondriotropism was used to perform a copper-free click reaction in the mitochondria of living cells. The in organello reaction of dyes Cy3 and Cy5 led to a product that was easily traceable by Förster resonance energy transfer (FRET). As determined by confocal laser scanning microscopy, the Cy3-Cy5 conjugate showed enhanced retention in mitochondria, relative to that of the starting compounds. This enhancement of a favorable property can be achieved by synthesis in organello, but not outside mitochondria.
Autoren
  • Inka Negwer
  • Markus Hirsch
  • Stefka Kaloyanova
  • Tom Brown
  • Kalina Peneva
  • Hans-Jürgen Butt
  • Kaloian Koynov
  • Mark Helm
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/28704573
DOI
10.1002/cbic.201700286
eISSN
1439-7633
Ausgabe der Veröffentlichung
18
Zeitschrift
Chembiochem
Schlüsselwörter
  • FRET
  • click chemistry
  • fluorescent probes
  • in organello synthesis
  • mitochondrial targeting
  • Animals
  • Carbocyanines
  • Cell Line
  • Click Chemistry
  • Copper
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes
  • Microscopy, Confocal
  • Mitochondria
  • Rats
Sprache
eng
Country
Germany
Paginierung
1814 - 1818
Datum der Veröffentlichung
2017
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2017
Titel
Modulation of Mitochondriotropic Properties of Cyanine Dyes by in Organello Copper-Free Click Reaction.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
18

Datenquelle: PubMed

Beziehungen:
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