Absolute Quantification of Noncoding RNA by Microscale Thermophoresis
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Dominik Jacob
- Kathrin Thuering
- Aurellia Galliot
- Virginie Marchand
- Adeline Galvanin
- Akif Ciftci
- Karin Scharmann
- Michael Stock
- Jean-Yves Roignant
- Sebastian A Leidel
- Yuri Motorin
- Raffael Schaffrath
- Roland Klassen
- Mark Helm
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000476610900043&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1002/anie.201814377
- eISSN
- 1521-3773
- Externe Identifier
- Clarivate Analytics Document Solution ID: IK5FK
- PubMed Identifier: 30892798
- ISSN
- 1433-7851
- Ausgabe der Veröffentlichung
- 28
- Zeitschrift
- ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
- Schlüsselwörter
- fluorescence
- hybridization
- microscale thermophoresis
- RNA quantification
- tRNA stability
- Paginierung
- 9565 - 9569
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Titel
- Absolute Quantification of Noncoding RNA by Microscale Thermophoresis
- Sub types
- Article
- Ausgabe der Zeitschrift
- 58
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Accurate quantification of the copy numbers of noncoding RNA has recently emerged as an urgent problem, with impact on fields such as RNA modification research, tissue differentiation, and others. Herein, we present a hybridization‐based approach that uses microscale thermophoresis (MST) as a very fast and highly precise readout to quantify, for example, single tRNA species with a turnaround time of about one hour. We developed MST to quantify the effect of tRNA toxins and of heat stress and RNA modification on single tRNA species. A comparative analysis also revealed significant differences to RNA‐Seq‐based quantification approaches, strongly suggesting a bias due to tRNA modifications in the latter. Further applications include the quantification of rRNA as well as of polyA levels in cellular RNA.</jats:p>
- Autoren
- Dominik Jacob
- Kathrin Thüring
- Aurellia Galliot
- Virginie Marchand
- Adeline Galvanin
- Akif Ciftci
- Karin Scharmann
- Michael Stock
- Jean‐Yves Roignant
- Sebastian A Leidel
- Yuri Motorin
- Raffael Schaffrath
- Roland Klassen
- Mark Helm
- DOI
- 10.1002/anie.201814377
- eISSN
- 1521-3773
- ISSN
- 1433-7851
- Ausgabe der Veröffentlichung
- 28
- Zeitschrift
- Angewandte Chemie International Edition
- Sprache
- en
- Online publication date
- 2019
- Paginierung
- 9565 - 9569
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1002/anie.201814377
- Datum der Datenerfassung
- 2023
- Titel
- Absolute Quantification of Noncoding RNA by Microscale Thermophoresis
- Ausgabe der Zeitschrift
- 58
Datenquelle: Crossref
- Abstract
- Accurate quantification of the copy numbers of noncoding RNA has recently emerged as an urgent problem, with impact on fields such as RNA modification research, tissue differentiation, and others. Herein, we present a hybridization-based approach that uses microscale thermophoresis (MST) as a very fast and highly precise readout to quantify, for example, single tRNA species with a turnaround time of about one hour. We developed MST to quantify the effect of tRNA toxins and of heat stress and RNA modification on single tRNA species. A comparative analysis also revealed significant differences to RNA-Seq-based quantification approaches, strongly suggesting a bias due to tRNA modifications in the latter. Further applications include the quantification of rRNA as well as of polyA levels in cellular RNA.
- Addresses
- Institute of Pharmacy and Biochemistry, Johannes Gutenberg University Mainz, Staudingerweg 5, 55128, Mainz, Germany.
- Autoren
- Dominik Jacob
- Kathrin Thüring
- Aurellia Galliot
- Virginie Marchand
- Adeline Galvanin
- Akif Ciftci
- Karin Scharmann
- Michael Stock
- Jean-Yves Roignant
- Sebastian A Leidel
- Yuri Motorin
- Raffael Schaffrath
- Roland Klassen
- Mark Helm
- DOI
- 10.1002/anie.201814377
- eISSN
- 1521-3773
- Externe Identifier
- PubMed Identifier: 30892798
- PubMed Central ID: PMC6617968
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: SCHA750/20-2
- Deutsche Forschungsgemeinschaft: HE3397/13-2
- Deutsche Forschungsgemeinschaft: HE3397/8-1
- Agence Nationale de la Recherche: HTRNA-Mod ANR-13-ISV8-0001
- Deutsche Forschungsgemeinschaft: KL2937/1-2
- Deutsche Forschungsgemeinschaft: LE3260/2-1
- Deutsche Forschungsgemeinschaft: RO4681/6-1
- Open access
- true
- ISSN
- 1433-7851
- Ausgabe der Veröffentlichung
- 28
- Zeitschrift
- Angewandte Chemie (International ed. in English)
- Schlüsselwörter
- RNA, Untranslated
- Fluorescence
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2019
- Open access status
- Open Access
- Paginierung
- 9565 - 9569
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Publisher licence
- CC BY-NC
- Datum der Datenerfassung
- 2019
- Titel
- Absolute Quantification of Noncoding RNA by Microscale Thermophoresis.
- Sub types
- brief-report
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 58
Files
https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/anie.201814377 https://europepmc.org/articles/PMC6617968?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Accurate quantification of the copy numbers of noncoding RNA has recently emerged as an urgent problem, with impact on fields such as RNA modification research, tissue differentiation, and others. Herein, we present a hybridization-based approach that uses microscale thermophoresis (MST) as a very fast and highly precise readout to quantify, for example, single tRNA species with a turnaround time of about one hour. We developed MST to quantify the effect of tRNA toxins and of heat stress and RNA modification on single tRNA species. A comparative analysis also revealed significant differences to RNA-Seq-based quantification approaches, strongly suggesting a bias due to tRNA modifications in the latter. Further applications include the quantification of rRNA as well as of polyA levels in cellular RNA.
- Autoren
- Dominik Jacob
- Kathrin Thüring
- Aurellia Galliot
- Virginie Marchand
- Adeline Galvanin
- Akif Ciftci
- Karin Scharmann
- Michael Stock
- Jean-Yves Roignant
- Sebastian A Leidel
- Yuri Motorin
- Raffael Schaffrath
- Roland Klassen
- Mark Helm
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/30892798
- DOI
- 10.1002/anie.201814377
- eISSN
- 1521-3773
- Externe Identifier
- PubMed Central ID: PMC6617968
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: HE3397/8-1
- Deutsche Forschungsgemeinschaft: HE3397/13-2
- Deutsche Forschungsgemeinschaft: SCHA750/20-2
- Deutsche Forschungsgemeinschaft: KL2937/1-2
- Deutsche Forschungsgemeinschaft: RO4681/6-1
- Deutsche Forschungsgemeinschaft: LE3260/2-1
- Agence Nationale de la Recherche: HTRNA-Mod ANR-13-ISV8-0001
- Ausgabe der Veröffentlichung
- 28
- Zeitschrift
- Angew Chem Int Ed Engl
- Schlüsselwörter
- RNA quantification
- fluorescence
- hybridization
- microscale thermophoresis
- tRNA stability
- Fluorescence
- RNA, Untranslated
- Sprache
- eng
- Country
- Germany
- Paginierung
- 9565 - 9569
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Titel
- Absolute Quantification of Noncoding RNA by Microscale Thermophoresis.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 58
Datenquelle: PubMed
- Beziehungen:
- Eigentum von