Monitoring drug nanocarriers in human blood by near-infrared fluorescence correlation spectroscopy
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Inka Negwer
- Andreas Best
- Meike Schinnerer
- Olga Schaefer
- Leon Capeloa
- Manfred Wagner
- Manfred Schmidt
- Volker Mailaender
- Mark Helm
- Matthias Barz
- Hans-Juergen Butt
- Kaloian Koynov
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000453057400012&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1038/s41467-018-07755-0
- Externe Identifier
- Clarivate Analytics Document Solution ID: HE1TW
- PubMed Identifier: 30546066
- ISSN
- 2041-1723
- Zeitschrift
- NATURE COMMUNICATIONS
- Artikelnummer
- ARTN 5306
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Titel
- Monitoring drug nanocarriers in human blood by near-infrared fluorescence correlation spectroscopy
- Sub types
- Article
- Ausgabe der Zeitschrift
- 9
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Nanocarrier-based drug delivery is a promising therapeutic approach that offers unique possibilities for the treatment of various diseases. However, inside the blood stream, nanocarriers’ properties may change significantly due to interactions with proteins, aggregation, decomposition or premature loss of cargo. Thus, a method for precise, in situ characterization of drug nanocarriers in blood is needed. Here we show how the fluorescence correlation spectroscopy that is a well-established method for measuring the size, loading efficiency and stability of drug nanocarriers in aqueous solutions can be used to directly characterize drug nanocarriers in flowing blood. As the blood is not transparent for visible light and densely crowded with cells, we label the nanocarriers or their cargo with near-infrared fluorescent dyes and fit the experimental autocorrelation functions with an analytical model accounting for the presence of blood cells. The developed methodology contributes towards quantitative understanding of the in vivo behavior of nanocarrier-based therapeutics.</jats:p>
- Autoren
- Inka Negwer
- Andreas Best
- Meike Schinnerer
- Olga Schäfer
- Leon Capeloa
- Manfred Wagner
- Manfred Schmidt
- Volker Mailänder
- Mark Helm
- Matthias Barz
- Hans-Jürgen Butt
- Kaloian Koynov
- DOI
- 10.1038/s41467-018-07755-0
- eISSN
- 2041-1723
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Nature Communications
- Sprache
- en
- Artikelnummer
- 5306
- Online publication date
- 2018
- Status
- Published online
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1038/s41467-018-07755-0
- Datum der Datenerfassung
- 2022
- Titel
- Monitoring drug nanocarriers in human blood by near-infrared fluorescence correlation spectroscopy
- Ausgabe der Zeitschrift
- 9
Datenquelle: Crossref
- Abstract
- Nanocarrier-based drug delivery is a promising therapeutic approach that offers unique possibilities for the treatment of various diseases. However, inside the blood stream, nanocarriers' properties may change significantly due to interactions with proteins, aggregation, decomposition or premature loss of cargo. Thus, a method for precise, in situ characterization of drug nanocarriers in blood is needed. Here we show how the fluorescence correlation spectroscopy that is a well-established method for measuring the size, loading efficiency and stability of drug nanocarriers in aqueous solutions can be used to directly characterize drug nanocarriers in flowing blood. As the blood is not transparent for visible light and densely crowded with cells, we label the nanocarriers or their cargo with near-infrared fluorescent dyes and fit the experimental autocorrelation functions with an analytical model accounting for the presence of blood cells. The developed methodology contributes towards quantitative understanding of the in vivo behavior of nanocarrier-based therapeutics.
- Addresses
- Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany.
- Autoren
- Inka Negwer
- Andreas Best
- Meike Schinnerer
- Olga Schäfer
- Leon Capeloa
- Manfred Wagner
- Manfred Schmidt
- Volker Mailänder
- Mark Helm
- Matthias Barz
- Hans-Jürgen Butt
- Kaloian Koynov
- DOI
- 10.1038/s41467-018-07755-0
- eISSN
- 2041-1723
- Externe Identifier
- PubMed Identifier: 30546066
- PubMed Central ID: PMC6294246
- Open access
- true
- ISSN
- 2041-1723
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Nature communications
- Schlüsselwörter
- Humans
- Drug Carriers
- Fluorescent Dyes
- Spectroscopy, Near-Infrared
- Spectrometry, Fluorescence
- Nanoparticles
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2018
- Open access status
- Open Access
- Paginierung
- 5306
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2018
- Titel
- Monitoring drug nanocarriers in human blood by near-infrared fluorescence correlation spectroscopy.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 9
Files
https://www.nature.com/articles/s41467-018-07755-0.pdf https://europepmc.org/articles/PMC6294246?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Nanocarrier-based drug delivery is a promising therapeutic approach that offers unique possibilities for the treatment of various diseases. However, inside the blood stream, nanocarriers' properties may change significantly due to interactions with proteins, aggregation, decomposition or premature loss of cargo. Thus, a method for precise, in situ characterization of drug nanocarriers in blood is needed. Here we show how the fluorescence correlation spectroscopy that is a well-established method for measuring the size, loading efficiency and stability of drug nanocarriers in aqueous solutions can be used to directly characterize drug nanocarriers in flowing blood. As the blood is not transparent for visible light and densely crowded with cells, we label the nanocarriers or their cargo with near-infrared fluorescent dyes and fit the experimental autocorrelation functions with an analytical model accounting for the presence of blood cells. The developed methodology contributes towards quantitative understanding of the in vivo behavior of nanocarrier-based therapeutics.
- Date of acceptance
- 2018
- Autoren
- Inka Negwer
- Andreas Best
- Meike Schinnerer
- Olga Schäfer
- Leon Capeloa
- Manfred Wagner
- Manfred Schmidt
- Volker Mailänder
- Mark Helm
- Matthias Barz
- Hans-Jürgen Butt
- Kaloian Koynov
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/30546066
- DOI
- 10.1038/s41467-018-07755-0
- eISSN
- 2041-1723
- Externe Identifier
- PubMed Central ID: PMC6294246
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Nat Commun
- Schlüsselwörter
- Drug Carriers
- Fluorescent Dyes
- Humans
- Nanoparticles
- Spectrometry, Fluorescence
- Spectroscopy, Near-Infrared
- Sprache
- eng
- Country
- England
- Paginierung
- 5306
- PII
- 10.1038/s41467-018-07755-0
- Datum der Veröffentlichung
- 2018
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2019
- Titel
- Monitoring drug nanocarriers in human blood by near-infrared fluorescence correlation spectroscopy.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 9
Datenquelle: PubMed
- Beziehungen:
- Eigentum von