A tRNA half modulates translation as stress response in Trypanosoma brucei
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Roger Fricker
- Rebecca Brogli
- Hannes Luidalepp
- Leander Wyss
- Michel Fasnacht
- Oliver Joss
- Marek Zywicki
- Mark Helm
- Andre Schneider
- Marina Cristodero
- Norbert Polacek
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000455354800015&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1038/s41467-018-07949-6
- eISSN
- 2041-1723
- Externe Identifier
- Clarivate Analytics Document Solution ID: HG9UA
- PubMed Identifier: 30631057
- Zeitschrift
- NATURE COMMUNICATIONS
- Artikelnummer
- ARTN 118
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Titel
- A tRNA half modulates translation as stress response in <i>Trypanosoma brucei</i>
- Sub types
- Article
- Ausgabe der Zeitschrift
- 10
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>In the absence of extensive transcription control mechanisms the pathogenic parasite <jats:italic>Trypanosoma brucei</jats:italic> crucially depends on translation regulation to orchestrate gene expression. However, molecular insight into regulating protein biosynthesis is sparse. Here we analyze the small non-coding RNA (ncRNA) interactome of ribosomes in <jats:italic>T. brucei</jats:italic> during different growth conditions and life stages. Ribosome-associated ncRNAs have recently been recognized as unprecedented regulators of ribosome functions. Our data show that the tRNA<jats:sup>Thr</jats:sup> 3´half is produced during nutrient deprivation and becomes one of the most abundant tRNA-derived RNA fragments (tdRs). tRNA<jats:sup>Thr</jats:sup> halves associate with ribosomes and polysomes and stimulate translation by facilitating mRNA loading during stress recovery once starvation conditions ceased. Blocking or depleting the endogenous tRNA<jats:sup>Thr</jats:sup> halves mitigates this stimulatory effect both in vivo and in vitro<jats:italic>. T. brucei</jats:italic> and its close relatives lack the well-described mammalian enzymes for tRNA half processing, thus hinting at a unique tdR biogenesis in these parasites.</jats:p>
- Autoren
- Roger Fricker
- Rebecca Brogli
- Hannes Luidalepp
- Leander Wyss
- Michel Fasnacht
- Oliver Joss
- Marek Zywicki
- Mark Helm
- André Schneider
- Marina Cristodero
- Norbert Polacek
- DOI
- 10.1038/s41467-018-07949-6
- eISSN
- 2041-1723
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Nature Communications
- Sprache
- en
- Artikelnummer
- 118
- Online publication date
- 2019
- Status
- Published online
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1038/s41467-018-07949-6
- Datum der Datenerfassung
- 2022
- Titel
- A tRNA half modulates translation as stress response in Trypanosoma brucei
- Ausgabe der Zeitschrift
- 10
Datenquelle: Crossref
- Abstract
- In the absence of extensive transcription control mechanisms the pathogenic parasite Trypanosoma brucei crucially depends on translation regulation to orchestrate gene expression. However, molecular insight into regulating protein biosynthesis is sparse. Here we analyze the small non-coding RNA (ncRNA) interactome of ribosomes in T. brucei during different growth conditions and life stages. Ribosome-associated ncRNAs have recently been recognized as unprecedented regulators of ribosome functions. Our data show that the tRNA<sup>Thr</sup> 3´half is produced during nutrient deprivation and becomes one of the most abundant tRNA-derived RNA fragments (tdRs). tRNA<sup>Thr</sup> halves associate with ribosomes and polysomes and stimulate translation by facilitating mRNA loading during stress recovery once starvation conditions ceased. Blocking or depleting the endogenous tRNA<sup>Thr</sup> halves mitigates this stimulatory effect both in vivo and in vitro. T. brucei and its close relatives lack the well-described mammalian enzymes for tRNA half processing, thus hinting at a unique tdR biogenesis in these parasites.
- Addresses
- Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012, Bern, Switzerland.
- Autoren
- Roger Fricker
- Rebecca Brogli
- Hannes Luidalepp
- Leander Wyss
- Michel Fasnacht
- Oliver Joss
- Marek Zywicki
- Mark Helm
- André Schneider
- Marina Cristodero
- Norbert Polacek
- DOI
- 10.1038/s41467-018-07949-6
- eISSN
- 2041-1723
- Externe Identifier
- PubMed Identifier: 30631057
- PubMed Central ID: PMC6328589
- Funding acknowledgements
- Swiss National Science Foundation: 166527
- Open access
- true
- ISSN
- 2041-1723
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Nature communications
- Schlüsselwörter
- Ribosomes
- Polyribosomes
- Trypanosoma brucei brucei
- Protozoan Proteins
- RNA, Messenger
- RNA, Protozoan
- RNA, Transfer
- RNA, Transfer, Thr
- Protein Biosynthesis
- Stress, Physiological
- RNA, Small Untranslated
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2019
- Open access status
- Open Access
- Paginierung
- 118
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2019
- Titel
- A tRNA half modulates translation as stress response in Trypanosoma brucei.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 10
Files
https://www.nature.com/articles/s41467-018-07949-6.pdf https://europepmc.org/articles/PMC6328589?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- In the absence of extensive transcription control mechanisms the pathogenic parasite Trypanosoma brucei crucially depends on translation regulation to orchestrate gene expression. However, molecular insight into regulating protein biosynthesis is sparse. Here we analyze the small non-coding RNA (ncRNA) interactome of ribosomes in T. brucei during different growth conditions and life stages. Ribosome-associated ncRNAs have recently been recognized as unprecedented regulators of ribosome functions. Our data show that the tRNAThr 3´half is produced during nutrient deprivation and becomes one of the most abundant tRNA-derived RNA fragments (tdRs). tRNAThr halves associate with ribosomes and polysomes and stimulate translation by facilitating mRNA loading during stress recovery once starvation conditions ceased. Blocking or depleting the endogenous tRNAThr halves mitigates this stimulatory effect both in vivo and in vitro. T. brucei and its close relatives lack the well-described mammalian enzymes for tRNA half processing, thus hinting at a unique tdR biogenesis in these parasites.
- Date of acceptance
- 2018
- Autoren
- Roger Fricker
- Rebecca Brogli
- Hannes Luidalepp
- Leander Wyss
- Michel Fasnacht
- Oliver Joss
- Marek Zywicki
- Mark Helm
- André Schneider
- Marina Cristodero
- Norbert Polacek
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/30631057
- DOI
- 10.1038/s41467-018-07949-6
- eISSN
- 2041-1723
- Externe Identifier
- PubMed Central ID: PMC6328589
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Nat Commun
- Schlüsselwörter
- Polyribosomes
- Protein Biosynthesis
- Protozoan Proteins
- RNA, Messenger
- RNA, Protozoan
- RNA, Small Untranslated
- RNA, Transfer
- RNA, Transfer, Thr
- Ribosomes
- Stress, Physiological
- Trypanosoma brucei brucei
- Sprache
- eng
- Country
- England
- Paginierung
- 118
- PII
- 10.1038/s41467-018-07949-6
- Datum der Veröffentlichung
- 2019
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2019
- Titel
- A tRNA half modulates translation as stress response in Trypanosoma brucei.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 10
Datenquelle: PubMed
- Beziehungen:
- Eigentum von