Reversible oxidative dimerization of 4-thiouridines in tRNA isolates

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Larissa Bessler
  • Jonathan Gross
  • Christopher J Kampf
  • Till Opatz
  • Mark Helm
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:001159870600001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1039/d3cb00221g
Externe Identifier
  • Clarivate Analytics Document Solution ID: JR6V8
  • PubMed Identifier: 38456039
ISSN
2633-0679
Ausgabe der Veröffentlichung
3
Zeitschrift
RSC CHEMICAL BIOLOGY
Datum der Veröffentlichung
2024
Status
Published
Titel
Reversible oxidative dimerization of 4-thiouridines in tRNA isolates
Sub types
  • Article
Ausgabe der Zeitschrift
5

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Abstract
<jats:p>In search for new RNA modifications in <jats:italic>E. coli</jats:italic> tRNA we elucidated a disulfide-bridged dimer of 4-thiouridine which was identified as an <jats:italic>ex vivo</jats:italic> artifact and is formed during sample handling in the presence of ambient oxygen.</jats:p>
Autoren
  • Larissa Bessler
  • Jonathan Groß
  • Christopher J Kampf
  • Till Opatz
  • Mark Helm
DOI
10.1039/d3cb00221g
eISSN
2633-0679
Ausgabe der Veröffentlichung
3
Zeitschrift
RSC Chemical Biology
Sprache
en
Online publication date
2024
Paginierung
216 - 224
Status
Published online
Herausgeber
Royal Society of Chemistry (RSC)
Herausgeber URL
http://dx.doi.org/10.1039/d3cb00221g
Datum der Datenerfassung
2024
Titel
Reversible oxidative dimerization of 4-thiouridines in tRNA isolates
Ausgabe der Zeitschrift
5

Datenquelle: Crossref

Abstract
The occurrence of non-canonical nucleoside structures in RNA of biological or synthetic origin has encountered several recent boosts in attention, namely in the context of RNA modifications, and with an eye to RNA vaccines. New nucleoside structures introduce added functionality and function into biopolymers that are otherwise rather homogenous in their chemical structure. Here, we report the discovery of a presumed RNA modification that was identified by combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) with stable isotope labelling as a dimer of the known RNA modification 4-thiouridine (s<sup>4</sup>U). The disulfide-linked structure, which had previously been synthetically introduced into RNA, was here formed spontaneously in isolates of <i>E. coli</i> tRNA. Judicious application of stable isotope labelling suggested that this presumed new RNA modification was rather generated <i>ex vivo</i> by oxidation with ambient oxygen. These findings do not only underscore the need for caution in the discovery of new RNA modifications with respect to artifacts, but also raise awareness of an RNA vulnerability, especially to oxidative damage, during its transport or storage.
Addresses
  • Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University Mainz Staudingerweg 5 55128 Mainz Germany mhelm@uni-mainz.de.
Autoren
  • Larissa Bessler
  • Jonathan Groß
  • Christopher J Kampf
  • Till Opatz
  • Mark Helm
DOI
10.1039/d3cb00221g
eISSN
2633-0679
Externe Identifier
  • PubMed Identifier: 38456039
Funding acknowledgements
  • Deutsche Forschungsgemeinschaft: TP C03 in TRR 319; Project-ID 439669440
  • Deutsche Forschungsgemeinschaft: HE 3397/14-2 in SPP1784
Open access
false
ISSN
2633-0679
Ausgabe der Veröffentlichung
3
Zeitschrift
RSC chemical biology
Sprache
eng
Medium
Electronic-eCollection
Online publication date
2024
Paginierung
216 - 224
Datum der Veröffentlichung
2024
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2024
Titel
Reversible oxidative dimerization of 4-thiouridines in tRNA isolates.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
5

Datenquelle: Europe PubMed Central

Abstract
The occurrence of non-canonical nucleoside structures in RNA of biological or synthetic origin has encountered several recent boosts in attention, namely in the context of RNA modifications, and with an eye to RNA vaccines. New nucleoside structures introduce added functionality and function into biopolymers that are otherwise rather homogenous in their chemical structure. Here, we report the discovery of a presumed RNA modification that was identified by combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) with stable isotope labelling as a dimer of the known RNA modification 4-thiouridine (s4U). The disulfide-linked structure, which had previously been synthetically introduced into RNA, was here formed spontaneously in isolates of E. coli tRNA. Judicious application of stable isotope labelling suggested that this presumed new RNA modification was rather generated ex vivo by oxidation with ambient oxygen. These findings do not only underscore the need for caution in the discovery of new RNA modifications with respect to artifacts, but also raise awareness of an RNA vulnerability, especially to oxidative damage, during its transport or storage.
Date of acceptance
2024
Autoren
  • Larissa Bessler
  • Jonathan Groß
  • Christopher J Kampf
  • Till Opatz
  • Mark Helm
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/38456039
DOI
10.1039/d3cb00221g
eISSN
2633-0679
Externe Identifier
  • PubMed Central ID: PMC10915967
Ausgabe der Veröffentlichung
3
Zeitschrift
RSC Chem Biol
Sprache
eng
Country
England
Paginierung
216 - 224
PII
d3cb00221g
Datum der Veröffentlichung
2024
Status
Published online
Titel
Reversible oxidative dimerization of 4-thiouridines in tRNA isolates.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
5

Datenquelle: PubMed

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