O-Acyl Sugars Protect a Wild Tobacco from Both Native Fungal Pathogens and a Specialist Herbivore

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Thi Luu Van
  • Alexander Weinhold
  • Chhana Ullah
  • Stefanie Dressel
  • Matthias Schoettner
  • Klaus Gase
  • Emmanuel Gaquerel
  • Shuqing Xu
  • Ian T Baldwin
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000402057200027&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1104/pp.16.01904
eISSN
1532-2548
Externe Identifier
  • Clarivate Analytics Document Solution ID: EV8TP
  • PubMed Identifier: 28275149
ISSN
0032-0889
Ausgabe der Veröffentlichung
1
Zeitschrift
PLANT PHYSIOLOGY
Paginierung
370 - 386
Datum der Veröffentlichung
2017
Status
Published
Titel
<i>O</i>-Acyl Sugars Protect a Wild Tobacco from Both Native Fungal Pathogens and a Specialist Herbivore
Sub types
  • Article
Ausgabe der Zeitschrift
174

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Autoren
  • Van Thi Luu
  • Alexander Weinhold
  • Chhana Ullah
  • Stefanie Dressel
  • Matthias Schoettner
  • Klaus Gase
  • Emmanuel Gaquerel
  • Shuqing Xu
  • Ian T Baldwin
DOI
10.1104/pp.16.01904
eISSN
1532-2548
ISSN
0032-0889
Ausgabe der Veröffentlichung
1
Zeitschrift
Plant Physiology
Sprache
en
Online publication date
2017
Paginierung
370 - 386
Datum der Veröffentlichung
2017
Status
Published
Herausgeber
Oxford University Press (OUP)
Herausgeber URL
http://dx.doi.org/10.1104/pp.16.01904
Datum der Datenerfassung
2021
Titel
<i>O-</i>Acyl Sugars Protect a Wild Tobacco from Both Native Fungal Pathogens and a Specialist Herbivore
Ausgabe der Zeitschrift
174

Datenquelle: Crossref

Abstract
<i>O</i>-Acyl sugars (<i>O</i>-AS) are abundant trichome-specific metabolites that function as indirect defenses against herbivores of the wild tobacco <i>Nicotiana attenuata</i>; whether they also function as generalized direct defenses against herbivores and pathogens remains unknown. We characterized natural variation in <i>O-</i>AS among 26 accessions and examined their influence on two native fungal pathogens, <i>Fusarium brachygibbosum</i> U4 and <i>Alternaria</i> sp. U10, and the specialist herbivore <i>Manduca sexta</i> At least 15 different <i>O-</i>AS structures belonging to three classes were found in <i>N. attenuata</i> leaves. A 3-fold quantitative variation in total leaf <i>O-</i>AS was found among the natural accessions. Experiments with natural accessions and crosses between high- and low-<i>O-</i>AS accessions revealed that total <i>O-</i>AS levels were associated with resistance against herbivores and pathogens. Removing <i>O-</i>AS from the leaf surface increased <i>M. sexta</i> growth rate and plant fungal susceptibility. <i>O</i>-AS supplementation in artificial diets and germination medium reduced <i>M. sexta</i> growth and fungal spore germination, respectively. Finally, silencing the expression of a putative branched-chain α-ketoacid dehydrogenase E1 β-subunit-encoding gene (<i>NaBCKDE1B</i>) in the trichomes reduced total leaf <i>O-</i>AS by 20% to 30% and increased susceptibility to <i>Fusarium</i> pathogens. We conclude that <i>O-</i>AS function as direct defenses to protect plants from attack by both native pathogenic fungi and a specialist herbivore and infer that their diversification is likely shaped by the functional interactions among these biotic stresses.
Addresses
  • Department of Molecular Ecology (V.T.L., S.D., M.S., K.G., S.X., I.T.B.) and Department of Biochemistry (C.U.), Max Planck Institute for Chemical Ecology, Jena 07745, Germany.
Autoren
  • Van Thi Luu
  • Alexander Weinhold
  • Chhana Ullah
  • Stefanie Dressel
  • Matthias Schoettner
  • Klaus Gase
  • Emmanuel Gaquerel
  • Shuqing Xu
  • Ian T Baldwin
DOI
10.1104/pp.16.01904
eISSN
1532-2548
Externe Identifier
  • PubMed Identifier: 28275149
Funding acknowledgements
  • European Research Council: 293926
  • Deutsche Forschungsgemeinschaft-funded Collaborative Research Centre: SFB 1127
  • German Research Foundation: FZT 118
  • Max Planck Society:
Open access
false
ISSN
0032-0889
Ausgabe der Veröffentlichung
1
Zeitschrift
Plant physiology
Schlüsselwörter
  • Animals
  • Manduca
  • Alternaria
  • Fusarium
  • Plant Leaves
  • 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
  • Plant Proteins
  • Protein Subunits
  • Plant Diseases
  • Gene Silencing
  • Molecular Structure
  • Acylation
  • Disease Resistance
  • Herbivory
  • Trichomes
  • Sugars
  • Nicotiana
Sprache
eng
Medium
Print-Electronic
Online publication date
2017
Paginierung
370 - 386
Datum der Veröffentlichung
2017
Status
Published
Datum der Datenerfassung
2017
Titel
<i>O-</i>Acyl Sugars Protect a Wild Tobacco from Both Native Fungal Pathogens and a Specialist Herbivore.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
174

Datenquelle: Europe PubMed Central

Abstract
O-Acyl sugars (O-AS) are abundant trichome-specific metabolites that function as indirect defenses against herbivores of the wild tobacco Nicotiana attenuata; whether they also function as generalized direct defenses against herbivores and pathogens remains unknown. We characterized natural variation in O-AS among 26 accessions and examined their influence on two native fungal pathogens, Fusarium brachygibbosum U4 and Alternaria sp. U10, and the specialist herbivore Manduca sexta At least 15 different O-AS structures belonging to three classes were found in N. attenuata leaves. A 3-fold quantitative variation in total leaf O-AS was found among the natural accessions. Experiments with natural accessions and crosses between high- and low-O-AS accessions revealed that total O-AS levels were associated with resistance against herbivores and pathogens. Removing O-AS from the leaf surface increased M. sexta growth rate and plant fungal susceptibility. O-AS supplementation in artificial diets and germination medium reduced M. sexta growth and fungal spore germination, respectively. Finally, silencing the expression of a putative branched-chain α-ketoacid dehydrogenase E1 β-subunit-encoding gene (NaBCKDE1B) in the trichomes reduced total leaf O-AS by 20% to 30% and increased susceptibility to Fusarium pathogens. We conclude that O-AS function as direct defenses to protect plants from attack by both native pathogenic fungi and a specialist herbivore and infer that their diversification is likely shaped by the functional interactions among these biotic stresses.
Date of acceptance
2017
Autoren
  • Van Thi Luu
  • Alexander Weinhold
  • Chhana Ullah
  • Stefanie Dressel
  • Matthias Schoettner
  • Klaus Gase
  • Emmanuel Gaquerel
  • Shuqing Xu
  • Ian T Baldwin
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/28275149
DOI
10.1104/pp.16.01904
eISSN
1532-2548
Externe Identifier
  • PubMed Central ID: PMC5411141
Funding acknowledgements
  • European Research Council: 293926
Ausgabe der Veröffentlichung
1
Zeitschrift
Plant Physiol
Schlüsselwörter
  • 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
  • Acylation
  • Alternaria
  • Animals
  • Disease Resistance
  • Fusarium
  • Gene Silencing
  • Herbivory
  • Manduca
  • Molecular Structure
  • Plant Diseases
  • Plant Leaves
  • Plant Proteins
  • Protein Subunits
  • Sugars
  • Nicotiana
  • Trichomes
Sprache
eng
Country
United States
Paginierung
370 - 386
PII
pp.16.01904
Datum der Veröffentlichung
2017
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2018
Titel
O-Acyl Sugars Protect a Wild Tobacco from Both Native Fungal Pathogens and a Specialist Herbivore.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
174

Datenquelle: PubMed

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