LC-MS Analysis of Methylated RNA

Publikationstyp:
Kapitel
Metadaten:
Autoren
  • Kathrin Thuering
  • Katharina Schmid
  • Patrick Keller
  • Mark Helm
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000417675100002&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1007/978-1-4939-6807-7_1
ISBN-13
978-1-4939-6805-3
Schlüsselwörter
  • RNA methylation
  • LC-MS
  • DMRM
  • NLS
  • SIL-IS
  • External calibration
Paginierung
3 - 18
Buchtitel
RNA METHYLATION: METHODS AND PROTOCOLS
Datum der Veröffentlichung
2017
Status
Published
Titel
LC-MS Analysis of Methylated RNA
Ausgabe der Zeitschrift
1562

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Autoren
  • Kathrin Thüring
  • Katharina Schmid
  • Patrick Keller
  • Mark Helm
DOI
10.1007/978-1-4939-6807-7_1
ISBN-13
9781493968053
Online publication date
2017
Paginierung
3 - 18
Buchtitel
Methods in Molecular Biology
Datum der Veröffentlichung
2017
Status
Published
Herausgeber
Springer New York
Herausgeber URL
http://dx.doi.org/10.1007/978-1-4939-6807-7_1
Datum der Datenerfassung
2017
Titel
LC-MS Analysis of Methylated RNA

Datenquelle: Crossref

Abstract
The detection and quantification of methylated RNA can be beneficial to understand certain cellular regulation processes such as transcriptional modulation of gene expression, immune response, or epigenetic alterations. Therefore, it is necessary to have methods available, which are extremely sensitive and accurate, for instance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Here, we describe the preparation of RNA samples by enzymatic hydrolysis and the subsequent analysis of ribonucleosides by LC-MS/MS via NLS (Neutral loss scan) and DMRM (Dynamic multiple reaction monitoring). Also, we provide variations of these methods including chromatographic techniques and different kinds of quantification.
Addresses
  • Institute of Pharmacy and Biochemistry, Johannes Gutenberg-University of Mainz, Staudingerweg 5, 55099, Mainz, Germany.
Autoren
  • Kathrin Thüring
  • Katharina Schmid
  • Patrick Keller
  • Mark Helm
DOI
10.1007/978-1-4939-6807-7_1
Open access
false
Schlüsselwörter
  • Ribonucleases
  • RNA
  • Calibration
  • Chromatography, Liquid
  • Chromatography, High Pressure Liquid
  • Isotope Labeling
  • Methylation
  • Tandem Mass Spectrometry
  • Statistics as Topic
  • Workflow
Medium
Print
Paginierung
3 - 18
Datum der Veröffentlichung
2017
Status
Published
Datum der Datenerfassung
2017
Titel
LC-MS Analysis of Methylated RNA.
Ausgabe der Zeitschrift
1562

Datenquelle: Europe PubMed Central

Abstract
The detection and quantification of methylated RNA can be beneficial to understand certain cellular regulation processes such as transcriptional modulation of gene expression, immune response, or epigenetic alterations. Therefore, it is necessary to have methods available, which are extremely sensitive and accurate, for instance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Here, we describe the preparation of RNA samples by enzymatic hydrolysis and the subsequent analysis of ribonucleosides by LC-MS/MS via NLS (Neutral loss scan) and DMRM (Dynamic multiple reaction monitoring). Also, we provide variations of these methods including chromatographic techniques and different kinds of quantification.
Autoren
  • Kathrin Thüring
  • Katharina Schmid
  • Patrick Keller
  • Mark Helm
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/28349450
DOI
10.1007/978-1-4939-6807-7_1
Schlüsselwörter
  • DMRM
  • External calibration
  • LC-MS
  • NLS
  • RNA methylation
  • SIL-IS
  • Calibration
  • Chromatography, High Pressure Liquid
  • Chromatography, Liquid
  • Isotope Labeling
  • Methylation
  • RNA
  • Ribonucleases
  • Statistics as Topic
  • Tandem Mass Spectrometry
  • Workflow
Paginierung
3 - 18
Datum der Veröffentlichung
2017
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2018
Titel
LC-MS Analysis of Methylated RNA.
Ausgabe der Zeitschrift
1562

Datenquelle: PubMed

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